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A selective and sensitive off–on probe for palladium and its application for living cell imaging

Chinese Chemical Letters 2016年第2期27卷 205-210页

作者： Ying Long Jia Zhou Mei-Pan Yang Bing-Qin Yang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry Ministry of Education College of Chemistry and Materials ScienceNorthwest University

A new rhodamine B derivative T1 has been rationally synthesized and displayed selective Pd（Ⅱ）-amplified absorbance and fluorescence emission above 540 nm in methanol-water. Upon the addition of Pd（Ⅱ）, the spirolactam ring was unfolded and a 1：1 metal-ligand complex formed, which can be used for ＇＇naked-eyes＂ detection. In addition, fluorescence imaging experiments of Pd^（2＋） in HepG2 living cells showed its valuable application in biological systems.

关键词： Rhodamine B Palladium Fluorescence probe living cell imaging

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Effect of Probe Lifting Height in Jumping Mode AFM for living cell imaging

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Nanomanufacturing and Metrology 2023年第3期6卷 51-59页

作者： Can Cheng Xingyue Wang Jianjun Dong Zuobin Wang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and TechnologyChangchun130022China Centre for Opto/Bio-Nano Measurement and Manufacturing Zhongshan Institute of Changchun University of Science and TechnologyZhongshan528437China Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing Changchun University of Science and TechnologyChangchun130022China JR3CN&IRAC University of BedfordshireLutonLU13JUUK

Atomic force microscopy(AFM)is one of the effective methods for imaging the morphological and physical properties of living cells in a near-physiological ***,several problems caused by the adhesion of living cells and extension of the cell membranes seriously affect the image quality during living cell imaging,hindering the study of living *** this work,jumping mode AFM imaging was used to image living cells at varied probe lifting heights to meet image quality requirements,and image quality related to the probe lifting height is discussed in *** jumping mode was divided into three parts based on the varying heights of the lifted probe,namely near-contact mode,half-jumping mode,and full-jumping mode,and the causes of their imaging drawbacks were *** an appropriate lifting height,the probe can be completely free from the influence of cell adhesion and self-excited oscillation,thus avoiding the occurrence of“trail”phenomena and invalid points in the imaging of living cells and improving the image ***,this work provides a new approach to calculating the lateral force through the adhesion of trace and retrace scanning at a low height,which is important for studying the extension characteristics of the cell membrane.

关键词： Atomic force microscopy Jumping mode Lifting height living cell imaging

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Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology

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Chemical Research in Chinese Universities 2024年第6期40卷 978-986页

作者： LIU Xiangyu ZHANG Jinrui XU Haijiao SHAO Lina WANG Hongda State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022P.R.China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026P.R.China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237P.R.China

The intracellular logistics system,consisting of vesicles,plays a crucial role in cellular ***,there is a lack of research on the types and functions of intracellular vesicles,and new technologies are needed for further ***,researchers have discovered a new subcellular structure known as Dark-vacuole *** composition,function,and potential synergy with other organelles of these Dark-vacuole bodies remain *** this study,we utilized the high-resolution label-free Fourier ptychographic microscopy,developed by our research group,along with fluorescence confocal technology,to study and analyze Dark-vacuole *** findings provide evidence of the influence of Dark-vacuole bodies on the morphology,distribution,movement,and cell cycle of living ***,we analyzed the effects of drug induced stimulation of lipid drops and endosomes,promotion of cell endocytosis,and induction of cellular senescence on Dark-vacuole *** results indicate that Dark-vacuole bodies show little correlation with lipid drops and endocytosis vesicles,but are significantly associated with late ***,cellular senescence leads to a significant increase in the number and size of Dark-vacuole *** study serves as a foundation for further confirming the nature of Dark-vacuole bodies as new organelles.

关键词： Dark-vacuole body Subcellular Organelle Label-free Fourier ptychographic microscopy(FPM) living cell imaging

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DNA tetrahedron-based split aptamer probes for reliable imaging of ATP in living cells

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Chinese Chemical Letters 2023年第3期34卷 153-156页

作者： Lie Li Jie Wang Huishan Jiang Xiaohong Wen Mei Yang Suping Li Qiuping Guo Kemin Wang State Key Laboratory of Chemo/Biosensing and Chemometrics College of BiologyCollege of Chemistry and Chemical EngineeringHunan UniversityState Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan ProvinceChangsha 410082China

Accurate detection and imaging of adenosine triphosphate(ATP)expression levels in living cells is of great value for understanding cell metabolism,physiological activities,and pathologic ***,we developed a DNA tetrahedron-based split aptamer probe(TD probe)for ratiometric fluorescence imaging of ATP in living *** TD probe is constructed by hybridizing two split ATP aptamer probes(Apt-a and Apt-b)to a DNA tetrahedron assembled by four DNA oligonucleotides(T1,T2,T3 and T4).In the presence of ATP,the TD probe will alter its structure from the open to closed state,thus bringing the separated donor and acceptor fluorophores into close proximity for high fluorescence resonance energy transfer(FRET)*** TD probe exhibits low cytotoxicity,efficient cell internalization and good biological ***,based on the FRET“off”to“on”signal output mode,the TD probe can effectively avoid false-positive signals from complex biological matrices,which is significant for long-term reliable imaging in living *** addition,by changing the split aptamers attached to DNA tetrahedron,the proposed strategy may be extended for detecting various intracellular ***,this strategy provides a valuable sensing platform for biomarkers analysis in living cells,thus having great potential for early clinical diagnosis and therapeutic evaluation.

关键词： Adenosine triphosphate DNA tetrahedron Split aptamer Fluorescence resonance energy transfer living cell imaging

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