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Simultaneous determination of seven bioactive constituents in liuwei wuling tablet by HPLC coupled with DAD

Journal of Chinese Pharmaceutical Sciences 2017年第4期26卷 304-311页

作者：程玲汪小涵申宝德徐平华沈成英郑娟廖魏波徐荣袁海龙空军总医院药学部北京100142 成都中医药大学药学院四川成都611137

We employed high performance liquid chromatography （HPLC） method coupled with diode array detector （DAD） for the determination of seven major bioactive compounds （forsythoside A, specnuezhenide, phillyrin, schisandrin, schisantherin A, deoxyschizandrin and schisandrin B） in liuwei wuling tablet （LWWLT）. The HPLC baseline separation was carried out on an Inertsil ODS-3 column （250 mm×4.6 mm, 5 μm） at 25 ℃ with a gradient elution system composed of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was set at 230 nm. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0,999 within the tested concentration range. The limits of detection and quantification of each compound were in the range of 0.03-0.28 μg/mL and 0.11-0.87μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery of all seven constituents ranged from 96.71% to 103.9%. The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co., Ltd. These results would make foundations for quality control and the further pharmacology study of LWWLT.

关键词： Simultaneous determination Seven bioactive constituents liuwei wuling tablet HPLC-DAD

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A Simultaneous Determination of Seven Principal Compounds in liuwei wuling tablet Using HPLC-DAD

A Simultaneous Determination of Seven Principal Compounds in...

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2015年中国药学大会暨第十五届中国药师周

作者： Ling Cheng Juan-juan Li Gang Shen Ling Qiu Juan Zheng Li-hong Zhang Na Wu Jin Han Hai-long Yuan Pharmacy College Chengdu University of Traditional Chinese Medicine 302 Hospital of PLA&PLA Institute of Chinese Materia Medica

An effective high-performance liquid chromatography method coupled with diode array detection（HPLC-DAD） was established for the simultaneous determination of seven major bioactive compounds in liuwei wuling tablet（LWWLT）, including forsythoside A, specnuezhenide, phillyrin schisandrin, schisantherin A, deoxyschizandrin and schisandrin B. The chromatographic separation was obtained on an Inertsil? ODS-3 column（250 mm×4.6 mm, 5 μm） at 25℃ with a gradient system of 0.1% phosphoric acid and acetonitrile. The wavelength of detector was set at 230 nm. The following parameters including specificity, linearity, limit of detection（LOD）, limit of quantification（LOQ）, precision, stability, repeatability and recovery were tested for evaluating the established analytical method. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0.999 within the test concentration range. The limits of detection and quantification of each compound were in the range of 0.03-0.28 μg/mL and 0.11-0.87 μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery yields of all seven constituents ranged from 96.71-103.9%. The above validated quantificationmethod was successfully applied for the analysis of ten batches of commercial LWWLT. These results would give a support for quality control and the further pharmacology study of LWWLT.

关键词： simultaneous determination seven bioactive constituents liuwei wuling tablet HPLC-DAD

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