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Chinese Journal of Oceanology and Limnology 2017年第6期35卷 1524-1530页

作者：谢世筠李富花张晓军张继泉相建海 Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China

The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus （WSSV） in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of litopenaeus vannamei （LvPT） was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigenTM design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at -27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA （4 pg per shrimp） was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection （hpi）. Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.

关键词： litopenaeus vannamei digestive tract peritrophin-like protein dsRNA interference binding specificity white spot syndrome virus (WSSV) reverse gavage

Molecular cloning and characterization of a threonine/serine protein kinase lvakt from litopenaeus vannamei

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Chinese Journal of Oceanology and Limnology 2014年第4期32卷 792-798页

作者：阮灵伟刘荣雕徐洵施泓 Key Laboratory of Marine Biogenetic Resources Third Institute of OceanographyState Oceanic Administration(SOA )

The phosphatidylinositol 3-kinase(PI3K)-AKT pathway is involved in various cellular functions, including anti-apoptosis, protein synthesis, glucose metabolism and cell cycling. However, the role of the PI3K-AKT pathway in crustaceans remains unclear. In the present study, we cloned and characterized the AKT gene lvakt from litopenaeus vannamei. The 511-residue LVAKT was highly conserved; contained a PH domain, a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L. vannamei. We found, using Real-Time Quantitative PCR(Q-PCR) analysis, that lvakt was upregulated during early white spot syndrome virus(WSSV) infection. Moreover, the PI3K-specific inhibitor, LY294002, reduced viral gene transcription, implying that the PI3K-AKT pathway might be hijacked by WSSV. Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.

关键词： AKT litopenaeus vannamei white spot syndrome virus (WSSV) inhibitor

Transcriptomes of litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids

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Journal of Oceanology and Limnology 2023年第5期41卷 2010-2019页

作者： Wei XIE Guoru DU Honggang DENG Yingchao MA Meirong GAO Hu DUAN Sung YIK YEONG Liying SUI State Key Laboratory of Food Nutrition and Safety Tianjin University of Science&TechnologyTianjin 300457China Asian Regional Artemia Reference Center College of Marine and Environmental SciencesTianjin University of Science and TechnologyTianjin 300457China Institute of Marine Biotechnology(IMB) Universiti Malaysia Terengganu(UMT)Kuala NerusTerengganu 21030Malaysia

Oxidative stress induced by factors such as ammonia nitrogen has become a major issue in shrimp *** effects of carotenoids on the growth and antioxidant capability of litopenaeus vannamei juveniles were investigated in this study using dietary archaeal carotenoids *** four weeks,shrimp were given diets containing 0 mg/kg(Ctrl)and 55.98 mg/kg(Car)archaeal *** archaeal carotenoids significantly enhanced the astaxanthin content in shrimp muscles and carapaces,as well as the superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activity(P<0.05).The malonaldehyde(MDA)content in Car group significantly decreased(P<0.05).The transcriptome analysis was conducted to determine the molecular processes in response to archaeal carotenoids supplementation.A total of 1536 differentially expressed genes(DEGs)were detected,including 538 upregulated DEGs and 998 downregulated *** functional enrichment analysis between Ctrl and Car indicated that 26 GO terms including extracellular region,metabolic process,and proteolysis were *** KEGG pathway enrichment analysis revealed that the amino sugar and nucleotide sugar metabolism,cysteine and methionine metabolism,glycine serine and threonine metabolism,and amino acid biosynthesis were *** carotenoids influenced the expression of several important genes involved in reactive oxygen species(ROS)generation,Nrf2 signaling,and antioxidant *** DEGs were chosen to confirm the RNA-Seq data using *** genes and pathways discovered in this work assist to elucidate the molecular processes through which archaeal carotenoid enhances *** antioxidative system.

关键词： archaeal carotenoid litopenaeus vannamei transcriptome antioxidative mechanism

A Transcriptome Analysis of Neural Tissue of litopenaeus vannamei After Acute Exposure to Alexandrium pacificum

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Journal of Ocean University of China 2024年第2期23卷 529-538页

作者： YANG Huijie HUANG Xianghu MA Yukun HU Zhangxi ZHANG Ning LI Changling ZHANG Yulei Department of Aquaculture College of FisheriesGuangdong Ocean UniversityZhanjiang 524088China Guangdong Laboratory of Marine Ecology Environment Monitoring and Warning Zhanjiang 524088China

Alexandrium pacificum(***)is a typical paralytic shellfish poisonous *** algal blooms(HABs)caused by this species can bring serious environmental problems and economic losses to the aquaculture *** this study,transcriptome sequencing and analyses were performed on the neural tissue of litopenaeus vannamei(***)after acute exposure to *** disrupted solution for 72 h,and differentially expressed genes(DEGs)were *** results showed that,compared with the control samples,300 DEGs were identified in the experimental group,of which 194 were up-regulated,and 106 *** gene ontology(GO)functional enrichment analysis showed that DEGs were significantly enriched in the cortical cytoskeleton organization,troponin complex,amylo-alpha-1,6-glucosidase and thymidine *** encyclopedia of genes and genomes(KEGG)enrichment analysis found that DEGs were mainly enriched in the oxidative phosphorylation process,intercellular tight junctions and *** results showed that the proteoglycans,signaling pathways,and various metabolic processes that regulate cell proliferation,differentiation,and apoptosis all played an essential role in the response of *** to *** toxins.

关键词： litopenaeus vannamei Alexandrium pacificum paralytic shellfish poisoning transcriptome nerve

Identification of a novel C-type lectin from the shrimp litopenaeus vannamei and its role in defense against pathogens infection

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Chinese Journal of Oceanology and Limnology 2011年第5期29卷 942-951页

作者：罗展张继泉李富花张晓军柳承璋相建海 Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences

Acting as one of the pattern recognition receptors （PRRs）, C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene （named LvLecl） was cloned from the shrimp litopenaeus vannamei, The ORF of LvLecl is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain （CRD） at the C-terminal. LvLecl was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLecl transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus （WSSV）. RNAi-based silencing of LvLecl resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic ＂EPN＂（Glu-Pro-Ser） or ＂QPD＂（Gin-Pro-Asp） motif, the recombinant LvLecl, expressed in Escherichia coli BL21 （DE3）, could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLecl might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.

关键词： litopenaeus vannamei innate immune pattern recognition receptor C-type lectin RNAinterference (RNAi) recombinant expression

Glutamate dehydrogenase and Na^+-K^+ ATPase expression and growth response of litopenaeus vannamei to different salinities and dietary protein levels

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Chinese Journal of Oceanology and Limnology 2011年第2期29卷 343-349页

作者：李二超 Leticia ARENA Gabriel LIZAMA Gabriela GAXIOLA Gerard CUZON Carlos ROSAS 陈立侨 Alain VAN WORMHOUDT School of Life Science East China Normal University UMR5178 Station de Biologie Marine du Musum National d'Histoire NaturelleBP225Concarneau 29900France Unidad Multidisciplinaria de Docencia e Investigación Facultad de CienciasUniversidad Nacional Autónoma de MxicoPuerto de abrigo s/nSisal C.P.97350HunucmYucatnMexico IFREMER TahitiFrench Polynesia

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp *** effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(***) were *** involved an examination of growth performance,glutamate dehydrogenase(GDH) and Na+-K+ ATPase mRNA expression,,and GDH activity in muscles and *** experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of *** 20 days,no significant difference was observed in weight gain,though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein ***,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and *** fed with 40% protein at 25 in salinity(optimal conditions) were used as a *** of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the *** fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 *** salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 *** findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein *** dietary protein level could improve the osmoregulation capacity of *** with more energy resources allocated to GDH activity and expression.

关键词： litopenaeus vannamei gene expression glutamate dehydrogenase Na^+-K^+ ATPase protein salinity

Effect of various Na/K ratios in low-salinity well water on growth performance and physiological response of Pacific white shrimp litopenaeus vannamei

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Chinese Journal of Oceanology and Limnology 2014年第5期32卷 991-999页

作者：刘泓宇谭北平杨金芳林颖博迟淑艳董晓慧杨奇慧 Laboratory of Aquatic Animal Nutrition and Feed Fisheries CollegeGuangdong Ocean University Department of Oncology and Pathology Cancer Center KarolinskaR8:04Karolinska Hospital

To investigate the influence of sodium to potassium （Na/IO ratios on the growth performance and physiological response of the Pacific white shrimp （litopenaeus vananmei）, various concentrations of KC1 were added to low-salinity well water （salinity 4） in an 8-week culture trial. Six treatments with NWK ratios of 60：1, 42：1, 33：1, 23：1, 17：1, and 14：1 were replicated in triplicate. The highest weight-gain rate （3 506±48）% and survival rate （89.38±0.88）% was observed in well water with Na/K ratios of 23：1 and 42：1, respectively, while the feed conversion ratio （1.02～0.01）, oxygen consumption, and ammonia-N excretion rate was the lowest in the medium with a Na/K ratio of 23：1. Gill Na＋-K＋-ATPase activity, as an indicator of osmoregulation, peaked in the treatment where the Na/K ratio was 17：1. The total hemocyte count, respiratory burst, and immune-related enzyme activities （ALP, LSZ, PO, and SOD） ofL. vananmei were affected significantly by Na/K ratios （P〈0.05）. After challenged with Vibrio harveyi, the cumulative mortality of shrimp reared in a Na/K ratio of 23：1 （30±14.14）% was significantly lower than the control （75～7.07）%. In conclusion, the addition of K＋ to low-salinity well water in L. vannamei cultures is feasible. Na/K ratios ranging from 23：1 to 33：1 might improve survival and growth. Immunity and disease resistance are also closely related to the Na/K ratio of the low-salinity well water. The findings may contribute to the development of more efficient K^＋ remediation strategies for L. vananmei culture in low-salinity well water.

关键词： litopenaeus vannamei low-salinity well water Na/K ratio osmoregulation nitrogen metabolism immunity

Reducing the Common Environmental Effect on litopenaeus vannamei Body Weight by Rearing Communally at Early Developmental Stages and Using a Reconstructed Pedigree

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Journal of Ocean University of China 2020年第4期19卷 923-930页

作者： KONG Zhangwei KONG Jie HAO Dongchun LU Xia JIAN Tan MENG Xianhong LUO Kun CAO Baoxiang SUI Juan LI Xupeng LUAN Sheng Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China Fisheries College Ocean University of ChinaQingdao 266003China

Heritability estimates may be severely biased when a large common environmental effect on a family arises from a longlasting separate rearing at early stages(SRES)in traditional selective breeding programs,especially when bred populations have weak genetic *** rearing at early stages(CRES)may reduce common environmental effect since all families are reared in the same environment immediately after ***,we compared the effects of CRES and SRES strategies on genetic parameter estimation for harvest body weight in a selective breeding population of litopenaeus vannamei with a small number of half-sib *** parameters of each strategy were estimated by using animal models excluding and including the common environmental effect(Model 1 and Model 2,respectively).Heritability estimates for body weight were 0.21±0.06(P0.05)in Model *** ratio of common environmental variance to phenotypic variance was 0.002±0.000 and 0.071±0.112 for CRES and SRES,*** strategy precisely partitioned the common environmental variance according to likelihood ratio *** heritability for body weight in CRES than in SRES implied that a large common environmental variance was confounded with additive genetic variance and was not effectively partitioned in ***,genetic correlation of body weight between the two strategies was 0.75±0.15,indicating that family rankings truly *** CRES should be followed in the selective breeding program of shrimp,especially in a population with a shallow pedigree and weak genetic ties between families.

关键词： common environmental effect heritability litopenaeus vannamei parentage assignment

BAC end sequencing of Pacific white shrimp litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

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Chinese Journal of Oceanology and Limnology 2012年第3期30卷 456-470页

作者：赵翠张晓军柳承璋郇聘李富花相建海黄超 Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Graduate University of Chinese Academy of Sciences

Little is known about the genome of Pacific white shrimp (litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.

关键词： litopenaeus vannamei BES repetitive sequences SSR genome survey

Heritability of body weight and resistance to ammonia in the Pacific white shrimp litopenaeus vannamei juveniles

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Chinese Journal of Oceanology and Limnology 2016年第5期34卷 1025-1033页

作者：李文嘉卢霞栾生罗坤隋娟孔杰 Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences

Ammonia,toxic to aquaculture organisms,represents a potential problem in aquaculture systems,and the situation is exacerbated in closed and intensive shrimp farming operations,expecially for litopenaeus *** the potential for the genetic improvement of resistance to ammonia in *** requires knowledge of the genetic parameters of this *** heritability of resistance to ammonia was estimated using two descriptors in the present study:the survival time(ST) and the survival status at half lethal time(SS_(50)) for each individual under high ammonia *** heritability of ST and SS_(50) were low(0.154 4±0.044 6 and 0.147 5±0.040 0,respectively),but they were both significantly different from zero(P0.05),suggesting that ST and SS_(50) could be used as suitable indicators for resistance to *** were also positive phenotypic and genetic correlation between resistance to ammonia and body weight,which means that resistance to ammonia can be enhanced by the improvement of husbandry practices that increase the body *** results from the present study suggest that the selection for higher body weight does not have any negative consequences for resistance to *** addition to quantitative genetics,tools from molecular genetics can be applied to selective breeding programs to improve the efficiency of selection for traits with low heritability.

关键词： litopenaeus vannamei heritability resistance to ammonia body weight genetic correlation