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Chemical Research in Chinese Universities 2011年第4期27卷 562-565页

作者： ZHAO Xiao-ping JIN Ye-cheng ZENG Xing ZHANG Bo-li ZHANG Yu-feng Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese Medicine Tianjin 300193 P. R. China College of Preclinical Medicine Zhejiang Chinese Medical University Hangzhou 310053 P. R. China＂ College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 P. R. China Department of Clinical Pharmacology Guangdong Provincial Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Traditional Chinese Medicine Guangzhou 510006 P. R. China

In order to identify the potential nephrotoxic compounds in traditional Chinese medicine lithospermum erythrorhizon,it was separated into serial fractions according to their *** in vitro method was utilized to determine the nephrotoxicity of these fractions with the help of fluorescence image *** a result,the primary fraction A05 and its secondary fractions C06 ＂C09 and C12 ＂C14 were found to have significant toxicity to LLC-PK1 cell line,as determined by the survive rate less than 20% after they were treated with these *** potential nephrotoxic fractions were further analyzed by multistage and high resolution mass *** main compounds in these fractions were tentatively identified to be acetylshikonin,isobutyrylshikonin,β,β＇-dimethyla-cryloylshikonin,and isovalerylshikonin,which may bring nephrotoxicity.

关键词： Traditional Chinese medicine Nephrotoxic compound Liquid chromatography/ion-trap mass spectrome-try(LC/IT-MS) Liquid chromatography/time-of-flight mass spectrometry(LC/TOF-MS) lithospermum erythrorhizon

Activation of GSDME by lithospermum erythrorhizon drives pyroptotic cell death

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Journal of Traditional Chinese Medical Sciences 2023年第3期10卷 280-288页

作者： Sanjiao Wang Zhaoheng Liu Jiajian Guo Yunqian Li Yue Zhang Mingrui Yang Guoqi Niu Wenfu Ma School of Life Science Beijing University of Chinese MedicineBeijing 102488China Structural Biology Program Memorial Sloan Kettering Cancer CenterNew York 10065USA

Objective:To reveal GSDME-executed pyroptosis in cancer cells induced by the Chinese traditional herbal medicine plant lithospermum erythrorhizon(***,Zi Cao)and to investigate the potential ***:*** was extracted by ultrasonication in 95%ethanol,and determined using high-performance liquid chromatography(HPLC).He La,A549,SW620,HEK-293 T,THP-1,K562,Raw264.7 and MDA-MB-231 cell lines were used to investigate the morphology and mechanism of pyroptosis induced by *** lactate dehydrogenase(LDH)release,propidium iodide(PI)/Hoechst double-staining,and pyroptosis reconstitution experiments were performed to study ***-induced cell ***:Compared with the death inhibitor,PI/Hoechst and LDH release experiments,we found that *** induced *** and western blot experiments confired that *** induced GSDME cleavage,which drives *** phenomenon is conserved in several cancer cell lines that might be triggered by caspase family *** mechanism of *** inducing pyroptosis is widely found in tumor ***:Our findings not only explain how *** triggers cancer cell pyroptosis,but also provide mechanistic insights to guide its clinical application in the future.

关键词： lithospermum erythrorhizon Pyroptosis GSDME Anti-tumor activity

On-line HPLC-DAD coupled with ESI-IT-TOF-MS and fluorescence detection to identify DNA-binding compounds from lithospermum erythrorhizon using acridine orange as the fluorescence probe

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Journal of Chinese Pharmaceutical Sciences 2015年第9期24卷 581-590页

作者：黄文芳张藏蔓李森森梁毅王弘陈世忠北京大学医学部药学院天然药物学系北京100191

We have developed an on-line detection method using acridine orange as the fluorescence probe and applied this method to rapidly identify active compounds in herbal medicines. This on-line method was equipped with a high-performance liquid chromatography tandem diode array detector, electrospray ionization-ion-trap time-of-flight mass spectrometry and DNA- acridine orange fluorescence detection （HPLC-DAD-MSn-DNA-AO-FLD）. A large amount of information could be simultaneously obtained during one run, which included HPLC fingerprint, ultraviolet spectra, total ion chromatograms, MSn data of high-resolution mass spectrometry and activity profile of each compound binding with DNA. The method also provided information on structureactivity relationships and mechanism of interaction. We used this on-line method to identify five DNA-binding activity components from lithospermum erythrorhizon sample for the first time. The result showed that the parent nucleus of shikonin derivatives could bind with DNA. The structure-activity relationship showed that the parent nucleus of shikonin derivatives plays a major role in DNA binding, not the carboxyl group on the side chain. This simple, rapid, high precision and good stability on-line method should be useful for compound separation, structural identification and screening of DNA-binding compounds in herbal medicines.

关键词： On-line lithospermum erythrorhizon Acridine orange DNA DNA-binding activity

Endogenous Jasmonic acid Regulates Shikonin Biosynthesis in lithospermum erythrorhizon Cell Cultures

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Endogenous Jasmonic acid Regulates Shikonin Biosynthesis in ...

Orthogonal Experiment Design to Optimize the Pet Dog Dyeing Condition of Musa basjoo Siebold(MBS) & lithospermum erythrorhizon(LE) Mixed Hair Dye

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Lingling Wang, Shaohua Liu, Jinliang Qi, Yanjun Pang, Hengguan Shen, Yonghua Yang* Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, PR China

＜正＞In this study, our results revealed that endogenous jasmonic acid （JA） play a crucial role in triggering the formation of these metabolites in lithospermum erythrorhizon cell cultures during the process of med... 详细信息

关键词： lithospermum erythrorhizon Shikonin Jasmonic acid Nitric oxide

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Medicinal Plant 2018年第4期9卷 114-118页

作者： Jiaqi LI Juan LIU College of Animal Science Southwest University Laboratory of Veterinary Medicine Innovation Research Center in Chongqing Veterinary Engineering Technology Research Center

[Objectives] To optimize the pet dog dyeing process of plant hair dye. [Methods]Three factors affecting dyeing were selected including pH,concentration of dye and dyeing time. The L_9(3~3) orthogonal design was used to evaluate the hair coloring effect and the absorbance of cleaning solution after hair was dyed. [Results] The optimum pet dog dyeing process of plant hair dye was dye concentration of50 mg/mL,dye pH of 7. 5 and dyeing time of 1 h. [Conclusions] The coloring and fixing effects of the dye and dyeing process are good,which can lay a foundation for the development and utilization of plant dyes.

关键词： Dyeing hair Plant dye Pet dog Musa basjoo Siebold lithospermum erythrorhizon Orthogonal test

Studies on Chemical Constituents ofL. erythrorhizon

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Chemical Research in Chinese Universities 1994年第3期10卷 263-265页

作者： BAI Guang and JIN Xiang-jun(Department of Chemistry,Jilin University, Changchun. 130023 )

ＳｔｕｄｉｅｓｏｎＣｈｅｍｉｃａｌＣｏｎｓｔｉｔｕｅｎｔｓｏｆＬ．ＥｒｙｔｈｒｏｒｈｉｚｏｎＢＡＩＧｕａｎｇａｎｄＪＩＮＸｉａｎｇ－ｊｕｎ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＪｉｌｉｎＵｎｉｖｅｒｓｉｔｙ，... 详细信息

ＳｔｕｄｉｅｓｏｎＣｈｅｍｉｃａｌＣｏｎｓｔｉｔｕｅｎｔｓｏｆＬ．ＥｒｙｔｈｒｏｒｈｉｚｏｎＢＡＩＧｕａｎｇａｎｄＪＩＮＸｉａｎｇ－ｊｕｎ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＪｉｌｉｎＵｎｉｖｅｒｓｉｔｙ，Ｃｈａｎｇｃｈｕｎ．１３００２３）...

关键词： lithospermum erythrorhizon Naphthoquinone,Constituent