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Vaginal Intraepithelial Neoplasia Detected with Cervical liquid-based Cytology: Old Concerns or New Facilities?

Open Journal of Obstetrics and Gynecology 2015年第11期5卷 580-587页

作者： Mariana Camezim Beldi Maricy Tacla Marcia Farina Kamilos Fabiane Sarmanho Alexandre Ab’Saber Sheila Siqueira Venancio Avancini Ferreira Alves Edmundo C. Baracat Adhemar Longatto-Filho Hospital das Clinicas of Faculty of Medicine Sao Paulo State University Sao Paulo Brazil

Background: The detection of vaginal intraepithelial neoplasia (VAIN) in cervical samples is not a common finding. Therefore, we aimed to report VAINs detected in liquid-based cytology (LBC) from women examined at Hospital das Clínicas of Faculty of Medicine, Sao Paulo State University. Materials and Methods: We evaluated LBC samples from women referred to gynecology examination for different reasons (previous abnormal PapTest, follow up of treated cervical lesion, ecc) and women examined for regular screening proposals, and compared with biopsy diagnoses, including the controversial diagnoses of vaginal intraepithelial neoplasia (VAIN). Results: From 1866 patients, 1423 (76.3%) cases were negative and 443 (23.7%) were positive for any cellular alteration. Age of patients ranged from 12 to 86 years. We detected 25 histologically confirmed VAIN (1.3% p = 0.0002 by Fisher’s exact test IC 95% 0.0090 - 0.0198) and 1.1% VAIN (p = 0.0031 by Fisher’s exact test IC 95% 0.0077 - 0.0179). Conclusion: The identification of VAIN in routine is feasible;the professionals involved with cytological examination should be aware of these lesions in Pap test samples.

关键词： liquid-based Cytology Cervical Screening VAIN HPV Induced Lesions Vaginal

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How liquid based Microbiology Can Change the Workflow in the Microbiology Laboratories

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Advances in Microbiology 2013年第6期3卷 504-510页

作者： Carla Fontana Marco Favaro Cartesio Favalli Department of Experimental Medicine and Surgery “Tor Vergata” University of Rome Rome Italy

liquid-based microbiology (LBM) is the future for the technological development of microbiology laboratories. In particular, the Eswab system (by Copan) simplifies and streamlines specimen collection and represents the only liquid system supporting the recovery of all types of bacteria (aerobic, anaerobic, and fastidious bacteria). In addition, LBM offers advantages in the efficiency of microorganism recovery and ease of sampling, transport, and storage. LBM also allows the introduction of true automation in the laboratory: either by using Copan ? (Walk-Away Specimen Processor) or any other commercially available specimen processor that utilizes LBM. In this paper, we illustrate how LBM can positively change laboratory workflow by illustrating several years of our experience with LBM. LBM allows clinical specimen optimization and has several important advantages: cost reduction (due to the smaller number of different devices used), time savings for medical or nursing staff (less confusion in collection device selection and fewer samples being collected), time savings for laboratory staff (fewer samples to access and handle for individual investigations), and patient comfort improvement (multiple sample collection can be avoided). A unique collection device for several investigations also guarantees quality due to the uniformity of the sample and standardization of procedures.

关键词： Workflow liquid-based Microbiology Specimen Processor Molecular Assays Rational Use of Laboratory Resources

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