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Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method

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Journal of Pharmaceutical Analysis 2018年第4期8卷 240-249页

作者： Xing-Xin Yang Yu-Zhen Zhou Feng Xu Jie Yu Gegentana Ming-Ying Shang Xuan Wang Shao-Qing Cai Department of Natural Medicines School of Pharmaceutical Sciences Peking University Department of Chemical Biology School of Pharmaceutical Sciences Peking University College of Pharmaceutical Science Yunnan University of Traditional Chinese Medicine

Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry（LC/MS） method,also known as screening method for mitochondria-targeted bioactive constituents（SM-MBC）. This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines（TCMs）, including Puerariae Radix（PR） and Chuanxiong Radix（CR）. A total of 23 active compounds were successfully discovered from the two TCMs extracts. Among these 23 hit compounds, 17 were identified by LC/MS, 12 of which were novel mitochondria-targeting compounds. Among these, 6 active compounds were analyzed in vitro for pharmacological tests and found able to affect mitochondrial functions. We also investigated the effects of the hit compounds on Hep G2 cell proliferation and on loss of cardiomyocyte viability induced by hypoxia/reoxygenation injury. The results obtained are useful for in-depth understanding of mechanisms underlying TCMs therapeutic effects at mitochondria level and for developing novel potential drugs using TCMs as lead compounds. Finally, we showed that SM-MBC was an efficient protocol for the rapid screening of mitochondria-targeting constituents from complex samples such as PR and CR extracts.

关键词： Mitochondria Bioactive constituents Traditional Chinese medicines Centrifugal ultrafiltration liquid chromatography/mass spectrometry

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Phytochemical constituents,antioxidant and antibacterial activities of methanolic extract of Ardisia elliptica

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Asian Pacific Journal of Tropical Biomedicine 2017年第6期7卷 569-576页

作者： Nazeh M.Al-Abd Zurainee Mohamed Nor Marzida Mansor Asdren Zajmi Mohd Shahnaz Hasan Fadzly Azhar Mustafa Kassim Department of Parasitology Faculty of MedicineUniversity of Malaya Faculty of Medicine and Health Sciences University of Aden Department of Anesthesiology Faculty of MedicineUniversity of Malaya Department of Diagnostic and Allied Health Sciences Faculty of Health and Life SciencesManagement and Science University Department of Chemistry Faculty of ScienceUniversity of Malaya

Objective:To investigate the antioxidant,antibacterial,and chemical ingredients of Ardisia elliptica(***) methanolic ***:The plant was extracted using *** and antioxidant activities were ***:The results showed that both fruit and leaf extract of *** have significant antibacterial activities against Gram-positive and Gram-negative *** extracts showed higher content of phenolic(71 ± 0.03 GAE/mg extract dry weight),in comparison to the leaf extracts(37 ± 0.05 GAE/mg extract dry weight).Flavonoid content,and Fe2+chelating activity of fruit extracts were higher than leaf *** percentage radical inhibition of fruit extract is found to be higher(70%) than that of leaf extract(60%).LCMS results indicated that the major compounds in the fruit extract were Gingerol,Aspidin,Kampherol,and Stercuresin,while the leaf extract contained Gingerol,Aspidin,Triangularin,and Salicyl acyl ***,the results of GCMS indicated that fruit extract contained these major compounds:Vitamin E Tocopherol,5-hepylresornicol,2-Nonylmalonic acid,5-pentadecylresornicol,and ***,leaf extract of *** contained these major compounds:Alpha Amyrenol,4,4,6,6a,6b,8,8a,9,10,11,12,12 a,14,14 a,14 b octadehydro-2H-picen-3-one,and Lonasterol,4-t-Butyl-2-[4-nitrophenyl] ***:The results provide evidence that fruit and leaf of *** extracts might indeed be used as a potential source of effective natural antimicrobial and antioxidant agents in pharmaceutical and food industries.

关键词： Ardisia elliptica Antioxidant capacity Total phenol content Gas chromatography/mass spectrometry liquid chromatography/mass spectrometry

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