检索结果-南通市图书馆

Water Biology and Security 2022年第2期1卷 105-111页

作者： Hexiong Feng Xu-Fang Liang College of Fisheries and Chinese Perch Research Center Huazhong Agricultural UniversityWuhan430070China Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt Ministry of EducationWuhan430070China

In mammals,lipoprotein lipase(LPL)has been found to play an important role in lipid mentalismand *** deficiency in humans(Homo sapiens)and mice(Mus musculus)tends to cause *** lpl gene is not expressed in adult mammalian liver,but is in adult fish *** functions provided by the lpl gene are diverse in ***,we knocked out the lpl gene in Japanese medaka(Oryzias latipes)with the CRISPR/Cas9 *** lpl-knockout(KO)homozygous individuals showed severe developmental defects with an extremely emaciated and deformedbody and onlyaccounted for about5%of the *** is consistentwiththefindings inmicebut disaccords with the results for zebrafish(Danio rerio).Compared with wild-type(WT)madaka,the mRNA level of lpl in lpl-KO heterozygous mutant was significantly higher in the muscle,showed no significant difference in the liver,and was significantly lower in the *** lpl heterozygous deficiency,the relative area of Oil RedO and triglycerides(TG)level in the liver,heart and muscle tissue covaried with levels of lpl mRNA in *** lpl heterozygous deficiency did not affect the levelsofTG,low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)and total cholesterol(TC)in the plasma of medaka,which is inconsistent with the findings in *** general,the lpl gene plays an important role in the growth and development and is closely related to lipid deposition of medaka.

关键词： lipoprotein lipase Knockout Lipid Japanese medaka(Oryzias latipes)

Establishment of Real-Time TaqMan-Fluorescence Quantitative RT-PCR Assay for Detection and Quantification of Porcine lipoprotein lipase mRNA

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2009年第10期8卷 1256-1262页

作者： LIAN Hong-xia LU De-xun GAO Min College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450002 P.R. China Institute of Animal Nutrition Inner Mongolian Academy of Agricultural and Animal Sciences Huhhot 010031 P.R. China

Porcine lipoprotein lipase （LPL） cDNA was cloned as the standard for real-time quantifying LPL mRNA and the TaqMan-fluorescence quantitative PCR assay for detection was established. The total RNA extracted from Longissimus dorsi of porcine was reverse-transcribed to cDNA. LPL cDNA was ligated with pGM-T vector and transformed into Escherichia coli TOP10. Plasmid DNA extracted from positive clones was verified by PCR amplification and sequenced. LPL was amplified by real-time fluorescence quantitative PCR from the plasmid DNA. The concentration of DNA template purified was detected by analyzing absorbance in 260 nm and then the combined plasmid was diluted to series as standard for fluorescence quantitative PCR （FQ-PCR）. The method of LPL mRNA real-time PCR was well established, which detected as low as 103 with the linear range 10^3 to 10^10 copies. The standard curves showed high correlations （R2 = 0.9871）. A series of standards for real-time PCR analysis have been constructed successfially, and real-time TaqMan-fluorescence quantitative RT-PCR is reliable to quantitatively evaluate FQ-PCR mRNA in L. dorsi of porcine.

关键词： porcine lipoprotein lipase FQ-PCR TaqMan fluorogenic probe

Hypotriglyceridemic Effects of Apple Polyphenols Extract via Up-Regulation of lipoprotein lipase in Triton WR-1339-Induced Mice

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2014年第1期20卷 31-35页

作者：姚楠何蓉蓉曾晓会黄雪君杜铁良崔景朝栗原博 Guangdong Provincial Institute of Traditional Chinese Medicine Institute of Traditional Chinese Medicine and Natural Products Jinan University

Objective：To investigate the anti-hyperlipidemic effects of apple polyphenols extract（APE）in Triton WR-1339-induced endogenous hyperlipidemic ***：Firstly,APE was isolated and purified from the pomace of Red Fuji Apple and contents of individual polyphenols in APE were determined using highperformance liquid chromatography-mass spectrometry（HPLC-MS）.Secondly,forty male National Institude of Health（NIH）mice were randomly divided into 5 groups with 8 animals in each *** Fenofibrate Capsules（FC）group and APE groups received oral administration of respective drugs for 7 consecutive *** mice except those in the normal group were intravenously injected through tail vein with Triton WR-1339 on the6th *** and livers from all the mice were obtained 18 h after the *** changes in serum total cholesterol（TC）,triglyceride（TG）,lipoprotein lipase（LPL）and hepatic triglyceride lipase（HTGL）were measured by respective ***,expression of hepatic peroxisome proliferator-activated receptor alpha（PPARα）mRNA was measured by real-time reverse transcription-polymerase chain reaction（RT-PCR）***：Serum TC and TG levels significantly increased in Triton WR-1339-induced model group compared with the normal group（P〈0.01）.Oral administration of APE[200 and 400 mg/（kg·day）]dose-dependently reduced the serum level of TG in hyperlipidemic mice（P〈0.01）.Serum LPL and HTGL activities significantly decreased in Triton WR-1339-induced model group compared with the normal group（P〈0.05）.Oral administration of APE[200 and 400 mg/（kg·day）]dose-dependently elevated the serum activity of LPL in hyperlipidemic mice（P〈0.05or P〈0.01）.Furthermore,compared with the normal group,hepatic mRNA level of PPARαin the model group significantly decreased（P〈0.01）.Oral administration of APE[200 and 400 mg/（kg·day）]dose-dependently elevated the expression of PPARαin hyperlipidemic mice（P〈0.05 or P〈0.01）.Conclusion：APE could reduce TG level via up-regulation of LPL activity,

关键词： apple polyphenols extract Triton WR-1339 triglyceride lipoprotein lipase peroxisome proliferator-activated receptor alpha

lipoprotein lipase Ser447Stop and Hind Ⅲ polymorphisms differently modify effects of high carbohydrate diet on serum lipidprofile in a gender-specific manner

在线全文

学校读者我要写书评

暂无评论

Lipoprotein lipase Ser447Stop and Hind Ⅲ polymorphisms diff...

Effect of Astragalus-Angelica Mixture on lipoprotein lipase and Lecithin-Cholesterol Acyltransferase of Nephrotic Rats

第九届西南三省一市生物化学与分子生物学学术交流会

作者： Xin Huang,MS Ren Rong Gong,RN Zhengke Li,MD Hui Tang,MD Jia Lin,MD Chanfen Gan,MD Ronghui Li,MS Li Ying Xiao,MD Wei Duan,P_HD and Ding Zhi Fang,MD P_HD *Department of Biochemistry and Molecular Biology,and State Key Laboratory of Oral Diseases,West China Medical Center,Sichuan University,Chengdu 610041 P.R.China Department of Cardiac and Thoracic Surgery,West China Hospital,Sichuan University,Chengdu 610041 P.R.China School of Medicine,Deakin University,Geelong Victoria 3217 Australia)

Objectives:To test effects of interactions between LPL polymorphisms and high carbohydrate (HC) diet on serum lipid profile in a young Chinese population well characterized with a lower incidence of coronary artery di... 详细信息

关键词： hypertriacylglycerolemia lipoprotein lipase high carbohydrate diet gene polymorphism Ser447Stop Hind Ⅲ insulin

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2000年第1期6卷 35-37页

作者：李宁军李惊子辛岗许远洪健美王海燕

Objective: To investigate the mechanism of Astragalus-Angelica Mixture (AAM) ’s effects on lipid metabolism disturbance in nephrotic ***: To examine the effects of AAM on serum albumin, lipid levels, and activities of lipoprotein lipase (LPL) and lecithin-cholesterol acyltransferase (LCAT), which are key enzymes for metabolism of lipid in immune-induced nephrotic hyperlipidemia rats and exogenous hyperlipidemia ***: In nephrotic rats, serum albumin was reduced, lipid increased significantly, LPL activity decreased markedly and the LCAT activity was relatively insufficient. Activities of LPL and LCAT increased obviously in AAM treated nephrotic rats. There were no change of activities of LPL and LCAT in exogenous hyperlipidemia rats and AAM showed no effect on the activities of these two ***: The effect of AAM on regulating lipid metabolism might be due to enhance the clearance of both triglyceride-rich and cholesterol-rich apoB containing lipoprotein by improving the activities of LPL and LCAT.

关键词： Astragalus-Angelica Mixture nephrotic syndrome lipoprotein lipase lecithin-cholesterol acyltransferase hyperlipidemia

Effect of lipoprotein lipase Gene Polymorphism on Plasma Lipid Levels, BMI and Subcutaneous Fat Distribution in Simple Obesity Children

在线全文

学校读者我要写书评

暂无评论

Journal of Nanjing Medical University 2000年第2期14卷 86-90页

作者：刘倩琦郭锡熔陈荣华莫宝庆 Institute of Pediatrics Nanjing Medical University Nanjing 210029 P. R. China

Objective To study the effects of Hind Ⅲ DNA polymorphism in the lipoprotein lipase (LPL)gene on plasma lipid levels, body mass index (BMI) and subcutaneous fat distribution in simple obesity *** The LPL Hind Ⅲ genotypes were detected with the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques in 92 children with simple obesity. The levels of the plasma lipid, plasma lipoproteins, BMI and skinfold thickness in three regions (biceps, subscapular and abdominal wall) were also measured. Results It was shown that the levels of TG, TC, LDL C, ApoB, BMI, biceps and subscapular skinfold thickness, and the average value of the skinfold thickness in three regions were significantly higher in the obese children with H+H+ genotype than those with H+H-genotype. Conclusions It can be concluded that LPL Hind Ⅲ polymorphism may modify the levels of plasma lipid, plasma lipoprotein and BMI in children with simple obesity, and meanwhile alters the distribution of subcutaneous fat.

关键词： lipoprotein lipase polymorphism obesity children

Implication of lipoprotein lipase-deficient impaired learning and memory:focus on aging and testosterone deficiency

在线全文

学校读者我要写书评

暂无评论

Implication of lipoprotein lipase-deficient impaired learnin...

The interplay between non-esterified fatty acids and bovine peroxisome proliferator-activated receptors: results of an in vitro hybrid approach

2019全国老年痴呆与认知障碍相关疾病学术大会

作者： ZHAO Lei YE Zhan-lei JIN Ying-lan ZHANG Bo CUI De-hua HAN Hong-bin Department of Pathology MRIPeking University HSCThird Hospital

OBJECTIVE Normal male aging is associated with declines in levels of the sex steroid hormone testosterone.A large body of evidence supports a neurotrophic role for testosterone in central nervous *** lipase(LPL) is also expressed in the brain with highest levels found in the pyramidal cells of the hippocampus,we previous reported LPL-deficient mice exhibited memory *** is known to be largely converted to estradiol following aromatization within the *** testosterone has been implicated in lipid metabolism,it remains elusive whether testosterone can regulate brain LPL through DNA methylation *** order to clarify DNA methylation control exerted by testosterone over LPL gene in central nervous system,and its effect on lipid metabolism,we examined the adult male rat hippocampus to determine whether castration induced testosterone deficiency can affect lipid profile and LPL gene expression through its altered methylation *** Model of aging with declines in levels of the sex steroid hormone testosterone was performed as our previous *** ① Serum testosterone and brain testosterone levels were significantly decreased,which were restored to the control level after testosterone replacement,respectively(P<0.01);② Androgen deficiency was not found in Morris water maze and motor performance,however,androgen deficiency increases neurological and cognitive impairment in aged rats.③Decreased expression of olfactory marker protein(OMP) in olfactory bulb of SD rats treated with androgen deficiency.④ The expression of FoxO3 and OMP in the olfactory bulb of androgen deficient rats was down-regulated,accompanied by dysfunction of the olfactory limbic system.⑤ Decreased LPL mRNA level and inversely increased LPL promoter methylation level were observed following androgen deficiency and reserved by testosterone replacement.⑥ In contrast,androgen deficiency slightly increased estrogen receptor beta(ERβ

关键词： lipoprotein lipase sex steroid hormones DNA methylation homeostatic regulation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2021年第1期12卷 292-304页

作者： Sebastiano Busato Massimo Bionaz Department of Animal and Rangeland Sciences Oregon State UniversityCorvallisORUSA

Background: In dairy cows circulating non-esterified fatty acids(NEFA) increase early post-partum while liver and other tissues undergo adaptation to greater lipid metabolism, mainly regulated by peroxisome proliferator-activated receptors(PPAR). PPAR are activated by fatty acids(FA), but it remains to be demonstrated that circulating NEFA or dietary FA activate bovine PPAR. We hypothesized that circulating NEFA and dietary FA activate PPAR in dairy ***: The dose-response activation of PPAR by NEFA or dietary FA was assessed using HP300 e digital dispenser and luciferase reporter in several bovine cell types. Cells were treated with blood plasma isolated from Jersey cows before and after parturition, NEFA isolated from the blood plasma, FA released from lipoproteins using milk lipoprotein lipase(LPL), and palmitic acid(C16:0). Effect on each PPAR isotype was assessed using specific synthetic ***: NEFA isolated from blood serum activate PPAR linearly up to ~ 4-fold at 400 μmol/L in MAC-T cells but had cytotoxic effect. Addition of albumin to the culture media decreases cytotoxic effects of NEFA but also PPAR activation by ~ 2-fold. Treating cells with serum from peripartum cows reveals that much of the PPAR activation can be explained by the amount of NEFA in the serum(R~2 = 0.91) and that the response to serum NEFA follows a quadratic tendency, with peak activation around 1.4 mmol/L. Analysis of PPAR activation by serum in MAC-T, BFH-12 and BPAEC cells revealed that most of the activation is explained by the activity of PPARδ and PPARγ, but not PPARα. Palmitic acid activated PPAR when added in culture media or blood serum but the activation was limited to PPARδ and PPARα and the response was nil in serum from post-partum cows. The addition of LPL to the serum increased > 1.5-fold PPAR ***: Our results support dose-dependent activation of PPAR by circulating NEFA in bovine, specifically δand γ isotypes. Data also support the

关键词： Albumin Blood serum Bovine Gene reporter Hepatocytes lipoprotein lipase Mammary cells Non-esterified fatty acids Peroxisome proliferator-activated receptor

Effects of CpG-ODN on gene expression in formation of foam cells

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第11期26卷 1359-1364页

作者： Kai LI, Bin WAN, Zhen-lin HU, Ying HE, Xiao-wen HE, Lei JIANG, Shu-han SUN~2 Department of Medical Genetics, College of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China Department of Medical Genetics College of Basic Medical Sciences the Second Military Medical University Shanghai China

Aim: To investigate the effects of CpG-oligodeoxynucleotide (CpG-ODN) on the formation of macrophage foam cells and related gene expression. Methods: A gene expression profile was examined by microarray techniques, and mRNA expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The cholesterol and cholesteryl ester contents of cells were determined by high performance liquid chromatography. Results: CD36, LPL, and Fcγ2b, which were related to lipid metabolism and the formation of macrophage foam cells, were upregulated after CpG-ODN stimulation. The mRNA expression related to the formation of foam cells was confirmed by semiquantitative RT-PCR. Moreover, histochemical analysis confirmed that lipid deposits inside cells increased after CpG-ODN treatment. However, using flow cytometry, we found that CpG-ODN had no effect on the expression of membrane receptors. Conclusion: CpG-ODN up-regulated the expression of genes in macrophage foam cell formation.

关键词： CpG islands oligodeoxyribonucleotides foam cells macrophages LDL lipoprotein gene expression cholesterol CD 36 antigens lipoprotein lipase Fc gamma receptor Ⅱb

Effects of dietary sodium butyrate on growth,digestive enzymes,body composition and nutrient retention-related gene expression of juvenile yellow catfish (Pelteobagrus fulvidraco)

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2021年第2期7卷 539-547页

作者： Hongxia Zhao Guoxia Wang Hairui Wang Wenyan Mo Yanhua Huang Junming Cao Peijia Li Institute of Animal Science Guangdong Academy of Agricultural SciencesGuangzhou 510640China Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture and Rural AffairsGuangzhou 510640China Guangdong Key Laboratory of Animal Breeding and Nutrition Guangzhou 510640China Guangdong Ocean University Zhanjiang 524088China

An 8-week feeding trial was conducted to evaluate the effects of sodium butyrate(SB)on growth,digestive enzymes,body composition and nutrient retention-related gene expression of juvenile yellow catfish(Pel-teobagrus fulvidraco).Five isonitmgenous and isolipidic diets(420 g/kg protein and 90 g/kg lipid)were formulated to contain 0(control),250,500,1,000 or 2,000 mg/kg *** groups of 40 fish(BW=1.26±0.01 g)per tank(300-L cylindrical fiberglass tanks)for each diet were fed to apparent satiation twice ***,hepatopancreas and intestine samples were obtained for digestive enzymes activities analyses.A real-time quantitative PCR analysis was performed to determine the relative expression of target of rapa-mycin(TOR)and lipoprotein lipase(LPL)in the hepatopancreas and *** fed the diets supplemented with SB at 500 and 1,000 mg/kg showed significantly higher specific growth rate and significantly lower feed conversion ratio compared to the control(Plipase,intestinal lipase,alkaline phosphatase and y-glutamyl trans-peptidase for fish fed 1,000 mg/kg SB compared to the control(P<0.05).Intestine length index,intestine somatic index,fold height and muscular thickness of distal intestine were significantly higher in 1,000 mg/kg SB groups compared to the control(P<0.05).Significantly higher levels of whole-body crude protein,ash,calcium,phosphorus,nutrition retention and relative mRNA of intestinal TOR were observed in 1,000 mg/kg SB group(P<0.05).Whole-body lipid content and hepatopancreas LPL mRNA expression in 2,000 mg/kg SB gmup were significantly higher than the control(P<0.05).Relative mRNA levels of intestinal LPL and hepatopancreas TOR were significantly higher in the 500 mg/kg SB group compared to those in other groups(P<0.05).The increased

关键词： Sodium butyrate Growth Digestion Absorption Target of rapamycin lipoprotein lipase