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Salt Stress in Arabidopsis： lipid transfer protein AZI1 and Its Control by Mitogen-Activated protein Kinase MPK3

Molecular Plant 2014年第4期7卷 722-738页

作者： Andrea Pitzschke Sneha Datta Helene Persak Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Muthgasse 18 1190 Vienna Austria

A plant＇s capability to cope with environmental challenges largely relies on signal transmission through mitogen-activated protein kinase （MAPK） cascades. In Arabidopsis thaliana, MPK3 is particularly strongly associated with numerous abiotic and biotic stress responses. Identification of MPK3 substrates is a milestone towards improving stress resistance in plants. Here, we characterize AZI1, a lipid transfer protein （LTP）-related hybrid proline-rich protein （HyPRP）, as a novel target of MPK3. AZI1 is phosphorylated by MPK3 in vitro. As documented by co-immunoprecipitation and bimolecular fluorescence complementation experiments, AZI1 interacts with MPK3 to form protein complexes in planta. Furthermore, null mutants of azil are hypersensitive to salt stress, while AZIl-overexpressing lines are markedly more tolerant. AZI1 overexpression in the mpk3 genetic background partially alleviates the salt-hypersensitive phenotype of this mutant, but functional MPK3 appears to be required for the full extent of AZIl-conferred robustness. Notably, this robustness does not come at the expense of normal development. Immunoblot and RT-PCR data point to a role of MPK3 as positive regulator of AZI1 abundance.

关键词： salt stress Arabidopsis MAPK MPK3 lipid transfer protein AZI1 phosphorylation in vivo interaction.

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OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice

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Journal of Genetics and Genomics 2022年第5期49卷 481-491页

作者： Libin Chen Chonghui Ji Degui Zhou Xin Gou Jianian Tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen Yongyao Xie State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Laboratory for Lingnan Modern Agriculture GuangzhouGuangdong 510642China Guangdong Key Laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China Present address:Vector-borne Virus Research Center Fujian Province Key Laboratory of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China.

In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall development.However,the functional cooperation of different LTPs in pollen wall development is not well understood.In this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding activity.Dysfunction of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male sterility.Yeast two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os C6.These findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.

关键词： Rice Pollen wall lipid transfer protein Male sterility

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BcLTP,a Novel lipid transfer protein in Brassica chinensis,may Secrete and Combine Extracellular CaM

BcLTP,a Novel Lipid Transfer Protein in Brassica chinensis,m...

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中国细胞生物学学会第九次会员代表大会暨青年学术大会

作者： Chun-Ming Wang~1 Wan-Qin Xie~2 Cui-Feng Li~2 Ying Sun~1 1 Institute of Molecular Cell Biology,Hebei Normal University,Shijiazhuang 050016,China 2 College of Life Science,Nankai University,Tianjin 300071,China

lipid transfer proteins in plants are believed to be involved in many processes of cell physiology and development. In this work,a full length cDNA encoding a novel lipid transfer protein,designated BcLTP was isolated from Brassica chinensis.At least two copies of BcLTP are present in whole genome of Brassica chinensis,and its tran-

关键词： lipid transfer protein CaM isoforms extracellular space

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Plasma phospholipid transfer protein （PLTP） modulates adaptive immune functions through alternation of T helper cell polarization

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Cellular & Molecular Immunology 2016年第6期13卷 795-804页

作者： Catherine Desrumau Stephanie Lemaire-Ewing Nicolas Ogier Akadiri Yessoufou Arlette Hammann Anabelle Sequeira-Le Grand Valerie Deckert Jean-Paul Pais de Barros Naig Le Guern Julien Guy Naim A Khan Laurent Lagrost INSERM UMR866 - F21079 Dijon France Univ. Bourgogne Franche-Comte F-21000 Dijon France LipSTIC LabEx Fondation de Cooperation Scientifique Bourgogne-Franche Comte F-21000 Dijon France CHU Hopital du Bocage Dijon France plateforme de Cytometrie en Flux Universite de Bourgogne Dijon France

Objective： Plasma phospholipid transfer protein （PLTP） is a key determinant of lipoprotein metabolism, and both animal and human studies converge to indicate that PLTP promotes atherogenesis and its thromboembolic complications. Moreover, it has recently been reported that PLTP modulates inflammation and immune responses. Although earlier studies from our group demonstrated that PLTP can modify macrophage activation, the implication of PLTP in the modulation of T-cell-mediated immune responses has never been investigated and was therefore addressed in the present study. Approach and results： In the present study, we demonstrated that PLTP deficiency in mice has a profound effect on CD4＋ ThO cell polarization, with a shift towards the anti-inflammatory Th2 phenotype under both normal and pathological conditions. In a model of contact hypersensitivity, a significantly impaired response to skin sensitization with the hapten-2,4-dinitrofluorobenzene （DNFB） was observed in PLTP-deficient mice compared to wild-type （WT） mice. Interestingly, PLTP deficiency in mice exerted no effect on the counts of total white blood cells, lymphocytes, granulocytes, or monocytes in the peripheral blood. Moreover, PLTP deficiency did not modify the amounts of CD4＋ and CD8＋ T lymphocyte subsets. However, PLTP-deficiency, associated with upregulation of the Th2 phenotype, was accompanied by a significant decrease in the production of the pro-Thl cytokine interleukin 18 by accessory cells. Conclusions： For the first time, this work reports a physiological role for PLTP in the polarization of CD4＋ T cells toward the pro-inflammatory Th I phenotype.

关键词： lipid transfer protein lymphocyte polarization T cell

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Analysis of Five Differentially Expressed Gene Families in Fast Elongating Cotton Fiber

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 51-56页

作者： Jian-Xun FENG, Sheng-Jian JI, Yong-Hui SHI, Yu XU, Gang WEI, and Yu-Xian ZHU( National Laboratory of protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China ) National Laboratory of protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

Using the suppression subtractive hybridization method, we isolated five gene families, including proline-rich proteins (PRPs), arabinogalactan proteins (AGPs), expansins, tubulins and lipid transfer proteins (LTPs), from fast elongating cotton fiber cells. Expression profile analysis using cDNA array technology showed that most of these gene families were highly expressed during early cotton fiber developmental stages (0-20 days post anthesis, DPA). Many transcripts accumulated over 50-fold in 10 DPA fiber cells than in 0 DPA samples. The entire gene family -AGP, together with 20 individual members in other 4 gene families, are reported in cotton for the first time. Accumulation of cell wall proteins, wall loosening enzymes, microtubules and lipid transfer proteins may contribute directly to the elongation and development of fiber cells.

关键词： cotton fiber cDNA array cell elongation arabinogalactan protein lipid transfer protein

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Characterization and genetic mapping of a Photoperiod Sensitive Dwarf 1 locus in rice(Oryza sativa L.)

Characterization and genetic mapping of a Photoperiod Sensit...

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第十四届全国植物基因组学大会

作者： Riqing Li Jixing Xia Xiucai Zhao Yiwei Xu Yaoguang Liu Yuanling Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education InstitutionsCollege of Life SciencesSouth China Agricultural University

Plant height is an important agronomic trait for crop architecture and yield.Most known factors determining plant height function in gibberellin or brassinosteroid biosynthesis or signal transduction.Here,we report a ... 详细信息

关键词： plant height lipid transfer protein dwarf photoperiod-sensitive rice

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