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Changes in Thermostability of Photosystem Ⅱ and Leaf lipid composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes

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Journal of Integrative Plant Biology 2007年第4期49卷 515-522页

作者： Yunlai Tang Mei Chen Yinong Xu Tingyun Kuang College of Life Science and Engineering Southwest University of Science and Technology Mianyang 621000 China Photosynthesis Research Center Institute of Botany the Chinese Academy of Sciences Beijing 100093 China

We studied the difference in thermostability of photosystem Ⅱ （PSII） and leaf lipid composition between a T-DNA insertion mutant rice （Oryza sativa L.） VG28 and its wild type Zhonghuau. Native green gel and SDS-PAGE electrophoreses revealed that the mutant VG28 lacked all light-harvesting chlorophyll a/b protein complexes. Both the mutant and wild type were sensitive to high temperatures, and the maximal efficiency of PSII photochemistry （FJ Fm） and oxygen-evolving activity of PSII in leaves significantly decreased with increasing temperature. However, the PSII activity of the mutant was markedly more sensitive to high temperatures than that of the wild type. lipid composition analysis showed that the mutant had less phosphatidylglycerol and sulfoquinovosyl diacylglycerol compared with the wild type. Fatty acid analysis revealed that the mutant had an obvious decrease in the content of 16：1t and a marked increase in the content of 18：3 compared with the wild type. The effects of lipid composition and unsaturation of membrane lipids on the thermostability of PSII are discussed.

关键词： high temperature lipid composition light-harvesting a/b protein complexes photosystem II rice.

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Effects of different processing methods on the lipid composition of hazelnut oil:a lipidomics analysis

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Food Science and Human Wellness 2022年第2期11卷 427-435页

作者： Jiayang Sun Xiaonuo Feng Chunmao Lyu Shuang Zhou Zixuan Liu College of Food Science Shenyang Agricultural UniversityShenyang 110866China College of Bioscience and Biotechnology Shenyang Agricultural UniversityShenyang 110866China

Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is ***,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing ***,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in *** statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods *** significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing *** results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.

关键词： lipidomics Hazelnut oil Processing method lipid composition

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Development and assessment of tyrosinase inhibitory activity of liposomes of Asparagus racemosus extracts

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Asian Journal of Pharmaceutical Sciences 2013年第2期8卷 134-142页

作者： Narin Therdphapiyanak Montree Jaturanpinyo Neti Waranuch Lalana Kongkaneramit Narong Sarisuta Faculty of Pharmacy Mahidol UniversityBangkokThailand Faculty of Pharmaceutical Sciences Naresuan UniversityPhitsanulokThailand Faculty of Pharmacy Srinakharinwirot UniversityNakhonnayokThailand

The purpose of this study was to develop liposomal formulations of Asparagus racemosus root extract(AR1-6)as well as evaluate the physicochemical properties and in vitro tyrosinase inhibitory *** composed of AR1-6 to lipid weight ratio of 1:10 and lecithin(LEC)or Phospholipon90G(PC90G)as structural phospholipid at 7:3 molar ratio to CHOL were prepared by various methods,***-film(CF),reverse-phase evaporation(REV),polyol dilution(PD),and freeze-drying of monophase solution(MFD)*** results revealed that vesicles prepared by CF and MFD were multilamellar whereas those prepared by REV and PD were oligolamellar in nature with particle sizes ranging from 0.26 to 13.83 *** zeta potentials were in the range of1.5 to39.3 ***1-6 liposomes with LEC possessed significantly higher entrapment than those with *** highest entrapment efficiency and in vitro tyrosinase inhibitory activity of 69.08%and 25%,respectively,were obtained from liposomes having LEC and prepared by PD *** tyrosinase inhibitory activity were in the rank order of LEC>PC90G,and PD>CF>REV>*** could be concluded that the mechanism of vesicle forming in each method of preparation was the key factor influencing physicochemical properties,particularly vesicle type,size,surface charge,and entrapment,which were well correlated with the biological activity.

关键词： Asparagus racemosus Liposomes lipid composition Method of preparation Tyrosinase inhibitory activity

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Total Fat, Fatty Acid composition, Tocopherol and Tocotrienol Profiles in Fermented Beans of Ten Controlled Pollinated Cocoa (Theobroma cacao L.) Hybrids from Cameroon

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American Journal of Plant Sciences 2024年第5期15卷 359-373页

作者： Martin Paul Arnaud Mbida Simon Perrez Akoa Ronelle Fabiola Mbia Manga Ndjaga Jude Martine Louise Ondobo Onomo Pierre Effa Department of Biochemistry Faculty of Science University of Yaounde I Yaounde Cameroon Laboratory of Plant Physiology and Biochemistry Department of Biological Science Higher Teacher&#8217s Training College University of Yaounde I Yaounde Cameroon Department of Food Science and Technology Faculty of Agriculture and Veterinary Medecine University of Buea Buea Cameroon National Technology Development Committee Ministry of Scientific Research and Innovation Administrative Center Yaounde Cameroon Department of Agriculture and Agropastoral Higher Technical Teacher&#8217s Training College University of Ebolowa Ebolowa Cameroon

This study aimed to discriminate ten Cameroonian cocoa hybrids according to their total fat, fatty acid composition, tocopherol and tocotrienol profiles. Six cocoa clones from the gene banks of the Cameroon Cocoa Development Corporation were used to create hybrids. The determination of fatty acid composition was carried out by using a gas chromatography (GC) apparatus coupled by a flame ion detector (FID). Tocopherol and tocotrienol analysis was performed by upper high-performance liquid chromatography (UHPLC). Information on the impact of the genotype on the cocoa fat composition was provided. The major fatty acids (FA) in fermented samples are stearic (34.57%), palmitic (26.13%), oleic (34.13%) and linoleic (3.16%) acids. (35.05% to 35.6%). SCA12 × ICS40, SCA12 × SNK13, SNK13 × T79/501 have the least hard cocoa butters. Tocopherols analysis showed a predominance of γ-tocopherols (94.64 ± 1.51 to 292.16 ± 3.17 µg∙g−1), whereas only a small amount of β and δ-tocopherol (from 0.46 to 2.78 µg∙g−1 and 0.12 to 5.82 respectively) was observed. No γ-tocotrienol was found in fermented samples. A differentiation in terms of total fat and tocopherol content was observed amongst hybrids with the same mother-clone, suggesting an impact of pollen on these compounds.

关键词： Cocoa Hybrids lipid composition UHPLC GC-FID

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