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Chinese Medical Sciences Journal 2012年第1期27卷 18-23页

作者： Yi-jun Zhou Yu-ling Song Hui Zhou Yan Li Department of Endocrinology and Metabolism Fourth Affiliated HospitalChina Medical UniversityShenyang 110034China

Objective To elucidate GPR40/FFA1 and its downstream signaling pathways in regulating insulin secretion. Methods GPR40/FFA1 expression was detected by immunofluorescence imaging. We employed linoleic acid (LA), a free fatty acid that has a high affinity to the rat GPR40, and examined its effect on cytosolic free calcium concentration ([Ca2+]i) in primary rat β-cells by Fluo-3 intensity under confocal microscopy recording. Downregulation of GPR40/FFA1 expression by antisense oligonucleotides was performed in pancreatic β-cells, and insulin secretion was assessed by enzyme-linked immunosorbent assay. Results LA acutely stimulated insulin secretion from primary cultured rat pancreatic islets. LA induced significant increase of [Ca2+]i in the presence of 5.6 mmol/L and 11.1 mmol/L glucose, which was reflected by increased Fluo-3 intensity under confocal microscopy recording. LA-stimulated increase in [Ca2+]i and insulin secretion were blocked by inhibition of GPR40/FFA1 expression in β-cells after GPR40/FFA1-specific antisense treatment. In addition, the inhibition of phospholipase C (PLC) activity by U73122, PLC inhibitor, also markedly inhibited the LA-induced [Ca2+]i increase. Conclusion LA activates GPR40/FFA1 and PLC to stimulate Ca2+ release, resulting in an increase in [Ca2+]i and insulin secretion in rat islet β-cells.

关键词： GPR40 / FFA 1 phospholipase C antisense oligonucleotides intracellular calcium linoleic acid

ELASTICITY OF MONOLAYER OF linoleic acid AND ITS POLYMER

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Chinese Journal of Polymer Science 1998年第2期16卷 147-154页

作者：何平笙 PELTONEN J.P.K. ROSENHOLM J.B. Department of Polymer Science and Engineering University of Science and Technology of China Hefei 230026 China Department of Physical Chemistry Abo Akademi University Abo Finland

The dynamic elasticity of linoleic acid monolayer on a subphase of 10** minus **4mol/L TbCl//3 at various surface pressure has been measured by means of dynamic oscillation method in measuring the change of surface pressure caused by periodic compression-expansion cycles of the barrier. The elasticity of monolayer increases with increasing of surface pressure linearly. The linoleic acid polymer monolayer has been obtained under UV-irradiation in situ when keeping a constant surface pressure. But the elasticity of the resulting polymerized monolayer is even smaller than that of its corresponding monomer monolayer. The elasticity of the polymerized linoleic acid monolayer decreases with increasing polymerization time. The explanation based on entropy has been presented. (Author abstract) 10 Refs.

关键词： elasticity monolayer linoleic acid

Potential regulation of linoleic acid and volatile organic compound contents in meat of chickens by PLCD1

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Journal of Integrative Agriculture 2023年第1期22卷 222-234页

作者： LIU Xiao-jing WANG Yong-li LIU Li LIU Lu ZHAO Gui-ping WEN Jie JIA Ya-xiong CUI Huan-xian State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Omega-3(linolenic acid(ALA),docosapentaenoic acid,eicosapentaenoic acid)and omega-6(linoleic acid(LA),arachidonic acid)polyunsaturated fatty acids are essential for health and normal physiological functioning in *** we report a genome-wide association study(GWAS)on LA content in chicken *** 19 significant single nucleotide polymorphisms(SNPs)identified by the GWAS approach were annotated in VILL,PLCD1 and OXSR1 genes with highly polymorphic linkage blocks,and explained 4.5%of the phenotypic variation in the LA ***,the PLCD1 mRNA expression level was negatively correlated with the LA content,and significantly higher in chickens with low LA content than in those with high LA *** addition,PLCD1 was found to be involved in metabolic pathways,***,the LA content was correlated with volatile organic compounds(e.g.,octanal,etc.),but no relationship was found with intramuscular fat and triglycerides in chicken *** results indicated that there are key SNPs in PLCD1 that regulate the content of LA,and it has no significant effect on fat deposition,but may affect the content of volatile organic compounds(VOCs).

关键词： linoleic acid flavor quality intramuscular fat PLCD1 chicken meat

Transcriptome-based analysis of key genes and pathways affecting the linoleic acid content in chickens

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Journal of Integrative Agriculture 2023年第12期22卷 3744-3754页

作者： ZHAO Wen-juan YUAN Xiao-ya XIANG Hai MA Zheng CUI Huan-xian LI Hua ZHAO Gui-ping Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding Foshan UniversityFoshan 528000P.R.China Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193P.R.China

linoleic acid is an essential polyunsaturated fatty acid that cannot be synthesized by humans or animals themselves and can only be obtained *** amount of linoleic acid present has an impact on the quality and flavour of meat and indirectly affects consumer ***,the molecular mechanisms influencing the deposition of linoleic acid in organisms are not *** the molecular mechanisms of linoleic acid deposition are not well understood,to investigate the main effector genes affecting the linoleic acid content,this study aimed to screen for hub genes in slow-type yellow-feathered chickens by transcriptome sequencing(RNA-Seq)and weighted gene coexpression network analysis(WGCNA).We screened for candidate genes associated with the linoleic acid content in slow-type yellow-feathered broilers.A total of 399 Tiannong partridge chickens were slaughtered at 126 days of age,fatty acid levels were measured in pectoral muscle,and pectoral muscle tissue was collected for transcriptome *** sequencing results were combined with phenotypes for WGCNA to screen for candidate *** enrichment analysis was also performed on the genes that were significantly enriched in the modules with the highest correlation.A total of 13310 genes were identified after quality control of transcriptomic data from 399 pectoral muscle *** was performed,and a total of 26 modules were obtained,eight of which were highly correlated with the linoleic acid *** key genes,namely,MDH2,ATP5B,RPL7A and PDGFRA,were screened according to the criteria|GS|>0.2 and|MM|>*** functional enrichment results showed that the genes within the target modules were mainly enriched in metabolic *** this study,a large-sample-size transcriptome analysis revealed that metabolic pathways play an important role in the regulation of the linoleic acid content in Tiannong partridge chickens,and MDH2,ATP5B,RPL7A and PDGFRA were screened as important candidate

关键词： chicken linoleic acid transcriptome sequencing weighted gene coexpression network analysis(WGCNA) metabolic pathways

Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第12期11卷 923-930页

作者： Xiao-feng LU Guo-qing HE Hai-ning YU Qi MA Sheng-rong SHEN Undurti N. DAS Department of Food Science and Nutrition College of Biosystems Engineering and Food Science Zhejiang University Hangzhou 310029 China College of Pharmaceutical Sciences Zhejiang University of Technology Hangzhou 310032 China UND Life Sciences Shaker Heights OH 44120 USA College of Medicine Bharati Vidyapeeth University Pune 411030 India

Polyunsaturated fatty acids （PUFAs） possess anti-cancer action both in vitro and in vivo. In the present study, we detected cell viability with methyl thiazolyl tetrazolium （MTT） assay and cell membrane permeability with propidium iodide （PI） fluorescence dyeing, and calculated cell membrane fluidity change as fluorescence anisotropy. Fatty acid content in cells was measured by gas chromatography/mass spectroscopy （GC/MS）, and the relationship between fatty acid composition and cell viability was studied. We observed that n-6 PUFA linoleic acid （LA） inhibited tumor cell growth at high concentrations （〉300 μmol/L）, while low concentrations （100-200 μmol/L） seemed to promote cell proliferation. Analyses of cell membrane permeability, cell membrane fluidity, and cell fatty acid composition suggested that the anti-cancer action of LA could be related to changes in the ratio of n-6 to n-3 PUFAs. We observed that pre-incubation of cancer cells with 100 μmol/L LA for 24 h enhanced cell sensitivity to the cytotoxic action of LA, whereas undifferentiated cell line LoVo seemed to have a distinct path in LA-induced death. These results showed that one of the mechanisms by which supplementation of LA induces cancer cell death could be altering the ratio of n-6/n-3 PUFAs, and this may be related to celt differentiation status.

关键词： Anti-cancer Fatty acids composition linoleic acid In vitro

Preparation of Conjugated linoleic acid and Identification of Its Isomers

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Chinese Journal of Chemical Engineering 2003年第2期11卷 130-135页

作者：郭诤张根旺孙彦 Department of Biochemical Engineering School of Chemical Engineering and technology Department of Food Science and Engineering Zhengzhou Institute of Technology

Conjugated linoleic acid (CLA) is a kind of fatty acid with physiological activities and potential application prospect. A synthesis method of conjugated linoleic acid and a purification technology were studied. CLA was prepared and purified by urea-complexation and conjugation using safflower oil as raw material. The purity of CLA and total recovery of the product was more than 95% and 48%, respectively. The main isomers produced in alkali-catalyzed conjugation were identified by gas chromatography (GC) linked to mass spectrometry (MS) and Fourier transform infrared spectroscopy (FTIR). The total amount of the two main isomers (9cis, 11trons-and 10trans, 12cis-CLA) determined by GC was more than 90% of the product.

关键词： linoleic acid conjugated linoleic acid urea-complexation alkali-catalyzed conjugation identification safflower oil

Qualitative and Quantitative Analysis of linoleic acid in Polygonati Rhizoma

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Digital Chinese Medicine 2020年第3期3卷 180-187页

作者： ZHOU Zhun YUAN Han-Wen PENG Cai-Yun JIAN Yu-Qing ZHOU Xu-Dong LI Bin SHENG Wen-Bing GONG Li-Min HE Shu-Jin WANG Wei LIU Chang-Xiao Sino-Pakistan TCM and Ethnomedicine Research Center Innovative Materia Medica Research InstituteSchool of PharmacyHunan University of Chinese MedicineChangshaHunan 410208China Hunan Xinhui Pharmaceutical Co. Ltd.ChangshaHunan 410000China Tianjin Institute of Pharmaceutical Research Tianjin 300193China

Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality *** The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid *** chromatogram was improved significantly after optimization for qualitative *** The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest *** study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).

关键词： Polygonati Rhizoma(Huang Jing,黄精) linoleic acid Qualitative analysis Quantitative analysis Thin layer chromatography(TLC) High performance liquid chromatography(HPLC)

A dose-response meta-analysis of α-linolenic acid and linoleic acid consumption and prostate cancer

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A dose-response meta-analysis of α-linolenic acid and linol...

Preparation of Conjugated linoleic acid of High Purity and Its Apparent Kinetic Characteristics During Formation

产业竞争力与创新驱动——2014年山东省科协学术年会

作者： Lomg Zhai Yili Wu Dongfeng Zhang Department of epidemiology and health statistics Qingdao University Medical college

Background:Epidemiological studies evaluating the association of a-linolenic acid（ALA） and linoleic acid（LA） with prostate cancer（PCa） risk have produced inconsistent ***:Pertinent studies were identified by a search of MEDLINE and *** or random effect pooled measure was selected on the basis of homogeneity test among ***-response relationship was assessed by generalized least-squares trend estimation analysis and restricted cubic ***:Overall,15 studies with 276748 participants for ALA and 11 studies including 222840 participants for LA were *** overall combined relative risk（95% confidence interval） of PCa for the highest *** category of ALA and LA intake was 1.043（0.863-1.262） and 0.921（0.819-1.034）,*** stratified analysis by study design,the association of dietary ALA intake with PCa was significant among cohort studies with relative risk being 0.886（0.805-0.974）.Within prospective studies linear doseresponse relationship was found,and the risk of PCa decreased by 5.8%（RR=0.942,95% CI=0.901-0.985,P=0.01） for every 1.0 g/d increment in ALA ***:Results from prospective studies provide evidence of a protective effect of dietary ALA consumption on *** intake is not significantly associated with PCa *** studies,particularly prospective studies,are needed.

关键词： α-linolenic acid linoleic acid Prostate cancer Dose-response meta-analysis

来源： cnki会议评论

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Transactions of Tianjin University 2002年第1期8卷 27-32页

作者：唐向阳郭诤张根旺天津大学理学院天津300072 天津大学化工学院天津300072 郑州工程学院食品工程系郑州450052

Conjugated linoleic acid (CLA) is a fatty acid with physiological activities and potential application prospect. This paper focuses on the method of synthesis of conjugated linoleic acid of high purity and the process line and conditions for its purification that can be used in large scale production. CLA of more than 95% purity was prepared by means of urea adduct purification and conjugation using safflower oil as material. The total recovery of the product adds up to more than 48%. The reactive kinetics about linoleic acid from sunflower oil converted into CLA was investigated, and its apparent kinetic model was also established, which can be used as a base for industrial designs.

关键词： linoleic acid conjugated linoleic acid preparation apparent kinetics urea adduct alkali catalyzed conjugation safflower oil

不同浓度维生素C对亚油酸微团的促过氧化和抗过氧化作用

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Acta Biochimica et Biophysica Sinica 1996年第1期28卷 101-105页

作者：于颖彦冈田茂日本冈山大学医学部病理学教室河北医学院附属第二医院病理科

不同浓度维生素Ｃ对亚油酸微团的促过氧化和抗过氧化作用于颖彦（河北医学院附属第二医院病理科石家庄０５００００）冈田茂（日本冈山大学医学部病理学教室７００）关键词亚油酸，脂质过氧化，铁离子，维生素Ｃ维生素Ｏ（简称Ｖｃ）在... 详细信息

不同浓度维生素Ｃ对亚油酸微团的促过氧化和抗过氧化作用于颖彦（河北医学院附属第二医院病理科石家庄０５００００）冈田茂（日本冈山大学医学部病理学教室７００）关键词亚油酸，脂质过氧化，铁离子，维生素Ｃ维生素Ｏ（简称Ｖｃ）在人体内除了参与激素、神经传导介质和...

关键词： linoleic acid Lipid peroxidation Iron ion Vitamin C