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Effects of uniconazole with or without micronutrient on the lignin biosynthesis,lodging resistance,and winter wheat production in semiarid regions

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Journal of Integrative Agriculture 2020年第1期19卷 62-77页

作者： Irshad AHMAD MENG Xiang-ping Muhammad KAMRAN Shahzad ALI Shakeel AHMAD LIU Tie-ning CAI Tie HAN Qing-fang Key Laboratory of Crop Physio-Ecology and Tillage Science in North-Western Loess Plateau Ministry of Agriculture/College of AgronomyNorthwest A&F UniversityYangling 712100P.R.China Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas Ministry of Education/Institute of Water Saving Agriculture in Arid Areas of ChinaNorthwest A&F UniversityYangling 712100P.R.China

Lodging stress results in grain yield and quality reduction in wheat. Uniconazole, a potential plant growth regulator significantly enhances lignin biosynthesis and thus provides mechanical strength to plants in order to cope with lodging stress. A field study was conducted during the 2015–2016 and 2016–2017 growing seasons, to investigate the effects of uniconazole sole application or with micronutrient on the lignin biosynthesis, lodging resistance, and production of winter wheat. In the first experiment, uniconazole at concentrations of 0(CK), 15(US1), 30(US2), and 45(US3) mg L^-1 was applied as sole seed soaking, while in the second experiment with manganese(Mn) at concentration of 0.06 g L^-1 Mn, 0.06 g L^-1 Mn+ 15 mg L^-1 uniconazole(UMS1), 0.06 g L^-1 Mn+30 mg L^-1 uniconazole(UMS2), and 0.06 g L^-1 Mn+45 mg L^-1 uniconazole(UMS3), respectively. Uniconazole sole application or with micronutrient significantly increased the lignin content by improving the lignin-related enzyme activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, tyrosine ammonialyase, and peroxidase, ameliorating basal internode characteristics, and breaking strength. The spike length, spike diameter, spikes/plant, weight/spike, yield/spike, and grain yield increased and then decreased with uniconazole application at a higher concentration, where their maximum values were recorded with UMS2 and US2 treatments. The lignin accumulation was positively correlated with lignin-related enzyme activities and breaking strength while, negatively correlated with lodging rate. Uniconazole significantly improved the lignin biosynthesis, lodging resistance, and grain yield of winter wheat and the treatments which showed the greatest effects were uniconazole seed soaking with micronutrient at a concentration of 30 mg L^-1 and 0.06 g L^-1 , and uniconazole sole seed soaking at a concentration of 30 mg L^-1 .

关键词： lignin biosynthesis lodging resistance semi-arid regions manganese uniconazole wheat

Upgraded durian genome reveals the role of chromosome reshuffling during ancestral karyotype evolution,lignin biosynthesis regulation,and stress tolerance

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Science China(Life Sciences) 2024年第6期67卷 1266-1279页

作者： Wanwan Li Xin Chen Jianing Yu Yuxian Zhu College of Life Sciences Shaanxi Normal UniversityXi’an 710119China The State Key Laboratory of Protein and Gene Research College of Life SciencesPeking UniversityBeijing 100871China The Institute for Advanced Studies Wuhan UniversityWuhan 430072China

Durian(Durio zibethinus)is a tropical fruit that has a unique flavor and *** occupies a significant phylogenetic position within the Malvaceae *** core-eudicot plants are reported to share seven ancestral karyotypes that have undergone reshuffling,resulting in an abundant genomic ***,the ancestral karyotypes of the Malvaceae family,as well as the evolution trajectory leading to the28 chromosomes in durian,remain poorly ***,we report the high-quality assembly of the durian genome with comprehensive comparative genomic *** analyzing the collinear blocks between cacao and durian,we inferred 11 Malvaceae ancestral *** blocks were present in a single-copy form in cacao and mainly in triplicates in durian,possibly resulting from a recent whole genome triplication(WGT)event that led to hexaploidization of the durian genome around 20(17–24)million years ago.A large proportion of the duplicated genes in durian,such as those involved in the lignin biosynthesis module for phenylpropane biosynthesis,are derived directly from whole genome duplication,which makes it an important force in reshaping its genomic *** studies have revealed that genes involved in feruloyl-Co A formations were highly preferentially expressed in fruit peels,indicating that the thorns produced on durian fruit may comprise guaiacyl and syringyl *** all the analyzed transcription factors(TFs),members of the heat shock factor family(HSF)were the most significantly upregulated under heat *** subfamilies of genes encoding heat shock proteins(HSPs)in the durian genome appear to have undergone *** potential interactions between HSF Dzi05.397 and HSPs were examined and experimentally *** study provides a high-quality durian genome and reveals the reshuffling mechanism of ancestral Malvaceae chromosomes to produce the durian *** also provide insights into the mechanism underlying lignin bi

关键词： Durio zibethinus whole genome duplication whole genome triplication Malvaceae ancestral karyotypes chromosome reshuffling lignin biosynthesis heat stress

PagERF81 regulates lignin biosynthesis and xylem cell differentiation in poplar

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Journal of Integrative Plant Biology 2023年第5期65卷 1134-1146页

作者： Xin-Wei Zhao Qiao Wang Dian Wang Wei Guo Meng-Xuan Hu Ying-Li Liu Gong-Ke Zhou Guo-Hua Chai Shu-Tang Zhao Meng-Zhu Lu State Key Laboratory of Tree Genetics and Breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091China Collegeof Resources and Environment QingdaoAgricultural UniversityQingdao 266109China CollegeofAgronomy QingdaoAgriculturalUniversityQingdao 266109China TaishanAcademyof Forestry Sciences Taian271000China College of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdao 266109China State Key Laboratory of Subtropical Silviculture College of Forestry and BiotechnologyZhejiang A&F UniversityHangzhou 311300China

lignin is a major component of plant cell walls and is essential for plant growth and development. lignin biosynthesis is controlled by a hierarchical regulatory network involving multiple transcription factors. In this study, we showed that the gene encoding an APETALA 2/ethylene-responsive element binding factor(AP2/ERF) transcription factor, PagERF81,from poplar 84 K(Populus alba × P. glandulosa) is highly expressed in expanding secondary xylem cells. Two independent homozygous Pagerf81 mutant lines created by gene editing, produced significantly more but smaller vessel cells and longer fiber cells with more lignin in cell walls, while PagERF81 overexpression lines had less lignin,compared to non-transgenic controls. Transcriptome and reverse transcription quantitative PCR data revealed that multiple lignin biosynthesis genes including Cinnamoyl CoA reductase 1(PagCCR1),Cinnamyl alcohol dehydrogenase 6(PagCAD6), and 4-Coumarate-CoA ligase-like 9(Pag4CLL9) were upregulated in Pagerf81 mutants, but down-regulated in PagERF81 overexpression lines. In addition, a transient transactivation assay revealed that PagERF81 repressed the transcription of these three ***, yeast one hybrid and electrophoretic mobility shift assays showed that PagERF81 directly bound to a GCC sequence in the PagCCR1 promoter. No known vessel or fiber cell differentiation related genes were differentially expressed, so the smaller vessel cells and longer fiber cells observed in the Pagerf81 lines might be caused by abnormal lignin deposition in the secondary cell walls. This study provides insight into the regulation of lignin biosynthesis, and a molecular tool to engineer wood with high lignin content, which would contribute to the lignin-related chemical industry and carbon sequestration.

关键词： lignin biosynthesis PagERF81 Populus secondary cell wall xylem development

Transcriptome and Metabolome Analysis Revealed that lignin biosynthesis Contributed to Build the Defence Responses of Bayberry(Myrica rubra) Against Twig Blight

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Transcriptome and Metabolome Analysis Revealed that Lignin B...

Effect of the suppression of BpAP1 on the expression of lignin related genes in birch

中国植物病理学会2024年学术年会

作者： GUO Junning LI Gang YU Zheping LI Bin REN Haiying Institute of Biotechnology Zhejiang University Institute of Horticulture Institute of Agro-product Safety and NutritionZhejiang Academy of Agricultural Sciences

Twig blight,a highly destructive disease primarily caused by Pestalotiopsis versicolor,harms bayberry plantations and ***,the molecular and regulatory defence responses to twig blight remains ***,we inoculated a susceptible cultivar bayberry variety with *** XJ27 and documented the bayberry *** inoculation,the bayberry disease index rose ***,we conducted integrative transcriptome and metabolome analyses to elucidate the key genes and secondary metabolites changes in susceptible bayberry *** results revealed a notable increase in the expression of genes and metabolites related to phenylpropanoid metabolism,particularly lignin biosynthesis,after pathogen infection.A total of 9588 DEGs were identified in the present study,and seven of those had candidate structural genes(PAL,4 CL,HCT,COMT,CAD,CCR,and CCoAOMT) that were specifically related to lignin biosynthesis in bayberry ***,seven upregulated DAMS,L-phenylalanine,coniferin,p-coumaric acid,cinnamaldehyde,eleutheroside B,ferulaldehyde,and isoeugenol,were enriched in the phenylpropanoid biosynthesis *** observations suggested a potential role for lignin biosynthesis in bayberry defence responses to twig *** further support this hypothesis,quantitative real-time PCR analysis confirmed higher expression levels of pivotal lignin synthesis genes in infected *** also reinforced the conclusion that lignin metabolism enhanced bayberry defence by reporting an increase in total lignin content in infected *** summary,our findings provide valuable insights into the defence responses to bayberry twig blight.

关键词： Myrica rubra Twig blight Transcriptome Metabolome Defence responses lignin biosynthesis

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Journal of Forestry Research 2022年第1期33卷 289-297页

作者： Haijiao Huang Shuo Wang Huiyu Li Jing Jiang State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China

lignin is an integral part of secondary cell walls in plants and plays important roles in maintaining the strength of stems,enhancing transport ability of stems,and providing resistance to multiple *** biosynthesis has become one of the hotspots in molecular forest biology *** AP1 transcription factor plays important roles in plant fower ***,in this study,suppression of BpAP1 altered the transcription profiles of white birch and RNA-seq was used to find that suppression of BpAP1 changed the expression of lignin pathway-related genes;C4 H/CYP73A,POD were down-regulated and HCT,CCoAOMT,REF1 and CAD were *** walls of the suppressed transgenic birch were significantly thinner than the wild type of birch,and Bp AP1-repressed birch contained less *** addition to regulation of foral development,BpAP1 might play a role in regulating the expression of genes in lignin biosynthesis of *** study could provide a new insight into the function of AP1 genes in woody species.

关键词： BpAP1 lignin biosynthesis RNA-seq Wood fiber cell walls

Simultaneously disrupting AtPrx2,AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem

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Journal of Integrative Plant Biology 2015年第4期57卷 349-356页

作者： Jun Shigeto Yoshitaka Itoh Sakie Hirao Kaori Ohira Koki Fujita Yuji Tsutsumi Faculty of Agriculture Kyushu University

Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-D NA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl （β-O-4） linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants oexhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved ceil wall degradability. This study will contribute to progress in lignin bioengineering to improve iignocellulosic biomass.

关键词： Arabidopsis knockout mutant lignin biosynthesis plantperoxidase

Investigation of genome structure of a cinnamyl alcohol dehydrogenase locus in a basal angiosperm hardwood species, Liriodendron tulipifera L., reveals low synteny

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Journal of Systematics and Evolution 2011年第5期49卷 396-405页

作者： Scott E.SCHLARBAUM Department of Forestry Wildlife and FisheriesInstitute of AgricultureThe University of Tennessee

Basal angiosperms contain a wide diversity of floral and growth forms and gave rise to the largest recent angiosperm *** none of the basal angiosperm genomes has been sequenced,examining large bacterial artificial chromosome(BAC) inserts remains the main approach to providing a first glimpse of the structure and organization of their *** this study,we sequenced a 126.9-kbp BAC contig harboring a cinnamyl alcohol dehydrogenase gene(LtuCAD1) in a basal angiosperm species,Liriodendron tulipifera L.,an important timber tree species with significant ecological and economic values.A key enzyme in lignin biosynthesis,CAD catalyzes the final step in the synthesis of *** carried out phylogenetic analyses of seven full-length CAD family genes(LtuCAD1-7) obtained from a comprehensive Liriodendron expressed sequence tag *** phylogenetic tree suggests that LtuCAD1 is the primary CAD gene involved in lignifications as it is the only Liriodendron CAD grouped with the bona fide CADs *** well as the LtuCAD1,the BAC contig contained fragmented sequences for one integrase,eight hypothetical proteins,two gag-pol polyproteins,one RNase H family protein,and one chromatin binding *** analysis with other angiosperm species suggests that the genomic segment in this BAC has undergone frequent *** study is our initial step in identifying and understanding lignin biosynthesis genes from basal angiosperm *** knowledge can help bridge the information gap between hardwood(angiosperm) and softwood(gymnosperm) species and benefit potential breeding and biotechnology application for enhanced production of biomass and digestibility in ***.

关键词： BAC contig cinnamyl alcohol dehydrogenase(CAD) gene order lignin biosynthesis synteny.

Overexpression of the peroxidase gene ZmPRX1 increases maize seedling drought tolerance by promoting root development and lignification

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The Crop Journal 2024年第3期12卷 753-765页

作者： Xiuzhen Zhai Xiaocui Yan Tinashe Zenda Nan Wang Anyi Dong Qian Yang Yuan Zhong Yue Xing Huijun Duan State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China Ministry of EducationCollege of AgronomyHebei Agricultural UniversityBaoding 071001HebeiChina

Drought is a main abiotic stress factor hindering plant growth,development,and crop ***,it is crucial to understand the mechanisms by which plants cope with drought ***,the function of the maize peroxidase gene ZmPRX1 in drought stress tolerance was investigated by measurement of its expression in response to drought treatment both in a ZmPRX1 overexpression line and a mutant *** higher root lignin accumulation and seedling survival rate of the overexpression line than that of the wild type or mutant support a role for ZmPRX1 in maize drought tolerance by regulating root development and ***,yeast one-hybrid,Dule luciferase and ChIP-qPCR assays showed that ZmPRX1 is negatively regulated by a nuclear-localized ZmWRKY86 transcription *** gene could potentially be used for breeding of drought-tolerant cultivars.

关键词： Drought tolerance Maize ZmPRX1 Root development lignin biosynthesis

The application of Fourier transform mid-infrared(FTIR) spectroscopy to identify variation in cell wall composition of Setaria italica ecotypes

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Journal of Integrative Agriculture 2017年第6期16卷 1256-1267页

作者： Christopher Brown Antony P.Martin Christopher P.L.Grof Centre for Plant Science School of Environmental and Life Sciences University of Newcastle Newcastle 2308 Australia Light Sheet Microscopy Unit Hunter Medical Research Institute Newcastle 2305 Australia

Cell wall composition in monocotyledonous grasses has been identified as a key area of research for developing better feedstocks for forage and biofuel *** viridis and its close domesticated relative Setaria italica have been chosen as suitable monocotyledonous models for plants possessing the C4 pathway of photosynthesis including sorghum,maize,sugarcane,switchgrass and Miscanthus×*** partial least squares regression（PLSR）models to predict *** stem composition have been generated,based upon Fourier transform mid-infrared（FTIR）spectra and calibrated with wet chemistry determinations of ground *** stem material measured using a modified version of the US National Renewable Energy Laboratory（NREL）acid hydrolysis *** models facilitated a high-throughput screening analysis for glucan,xylan,Klason lignin and acid soluble lignin（ASL）in a collection of 183 natural *** variants and clustered them into classes,some possessing unique *** predictive models provide a highly efficient screening tool for large scale breeding programs aimed at identifying lines or mutants possessing unique cell wall *** encoding key catalytic enzymes of the lignin biosynthesis pathway exhibit a high level of conservation with matching expression profiles,measured by RT-q PCR,among accessions of ***,which closely mirror profiles observed in the different developmental regions of an elongating internode of *** by RNASeq.

关键词： monocotyledonous C4 grasses lignin biosynthesis Fourier transform mid-infrared spectroscopy Setaria italica