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Neural Regeneration Research 2024年第2期19卷 277-281页

作者： Óscar Darío García-García Víctor Carriel Jesús Chato-Astrain Department of Histology Tissue Engineering GroupUniversity of Granada&Instituto de Investigación Biosanitaria ibs.GRANADAGranadaSpain

The myelin sheath is a lipoprotein-rich,multilayered structure capable of increasing conduction velocity in central and peripheral myelinated nerve *** to the complex structure and composition of myelin,various histological techniques have been developed over the centuries to evaluate myelin under normal,pathological or experimental ***,methods to assess myelin integrity or content are key tools in both clinical diagnosis and neuroscience *** this review,we provide an updated summary of the composition and structure of the myelin sheath and discuss some histological procedures,from tissue fixation and processing techniques to the most used and practical myelin histological staining *** the lipoprotein nature of myelin,the main features and technical details of the different available methods that can be used to evaluate the lipid or protein components of myelin are described,as well as the precise ultrastructural techniques.

关键词： fluorescence microscopy histology light microscopy lipid histochemistry metallographic techniques myelin histochemistry myelin immunohistochemistry myelin structure&composition myelin ultrastructural evaluation tissue fixation&processing

Parasitism of Pieris rapae （Lepidoptera： Pieridae） by the endoparasitic wasp Pteromalus puparum （Hymenoptera： Pteromalidae）： Effects of parasitism on differential hemocyte counts, micro- and ultra-structures of host hemocytes

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Insect Science 2012年第4期19卷 485-497页

作者： Qian-Qian Zhang Jia Huang Jia-Ying Zhu Gong-Yin Ye State Key Laboratory of Rice Biology&Key laboratory of Molecular Biology of Crop Pathology and Insects of Ministry of Agriculture Institute of Insect SciencesZhejiang UniversityHangzhouChina

Parasitism by the endoparasitic wasp Pteromalus puparum （Hymenoptera： Pteromalidae） by using only its associated venom, can suppress the immunal responses of Pieris rapae （Lepidoptera： Pieridae）. However, up to now, current knowledge of the mech- anisms has been limited. The response of host hemocytes to parasitism was investigated using a combination of light and transmission electron microscopy （TEM）. Five hemocyte types, prohemocytes （PRs）, granulocytes （GRs）, plasmatocytes （PLs）, oenocytoids （OEs） and coagulocytes （COs）, were observed and characterized from both unparasitized and parasitized Pieris rapae pupae. light microscopy showed that both GRs and PLs became more round and spread abnormally after parasitism, whereas the shape of other types of hemocytes remained unaffected. In addition, the size of PRs and PLs became larger while OEs became smaller. The proportion of PRs significantly increased after parasitism and that of PLs decreased by 43.9%, but there was no significant increase of GRs and OEs. TEM showed that all types of hemocytes except COs were damaged to various degrees after parasitism, especially resulting in electron opaque cytoplasm and nucleus, fewer cell organelles of rough endoplasmic reticulum, mitochondria and vesicles. Our results indicate that parasitism by P. puparum affects differential hemocyte counts and structures of host hemocytes, particularly for GRs and PLs, which may be the main cause of the parasitoid suppressing host cellular immune responses.

关键词： Key words hemocytes light microscopy parasitism Pieris rapae Pteromaluspuparum,transmission electron microscopy ultrastructure

Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

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World Journal of Gastroenterology 2010年第5期16卷 563-570页

作者： Flavio M Greggio Ricardo BV Fontes Laura B Maifrino Patricia Castelucci Romeu Rodrigues de Souza Edson A Liberti Autonomic Nervous System Laboratory (VQM) Department of Anatomy Instituto de Ciencias Biomedicas da Universidade de Sao Paulo 2415 Av Prof Lineu Prestes 05508-900 So Paulo Brazil

AIM:To evaluate effects of preand postnatal protein deprivation and postnatal recovery on the myenteric plexus of the rat esophagus. METHODS: Three groups of young Wistar rats (aged 42 d) were studied: normalfed (N42), proteindeprived (D42), and proteinrecovered (R42). The myenteric neurons of their esophagi were evaluated by histochemical reactions for nicotinamide adenine dinucleotide (NADH), nitrergic neurons (NADPH)diaphorase and acetylcholinesterase (AChE), immunohistochemical reaction for vasoactive intestinal polypeptide (VIP), and ultrastructural analysis by transmission electron ***: The cytoplasms of large and medium neurons from the N42 and R42 groups were intensely reactive for NADH. Only a few large neurons from the D42 group exhibited this aspect. NADPH detected in the D42 group exhibited low reactivity. The AChE reactivity was diffuse in neurons from the D42 and R42 groups. The density of large and small varicosities detected by immunohistochemical staining of VIP was low in ganglia from the D42 group. In many neurons from the D42 group, the double membrane of the nuclear envelope and the perinuclear cisterna were not detectable. NADH and NADPH histochemistry revealed no group differences in the prof ile of nerve cell perikarya (ranging from 200 to 400 μm2).CONCLUSION: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons.

关键词： Esophagus Enteric nervous system Myenteric plexus Proteins light microscopy Transmission electron microscopy

Protective effect of basic fibroblast growth factor on laser induced retinopathy

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International Journal of Ophthalmology(English edition) 2013年第6期6卷 744-751页

作者： Unal Kartal Emel Koptagel H.Eray Bulut Haydar Erdogan Department of Histology and Embryology Faculty of MedicineCumhuriyet University Department of Ophtalmology Faculty of MedicineCumhuriyet University

AIM:To investigate the side effects of the commonly used laser treatment along with testing the neuroprotective effect of bFGF on a potential retinal ***:To do this,30 chinchilla pigmented adult male rabbits were divided into the control and experimental *** control and experimental groups underwent both laser application and bFGF *** retinal tissue impairment and its renewal rate were tested under the light and electron microscopical ***:The focal laser application on rabbit eyes caused morphological alterations both in the application region and in the neighbouring *** the damaged areas,the outer nuclear layer of the neural retina was almost disappeared,retina pigment epithelium was interrupted,the retina pigment epithelium migrated intraretinally,and the damaged region along with neighbouring areas seemed to be not *** application just after the laser photocoagulation,revealed better results in application ***:It could be suggested that the bFGF application following laser photocoagulation might have protective,repairing and wound healing effects on the retina.

关键词： retina bFGF laser photocoagulation rabbit light microscopy electron microscopy

AN ENZYMOCYTOCHEMICAL STUDY OF ALKALINE PHOSPHATASE ISOENZYMES IN GASTRIC CANCER-light AND ELECTRON MICROSCOPIC OBSERVATIONS

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Chinese Journal of Cancer Research 1995年第2期7卷 141-147页

作者：苏英豪杨光霖董聿明张红吴继锋王道斌 Department of Pathology Anhui Medical University Hefei

By using light and electron microscopic cytochemical technique, the activities and distributions of AP isoenzymes in gastric cancers and benign gastric diseases were *** results showed: Nagao, Regan and Kasahara isoenzymes, which were not expressed in normal gastric mucosae and non-malignant lesions, might be considered as tumor markers of gastric cancer;The epithelium of intestinal metaplasia exhibited intestinal-type AP only,which was one of the markers of well-differentiated intestinal *** the view-point of the gene expression of AP isoenzymes, two mutation hypothesis and recessive-gene mutation hypothesis might be fit for gastric cancer.

关键词： Alkaline phospbatase isoenzymes Gastric cancer Cytochemistry light microscopy Electron microscopy.

A two-step superplastic forging forming of semi-continuously cast AZ70 magnesium alloy

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Journal of Magnesium and Alloys 2015年第1期3卷 70-75页

作者： Pan Wang Shijie Zhu Liguo Wang Lihong Wu Shaokang Guan School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450002China

A two-step technology combined forging with superplastic forming has been developed to enhance the forgeability of semi-continuously cast AZ70 magnesium alloy and realize the application of the as-cast magnesium alloy in large deformation bullet *** the first step,fine-grained microstructure preforms that are suitable for superplastic forming were obtained by reasonably designing the size of the initial blanks with the specific height-to-diameter ratio,upsetting the blanks and subsequent *** the second step,the heat treated preforms were forged into the end products at the superplastic *** end products exhibit high quality surface and satisfied ***,this forming technology that not only avoids complicating the material preparation but also utilizes higher strain rate superplastic provides a near net-shaped novel method on magnesium forging forming technology using as-cast billet.

关键词： Magnesium alloy Superplasticity Recrystallization Casting light microscopy Forging technology

Possible Relevance of the Allende Meteorite Conditions in Prebiotic Chemistry: An Insight into the Chondrules and Organic Compounds

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Advances in Biological Chemistry 2023年第3期13卷 82-99页

作者： Alejandro Heredia Barbero Héctor G. Vázquez López Adriana L. Meléndez López Jorge A. Cruz Castañeda Daniel Luna Laviada Karina E. Cervantes de la Cruz Victor Meza Laguna Vladimir A. Basiuk Ivonne Rosales Chávez Alicia Negrón Mendoza Sergio Ramos Bernal Laboratorio de Evolución Química Departamento de Química de Radiaciones y Radioquímica Instituto de Ciencias Nucleares Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México Programa de Posgrado en Ciencias Químicas Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México Departamento de Física Facultad de Ciencias Carrera de Ciencias de la Tierra Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México Departamento de Física de Plasmas e Interacción de Radiación con Materia Instituto de Ciencias Nucleares Ciudad Universitaria Ciudad de México México Departamento de Física y Química Teórica Facultad de Química Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México

The study of the mineral and organic content of the Allende meteorite is important for our understanding of the molecular evolution of the universe as well as the ancient Earth. Previous studies have characterized the magnetic minerals present in ordinary and carbonaceous chondrites, providing information on the evolution of magnetic fields. The interaction of organic compounds with magnetic minerals is a possible source of chemical diversity, which is crucial for molecular evolution. Carbon compounds in meteorites are of great scientific interest for a variety of reasons, such as their relevance to the origins of chirality in living organisms. This study presents the characterization of organic and mineral compounds in the Allende meteorite. The structural and physicochemical characterization of the Allende meteorite was accomplished through light microscopy, powder X-ray diffraction with complementary Rietveld refinement, Raman and infrared spectroscopy, mass spectrometry, scanning electron microscopy, and atomic force microscopy using magnetic signal methods to determine the complex structure and the interaction of organic compounds with magnetic Ni-Fe minerals. The presence of Liesegang-like patterns of chondrules in fragments of the Allende structure may also be relevant to understanding how the meteorite was formed. Other observations include the presence of magnetic materials and nanorod-like solids with relatively similar sizes as well as the heterogeneous distribution of carbon in chondrules. Signals observed in the Raman and infrared spectra resemble organic compounds such as carbon nanotubes and peptide-like molecules that have been previously reported in other meteorites, making the Mexican Allende meteorite a feasible sample for the study of the early Earth and exoplanetary bodies.

关键词： Allende Meteorite Carbonaceous Chondrite light microscopy X-Ray Diffraction with the Rietveld Method Raman Spectroscopy Attenuated Total Reflectance Infrared Spectroscopy Mass Spectrometry Scanning Electron Mi-croscopy Energy Dispersive X-Ray Spectroscopy Magnetic Force microscopy

Non-Diabetic Kidney Disease in Type 2 Diabetes Mellitus: A Study of 82 Patients and Review of the Literatures

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Open Journal of Nephrology 2022年第1期12卷 169-186页

作者： Ehab Mohammed Issa Al Salmi Dawood Al Riyami Saif Khan Marwa Al Riyami Fatma Al Rahbi Suad Hannawi The Renal Medicine Department The Royal Hospital Muscat Oman The Medicine Department Oman Medical Specialty Board Muscat Oman The Renal Medicine Department SQUH Muscat Oman The Pathology Department SQUH Muscat Oman The Medicine Department MOHAP Dubai UAE

Background: Worldwide, diabetic nephropathy-DN is the leading cause of end-stage kidney disease-ESKD, DN is a common cause of renal failure with a reported frequency of 10% - 15% in type-2-diabetes-mellitus-T2DM patients, however there is a great discrepancy between countries. The aim of the pre-sent study is to evaluate the findings of kidney biopsies performed on diabetic patients. Materials and Methods: We studied native kidney histopathological findings in the period from January 2016 till end of December 2018 done for patients with T2DM with chronic kidney diseases-CKD. Results: A total of 82 DM-patients, 50 males (61%) and 32 females (39%) with age mean (95% CI) of 50.8 (47.1 - 55.2) years for all patients, ranged between 15 to 65 years. Histological findings showed that 57.3% of patients had DN. While focal-segmental-glomerulosclerosis-FSGS was present in 20.7%—primary in 8.6% and secondary in 12.1%. IgA represented 4.9%, while Lupus nephritis, Membranous and drug induced interstitial nephritis were each present in 3.7%. MCD was present in 2.4%. Lastly diffuse proliferative GN, ANCA associated glomerulonephritis, and hypertensive nephrosclerosis accounted for 1.2%. Conclusion: The prevalence of NDKD is remarkably frequent in DM patients who underwent kidney biopsy and FSGS was the most frequent diagnosis. To get a proper histopathological diagnosis, an adequate tissue biopsy is needed with an adequate number of glomeruli. There is a great need for more consideration to biopsy diabetic patients, as the finding of NDKD requires a different therapeutic approach. This, hopefully, will help to manage these patients better and therefore, ameliorate the progression to ESKD over time and therefore delay the need for RRT.

关键词： Diabetic Nephropathy Diabetic Kidney Disease Histopathology light microscopy Electron microscopy Immunofluorescence Non-Diabetic Kidney Diseases Renal Replacement Therapy (RRT)

Stem Histopathology of Sesame Seedlings Infected with Alternaria alternata

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microscopy Research 2016年第2期4卷 11-19页

作者： Maryam Ajmal Abida Akram Anum Ara Shaista Akhund Brian Gagosh Nayyar Wajiha Seerat Department of Botany PMAS Arid Agriculture University Rawalpindi Pakistan

In the present study, histopathology of three varieties of sesame TS 3, TS 5 and SG 27 infected with Alternaria alternata was carried out to understand the mechanism of fungal infection and penetration in sesame plant as well as to determine the histological manifestation in sesame cells by light microscopy. Fungus was identified in infected tissues as a dark bluish black with toluidine blue O staining. light microscopic examination of sesame stem showed that the fungus was present in epidermis, hypodermis and cortical parenchyma tissue as the symptoms became visible by naked eye ten days after inoculation (DAI). As the disease progress, the fungus moved from cortical parenchyma to vascular bundle, xylem and phloem. Later on, it completely overlapped the vascular bundle and entered in pith. When necrotic lesion appeared, fungus was present abundantly in epidermis, hypodermis, cortical parenchyma, vascular bundles and in pith. Due to its excessive growth and complete overlapping of cells, disorganization or destruction of cells of sesame took place. It was concluded that the Alternaria alternata was not a tissue limited pathogen instead of this it spread in to all tissues of stem from epidermis to pith.

关键词： Sesame Alternaria alternata Pathogen Fungus light microscopy