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Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding,leukogram,protein profile and ileum histopathology

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Asian Pacific Journal of Tropical Medicine 2017年第3期10卷 240-248页

作者： Hamdy Soufy Nadia M.El-Beih Soad M.Nasr Tamer H.Abd El-Aziz Fathia A.M.Khalil Youssef F.Ahmed Hala A.A.Abou Zeina Department of Parasitology and Animal Diseases National Research Centre Department of Zoology Faculty of ScienceAin Shams University Department of Reproduction and Artificial Insemination National Research Centre

Objective:To investigate the activity of Egyptian propolis extracts(ethanol and water)on cryptosporidiosis in experimentally infected dexamethasone-immunosuppressed ***:A total of 180 male rats(190-220)g BWt were randomly divided into 9 equal groups(G1-G9).Groups of rats were kept as(G1):normal control,(G2-G9):immunosuppressed with dexamethasone and(G3-G9):infected with Cryptosporidium *** from(G4to G9)were given orally ethanol and water extract of propolis(at a dose of 50 mg/kg BWt)and nitazoxanide(standard anti-cryptosporidial drug at a dose of 100 mg/kg BWt)to infected rats with different *** pellets were collected from all groups to monitor oocysts shedding from the 2nd to the 15th day post *** the end of the experiment,blood was collected from all groups for determination of leukogram and serum *** specimens were also examined ***:The highest reduction of oocysts shedding in faecal samples was 88%in rats prophylactically treated with propolis ethanol extract at the 4th dpi,and in rats prophylactically treated with water extract of propolis,was91%at the 6th *** was a marked increase in neutrophils count and α_2-andβ-globulins levels in infected rats treated with both extracts,while a significant decrease was detected in lymphocytes compared to the infected non treated group.β-globulin level markedly increased in the rats administered *** changes were observed in the ileum of rats infected with ***:Egyptian propolis extracts have an activity on cryptosporidiosis in ***,propolis modulated the immunity in dexamethasone-immunosuppressed rats.

关键词： Cryptosporidiosis Propolis Rat model leukogram Serum proteins Histopathology

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Hematological parameters of a Neotropical wild frog population,with a phylogenetic perspective on blood cell composition in Anura

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Current Zoology 2022年第3期68卷 361-369页

作者： Lilian FRANCO-BELUSSI Diogo B.PROVETE Taynara R.F.LEÃO Mayara S.SIQUEIRA Bruno S.L.VALVERDE Brenda O.MARTINS Classius DE OLIVEIRA Carlos Eurico FERNANDES Instituto de Biociências Universidade Federal de Mato Grosso do SulCampo GrandeMato Grosso do Sul 79002970Brazil Gothenburg Global Biodiversity Centre GöteborgBox 100S 40530Sweden Graduate Program in Biodiversity.Instituto de Biociências Letras e Ciências ExatasUniversidade Estadual PaulistaSão Josédo Rio PretoSão PauloBrazil Graduate Program in Animal Biology.Instituto de Biociências Universidade Federal de Mato Grosso do SulCampo GrandeMato Grosso do SulBrazil Departamento de Biologia.Instituto de Biociências Letras e Ciências ExatasSão Josédo Rio PretoUniversidade Estadual PaulistaBrazil

Hematological parameters can provide key information to an animal health ***,this information is usually hard to ***,we described the hematological parameters of Leptodactylus podicipinus in the Brazilian *** measured red blood cell morphometrics,erythrogram,and *** also tested for phylogenetic signal in the erythrogram and leukogram of 48 frog species from 15 families,testing if body size explains their *** were the most abundant leukocytes(>60%)in ***,followed by neutrophils(∼10%).Given that *** is an abundant and widely distributed species in central Brazil,knowing its hematological pattern can help establish a baseline and improve its use as a bioindicator of environmental *** corpuscular hemoglobin and value contributed more to the phylomorphospace of erythrogram,in which Leptodactylus *** Hypsiboas raniceps had lower values of these variables,whereas Bufotes viridis and Hyla arborea had high *** phylogenetic signal was spread throughout the dimensions of the leukogram *** variables that most contributed to it were total leukocytes counts,lymphocytes,and *** also found a moderate phylogenetic signal for both the erythrogram and ***,body size accounted for a low proportion of variation in both the leukogram(4.7%)and erythrogram(0.57%).By applying phylogenetic comparative methods to hematological parameters,our results add a new perspective on the evolution of blood cell physiology in frogs.

关键词： erythrogram leukogram conservation physiology macroevolution population monitoring programs

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