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N-myc downstream regulated gene 1 inhibition of tumor progression in Caco2 cells

World Journal of Gastrointestinal Oncology 2022年第12期14卷 2313-2328页

作者： Yi-Xiao He Hong Shen Yu-Zhu Ji Hai-Rong Hua Yu Zhu Xiang-Fei Zeng Fang Wang Kai-Xin Wang Department of Pathology Mianyang Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaMianyang 621000Sichuan ProvinceChina Department of Pathology and Pathophysiology Faculty of Basic Medical SciencesKunming Medical UniversityKunming 650500Yunnan ProvinceChina Department of Pathology Zhaotong First People’s HospitalZhaotong 657000Yunnan ProvinceChina School of Nursing Henan Vocational College of Applied TechnologyKaifeng 450000Henan ProvinceChina Department of Clinical Pathology West China HospitalSichuan UniversityChengdu 610000Sichuan ProvinceChina Department of Pathology Huazhong University of Science and Technology Union Shenzhen Hospital(Nanshan Hospital)Shenzhen 518052Guangdong ProvinceChina

BACKGROUND Invasion and migration are the irreversible stages of colorectal cancer(CRC).The key is to find a sensitive,reliable molecular marker that can predict the migration of CRC at an early stage.N-myc downstream regulated gene 1(NDRG1)is a multifunctional gene that has been tentatively reported to have a strong relationship with tumor invasion and migration,however the current molecular role of NDRG1 in CRC remains *** To explore the role of NDRG1 in the development of *** NDRG1 stably over-expressed Caco2 cell line was established by lentiviral infection and NDRG1 knock-out Caco2 cell line was established by CRISPR/***,the mRNA and protein levels of NDRG1 in Caco2 cells after NDRG1 over-expression and knockout were detected by real-time polymerase chain reaction and western *** cell proliferation rate was measured by the cell counting kit-8 method;cell cycle and apoptosis were detected by flow cytometry;invasion and migration ability were detected by the 24-transwell *** NDRG1 over-expression inhibited Caco2 proliferation and the cell cycle could be arrested at the G1/S phase when NDRG1 was over-expressed,while the number of cells in the G2 phase was significantly increased when NDRG1 was knocked *** suggests that NDRG1 inhibited the proliferation of Caco2 cells by arresting the cell cycle in the G1/S *** data also demonstrated that NDRG1 promotes early cell *** and migration of cells were extensively inhibited when NDRG1 was *** NDRG1 inhibits tumor progression in Caco2 cells which may represent a potential novel therapeutic strategy for the treatment of CRC.

关键词： N-myc downstream regulated gene 1 Caco2 Colorectal cancer Tumor progression CRISPR/Cas9 lentivirus infection

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