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STRUCTURE,MOLECULAR WEIGHT AND BIOACTIVITIES OF(1→3)-β-D-GLUCANS AND ITS SULFATED DERIVATIVES FROM FOUR KINDS OF lentinus edodes

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Chinese Journal of Polymer Science 2005年第3期23卷 327-336页

作者： UnursaikhanSurenjav 张俐娜 Xiao-juanXu MeiZhang PeterChiKeungCheung Fan-boZeng DepartmentofChemistry WuhanUniversityWuhan430072China DepartmentofBiological TheChineseUniversityofHongKongHongKongChina TongjiMedicalCollege HuazhongUniversityofScienceandTechnologyWuhan430030China

Lentinan samples,(1→3)-β-D-glucans containing 4.6-15.2 wt% proteins,coded as L-I_1 L-I_2 L-I_3 and L-I_4(L-I)were isolated from four kinds of lentinus *** glucans were treated with acetone to remove the protein in orderto obtain free protein glucans coded as LNP-I_1,LNP-I_***-I_3 and LNP-I_4(LNP-I).The free-protein polysaccharideswere sulfated to give derivatives(S-LNP-I)with degree of substitution(DS)from *** structural features andweight-average molecular weight(M_w)of the samples were investigated by using infrared spectroscopy,elemental analysis,^(13)C-NMR,size exclusion chromatography combined with laser light scattering(SEC-LLS)and *** effects ofstructure and conformation of the polysaccharides on antitumor activities were assayed in vivo(Sarcoma 180 solid tumors)and in vitro(Sarcoma 180,HL-60,MCF-7 and Vero tumors).The results indicated that the predominant species of thesamples L-I and LNP-I in 0.2 mol/L NaCl aqueous solution existed as triple-helical chains with high rigidity and in dimethylsulfoxide(DMSO)as single-flexible ***,the antitumor activities of LNP-I are lower than those of the nativeglucans(L-I),whereas their sulfated derivatives have higher inhibition ratio against Sarcoma 180 than *** resultsreveal that the binding of protein,sulfated modification and the triple helix conformation are important factors in theenhancement of the antitumor activities of polysaccharides on the whole.

关键词： (1→3)-β-D-glucans lentinus edodes Molecular weight Protein Sulfated derivative Antitumor activity.

Fermented lentinus edodes extract containingα-glucan ameliorates concanavalin A-induced autoimmune hepatitis in mice

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Food Science and Human Wellness 2024年第4期13卷 2102-2115页

作者： Xin Hu Dan Cheng Yingxia Zhang Po Li Xiaoping Wu Junsheng Fu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China Mycological Research Center Fujian Agriculture and Forestry UniversityFuzhou 350002China Shanghai Lithy One-Health Group Technology Co. Ltd.Shanghai 200235China

Autoimmune hepatitis(AIH)is a chronic inflammatory liver disease that threatens human health *** aim of this study was to detect the protective effect of a fermented lentinus edodes extract containingα-glucan(FLA),in a concanavalin A(Con A)-induced AIH mouse model and to determine the underlying liver-protective *** results showed that compared with the model group,the level of proinflammatory cytokines in serum of FLA pretreated mice was significantly decreased,and the degree of inflammatory cell infiltration in liver,thymus and spleen was significantly *** polymerase chain reaction,immunohistochemistry,and Western blotting showed that FLA pre-treatment inhibited the Con A-induced apoptosis of hepatocytes by down-regulating the expression of BAX and up-regulating the expression of *** research found that FLA may improve liver injury in mice by activating NRF2 signaling pathway and inhibiting TRAF6/NF-κB signaling ***,FLA may improve liver injury in mice by shifting gut microbial composition to reduce the release of inflammatory cytokines in the serum and prevent the necrosis of ***-regulation of NRF2 signaling pathway,down-regulation of TRAF6/NF-κB signaling pathway,and an increase in the relative abundance of Lactobacillus_johnsonii and Ligilactobacillus_murinus play a protective role in liver.

关键词： Autoimmune hepatitis Concanavalin A lentinus edodes TRAF6/NF-κB NRF2

Mathematical Modeling and Effect of Various Hot-Air Drying on Mushroom(lentinus edodes)

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Journal of Integrative Agriculture 2014年第1期13卷 207-216页

作者： GUO Xiao-hui XIA Chun-yan TAN Yu-rong CHEN Long MING Jian College of Food Science Southwest University Key Laboratory of Food Processing & Technology of Chongqing Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Presevation (Chongqing) Ministry of Agriculture

An experimental study was performed to determine the characteristics and drying process of mushroom （lentinus edodes） by 6 different hot-air drying methods namely isothermal drying, uniform raise drying, non-uniform raise drying, uniform intermittent drying, non-uniform intermittent drying and combined drying. The chemical composition （dry matter, ash, crude protein, crude fat, total sugars, dietary fiber, and energy）, color parameters （L, a＊, b＊, c＊, and h~） and rehydration capacities were determined. Among all the experiments, non-uniform intermittent drying reached a better comprehensive results due to the higher chemical composition, better color quality associated with high bright （26.381＋5.842）, high color tone （73.670＋2.975）, low chroma （13.349a：3.456） as well as the highest rehydration （453.76% weigh of dried body）. Nine kinds of classical mathematical model were used to obtained moisture data and the Midili-kucuk model can be described by the drying process with the coefficient （R2 ranged from 0.99790 to 0.99967）, chi-square （X2 ranged from 0.00003 to 0.00019） and root mean square error （RMSE ranged from 0.000486 to 0.0012367）.

关键词： lentinus edodes hot-air drying mathematical model

Optimization of enzymatic extraction,characterization and bioactivities of Se-polysaccharides from Se-enriched lentinus edodes

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Food Bioscience 2023年第1期51卷 1117-1127页

作者： Haofeng Gu Lei Liang Xin peng Zhu Xinhua Jiang Min Du Zichao Wang School of Modern Agriculture&Biotechnology AnKang UniversityAnkang725000China Ankang Agricultural Science Research Institute Ankang725000China Department of Animal Sciences Washington State UniversityPullmanUSA

Selenium(Se)is a necessary micronutrient,while Se-deficiency is common in most areas *** dietary Se-supplements can address this ***-enriched lentinus edodes are good sources of natural Sepolysaccharides that are promising dietary ***,Se-polysaccharides from Se-enriched lentinus edodes(Se-P-LE)fruit body via enzymatic-extraction(EE)have only been sparsely ***,the EE of Se-P-LE was optimized via Box-Behnken and uniform test,and the physicochemical and bio-functional properties of Se-P-LE were investigated *** results showed that the optimized EE conditions were:extracting temperature of 45℃,pH of 4.5,time of 85 min and a liquid-solid ratio of 20:1 mL/*** addition amount of cellulase,papain and pectinase were 0.80,0.65 and 0.8 g/100 g samples,*** this condition,Se-P-LE yield was 18.16±0.10%,and the Se content of Se-P-LE was 29.51±0.23μg/100 *** of Se-P-LE showed that Se-P-LE were Se-conjugated polysaccharides with pyranoglycan andβ-glycosidic ***-P-LE were composed of 8 monosaccharides with shear-thinning properties and microcrystalline/amorphous *** bioactivities assays showed that Se-P-LE displayed a higher DPPH and hydroxyl radical scavenging rate than ordinary ***,Se-P-LE significantly reduced mRNA expression and levels of pro-inflammation factors(NO,IL-6/1βand TNF-α)caused by LPS in RAW264.7 cell line.

关键词： Extraction Se-polysaccharides Characterization Antioxidant Anti-inflammation lentinus edodes

Identification of phytochemicals from lentinus edodes and Auricularia auricula with UPLC-Q-Exactive Orbitrap MS

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Journal of Future Foods 2022年第3期2卷 253-260页

作者： Lili Cui Yuhang Liu Ming Liu Mengjie Ren Adel F.Ahmed Wenyi Kang National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China Joint International Research Laboratory of Food&Medicine Resource Function Henan ProvinceKaifeng 475004China Medicinal and Aromatic Plants Researches Department Horticulture Research InstituteAgricultural Research CenterGiza 12619Egypt Shenzhen Research Institute of Henan University Shenzhen 518000China

lentinus edodes and Auricularia auricula are the first and second largest edible fungi in China with high nutritional and medicinal *** about phytochemicals of *** and *** mainly focuses on polysaccharides,other phytochemicals are not fully *** of *** and *** were investigated by ultra-high performance liquid chromatography tandem hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive-Orbitrap MS).Twenty-three compounds were identified in ***,mainly including amino acids and analogs,flavonoids,nucleosides and analogs,phenols and ***-nine compounds were identified in ***,mainly including amino acids and analogs,nucleosides and analogs,flavonoids and *** are 9 common ingredients of *** and ***,including 4 amino acids and analogs,2 flavonoids,2 nucleosides and analogs and 1 phenol.

关键词： lentinus edodes Auricularia auricula Phytochemicals UPLC-Q-Exactive-Orbitrap MS

PEG-mediated Transformation of lentinus edodes

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Acta Botanica Sinica 2001年第10期43卷 1089-1092页

作者：孙丽许伟宏蔡华清胡鸢雷林忠平北京大学生命科学学院蛋白质工程和植物基因工程国家重点实验室北京100871

Expression vector p301-bG1 contains a Sw gene and a bialaphos resistance gene both driven by glyceraldehydes-3-phosphate dehydrogenase (GPD) gene promoter isolated from lentinus edodes ( Berk.) Sing. Using p301-bG1, PEG-mediated transformation of protoplast of L. edodes was studied. Mixed with PEG-purified plasmid DNA, the protoplasts of L. edodes were treated with PEG solution and cultured on CYM regeneration plate containing 40 mug/mL bialaphos. Bialaphos-resistant and GUS-positive transformants were obtained using this transformation system. Although the transformation efficiency was relatively low, the protocols release large expenses on expensive instrument and restriction enzymes, providing a simple and economical method for mushroom breeding at the molecular level.

关键词： lentinus edodes transformation GPD promoter gus PEG

香菇[lentinus edodes (Berk.)Sing.]高产优质菌株的选择及其子实体形态发生的细胞学观察

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华中农业大学学报 1982年第2期 28-40页

作者：吕作舟华中农学院应用真菌1978级研究生

为了鉴定野生香菇的经济性状,探讨选育高产优质菌株的较快途径,本试验利用1979年在鄂西山区采集分离得到的35个野生香菇菌株,加上从国内外收集到的香九、7625等10个香菇品种作为试验材料,一共45个菌号,并以引进的7402为对照,采用木屑装... 详细信息

为了鉴定野生香菇的经济性状,探讨选育高产优质菌株的较快途径,本试验利用1979年在鄂西山区采集分离得到的35个野生香菇菌株,加上从国内外收集到的香九、7625等10个香菇品种作为试验材料,一共45个菌号,并以引进的7402为对照,采用木屑装瓶栽培香菇的方法,进行了四次以产量为主的选种出菇试验。结果指出,在45个供试材料中,单产高于7402的有9个菌株,其中包括4个野生香菇菌株(HML44、HML55、HML57、HML58)。这就表明,我国香菇资源丰富,大量采集野生香菇菌株,直接从中选择适应我国的优良品种,或将它们留作育种亲本,是一项快速而切合实际的工作。从结构和功能相联系的观点出发,开展香菇的细胞学研究,可以提高良种选育工作的科学性。试验采用盐酸吉姆萨(HCl Giemsa)染色压片的方法,观察香菇担子中的核行为和三级菌丝体(tertiary mycelium)细胞核的分裂过程。这一初步探讨,可为今后的香菇良种选育工作,以及深入进行其细胞生物学研究提供参考。

关键词：野生香菇经济性状发育程度香菇菌株 lentinus edodes Sing Berk 子实体细胞学生物学形态发生形态建成

香菇(lentinus edodes)谷粒种的研制——Ⅰ制备方法和保存期

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广西科学院学报 1986年第1期 69-73页

作者：林明德农波广西科学院生物研究室

用香菇的液体菌种,接入麦粒基质中,制成香菇麦粒栽培种,可比常规木屑制种缩短将近一半的时间。这种麦粒种经冷库(4～8℃)、室温(10～32℃)、温室(25～28℃)三种温度下保存100天,试验菌株的各种处理均能达到90%以上的萌发率,显著优于室... 详细信息

用香菇的液体菌种,接入麦粒基质中,制成香菇麦粒栽培种,可比常规木屑制种缩短将近一半的时间。这种麦粒种经冷库(4～8℃)、室温(10～32℃)、温室(25～28℃)三种温度下保存100天,试验菌株的各种处理均能达到90%以上的萌发率,显著优于室温保存的木屑常规种的26%的萌发率;贮存228天,除保存于冷库的麦粒种仍能100%萌发,其它温度下保存的麦粒种的萌发率下降程度随保存温度和菌株的不同而不同,但仍优于相同条件下保存、已不能萌发的木屑常规种。

关键词：麦粒种冷库冷藏库香菇属香菇 lentinus edodes 液体菌种谷粒保存期制备方法

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香菇适宜菌株品比较试验

林业科技通讯 2020年第10期 86-87页

作者：叶丙鑫谢淑琴张晶魏镛频张旦李春花定西市农业科学研究院甘肃定西743000

在甘肃中部地区气候条件及生产模式下,以本地香菇(lentinus edodes)子实体分离菌株为对照,通过菌丝生长情况、子实体农艺性状、产量、生物转化率、标准菇比例等方面比较研究,结果表明,菌株‘申香215’综合性状比较优良且适合甘肃中部地... 详细信息

在甘肃中部地区气候条件及生产模式下,以本地香菇(lentinus edodes)子实体分离菌株为对照,通过菌丝生长情况、子实体农艺性状、产量、生物转化率、标准菇比例等方面比较研究,结果表明,菌株‘申香215’综合性状比较优良且适合甘肃中部地区栽培,可作为地方香菇栽培菌株的替代产品。

关键词：香菇 lentinus edodes 品比试验甘肃中部