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Functional traits of poplar leaves and fine roots responses to ozone pollution under soil nitrogen addition

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Journal of Environmental Sciences 2022年第3期34卷 118-131页

作者： Pin Li Rongbin Yin Huimin Zhou Sheng Xu Zhaozhong Feng Research Center for Urban Forestry Key Laboratory for Forest Silviculture and Conservation of Ministry of EducationKey Laboratory for Silviculture and Forest Ecosystem Research in Arid-and Semi-arid Region of State Forestry AdministrationBeijing Forestry UniversityBeijing 100083China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China Institute of Ecology Key Laboratory of Agrometeorology of Jiangsu ProvinceSchool of Applied MeteorologyNanjing University of Information Science and TechnologyNanjing 210044China

Concurrent ground-level ozone(O_(3))pollution and anthropogenic nitrogen(N)deposition can markedly influence dynamics and productivity in *** studies evaluating the functional traits responses of rapid-turnover organs to O_(3) have specifically examined leaves,despite fine roots are another major source of soil carbon and nutrient input in forest *** elevated O_(3) levels impact fine root biomass and biochemistry remains to be *** study was to assess poplar leaf and fine root biomass and biochemistry responses to five different levels of O_(3) pollution,while additionally examining whether four levels of soil N supplementation were sufficient to alter the impact of O_(3) on these two *** O_(3) resulted in a more substantial reduction in fine root biomass than leafbiomass;relative to leaves,more biochemically-resistant components were present within fine root litter,which contained high concentrations of lignin,condensed tannins,and elevated C:N and lignin:N ratios that were associated with slower rates of litter *** contrast,leaves contained more labile components,including nonstructural carbohydrates and N,as well as a higher N:P *** O_(3) significantly reduced labile components and increased biochemically-resistant components in leaves,whereas they had minimal impact on fine root *** suggests that O_(3) pollution has the potential to delay leaf litter decomposition and associated nutrient cycling.N addition largely failed to affect the impact of elevated O_(3) levels on leaves or fine root chemistry,suggesting that soil N supplementation is not a suitable approach to combating the impact of O_(3) pollution on key functional traits of *** results indicate that the significant differences in the responses of leaves and fine roots to O_(3) pollution will result in marked changes in the relative belowground roles of these two litter sources within forest ecosystems,and such chang

关键词： Ozone pollution Nitrogen(N)input Fine roots leaves Functional traits Chemical complexity

Handling the Challenges of Small-Scale Labeled Data and Class Imbalances in Classifying the N and K Statuses of Rubber leaves Using Hyperspectroscopy Techniques

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Plant Phenomics 2024年第2期6卷 281-294页

作者： Wenfeng Hu Weihao Tang Chuang Li Jinjing Wu Hong Liu Chao Wang Xiaochuan Luo Rongnian Tang School of Mechanical and Electrical Engineering Hainan UniversityHaikou 570228China School of Electrical Engineering and Automation Tianjin UniversityTianjin 300072China

The nutritional status of rubber trees(Hevea brasiliensis)is inseparable from the production of natural ***(N)and potassium(K)levels in rubber leaves are 2 crucial criteria that reflect the nutritional status of the rubber *** hyperspectral technology can evaluate N and K statuses in leaves ***,high bias and uncertain results will be generated when using a small size and imbalance dataset to train a spectral estimaion model.A typical solution of laborious long-term nutrient stress and high-intensive data collection deviates from rapid and flexible advantages of hyperspectral ***,a less intensive and streamlined method,remining information from hyperspectral image data,was assessed.

关键词： handling data ersp challenges classifying imbalances labeled leaves rubber small-scale

Preparation, Morphology Transformation and Magnetic Behavior of Co3O4 Nano-leaves

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Chinese Physics Letters 2010年第12期27卷 196-198页

作者：孟令镕陈伟孟陈晋平周和平彭卿 State Key Laboratory of New Ceramics and Fine Processing Department of Chemistry and Department of Materials Science and Engineering Tsinghua University Beijing 100084 Department of Physics Peking University Beijing 100871

A series of cubic phase Co3O4 nano-leaves were prepared via a combined approach of solution reaction and calcination. According to x-ray diffraction and electron microscopy, we find that the Co3 04 grain size increases with calcination temperature. This can induce many gaps in the products. M-T and M-H magnetization measurements reveal the typical antiferromagnetie behavior of nano-leaves. The effective moments of the samples prepared at 300, 400 and 500℃ are 5.6, 5.8 and 5.7μB per formula unit （FU）, respectively, larger than the bulk value of 4.14 μB/FU.

关键词： leaves Microscopy Measurement

Study of the Geometry and Folding Pattern of leaves of Mimosa pudica

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Journal of Bionic Engineering 2007年第1期4卷 19-23页

作者： H. S. Patil Siddharth Vaijapurkar Department of Applied Mechanics S.V. National Institute of Technology Ichchanath Surat Gujarath 395007 India

Many structural and functional properties possessed by plants have great potentials to stimulate new concepts and innovative ideas in the field of biomimetic engineering. The key inputs from biology can be used for creation of efficient and optimized structures. The study of the geometry and folding pattern of leaves of Mimosa pudica, referred as Sensitive Plant, reveals some of the peculiar characteristics during folding and unfolding. When the leaf is touched, it quickly folds its leaflets and pinnae and droops downward at the petiole attachment. With the help of experiments on simulation model, the variations in angle of leaflets and degree of compaction after folding are investigated.

关键词： bionics biomimetics folding geometry leaves Mimosa pudica

Tomato LysM receptor kinase 4 mediates chitin-elicited fungal resistance in both leaves and fruit

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Horticulture Research 2023年第6期10卷 170-181页

作者： Yingfei Ai Qinghong Li Chenying Li Ran Wang Xun Sun Songyu Chen Xin-Zhong Cai Xingjiang Qi Yan Liang State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Department of Plant ProtectionZhejiang UniversityHangzhou310058China Hainan Institute Zhejiang UniversitySanya572025China Zhejiang Xianghu Laboratory Institute of HorticultureZhejiang Academy of Agricultural SciencesHangzhou310021China

Fungal infection is a major cause of crop and fruit *** of chitin,a component of fungal cell walls,endows plants with enhanced fungal ***,we found that mutation of tomato LysM receptor kinase 4(SlLYK4)and chitin elicitor receptor kinase 1(SlCERK1)impaired chitin-induced immune responses in tomato *** with the wild type,sllyk4 and slcerk1 mutant leaves were more susceptible to Botrytis cinerea(gray mold).SlLYK4 extracellular domain showed strong binding affinity to chitin,and the binding of SlLYK4 induced SlLYK4-SlCERK1 ***,qRT–PCR analysis indicated that SlLYK4 was highly expressed in tomato fruit,andβ-GLUCURONIDASE(GUS)expression driven by the SlLYK4 promoter was observed in tomato ***,SlLYK4 overexpression enhanced disease resistance not only in leaves but also in *** study suggests that chitin-mediated immunity plays a role in fruit,providing a possible way to reduce fungal infection-related fruit losses by enhancing the chitin-induced immune responses.

关键词： leaves resistance fungal

Identification of suitable reference genes in leaves and roots of rapeseed (Brassica napus L.) under different nutrient deficiencies

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Journal of Integrative Agriculture 2017年第4期16卷 809-819页

作者： HAN Pei-pei QIN Lu LI Yin-shui LIAO Xiang-sheng XU Zi-xian HU Xiao-jia XIE Li-hua YU Chang-bing WU Yan-feng LIAO Xing Oil Crops Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetics Improvement of Oil Crops Ministry of Agriculture Wuhan 430062 P.R. China

Nutrient deficiency stresses often occur simultaneously in soil. Thus, it＇s necessary to investigate the mechanisms underlying plant responses to multiple stresses through identification of some key stress-responsive genes. Quantitative real-time PCR （qRT-PCR） is essential for detecting the expression of the interested genes, of which the selection of suitable reference genes is a crucial step before qRT-PCR. To date, reliable reference genes to normalize qRT-PCR data under different nutrient deficiencies have not been reported in plants. In this study, expression of ten candidate reference genes was detected in leaves and roots of rapeseed （Brassica napus L.） after implementing different nutrient deficiencies for 14 days. These candidate genes, included two traditionally used reference genes and eight genes selected from an RNA- Seq dataset. Two software packages （GeNorm, NormFinder） were employed to evaluate candidate gene stability. Results showed that VHA-E1 was the highest-ranked gene in leaves of nutrient-deficient rapeseed, while VHA-G1 and UBC21 were most stable in nutrient-deficient roots. When rapeseed leaves and roots were combined, UBC21, HTB1, VHA-G1 and A CT7 were most stable among all samples. To evaluate the stabilities of the highest-ranked genes, the relative expression of two target genes, BnTrxl;1 and BnPhtl;3 Were further determined. The results showed that the relative expression of BnTrxl;1 depended on reference gene selection, suggesting that it＇s necessary to evaluate the stability of reference gene prior to qRT-PCR. This study provides suitable reference genes for gene expression analysis of rapeseed responses to different nutrient deficiencies, which is essential for elucidation of mechanisms underlying rapeseed responses to multiple nutrient deficiency stresses

关键词： reference genes rapeseed (Brassica napus L.) nutrient deficiency leaves roots

Chlorophyll extraction from leaves,needles and microalgae:A kinetic approach

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International Journal of Agricultural and Biological Engineering 2013年第2期6卷 107-115页

作者： Krystian Miazek Stanislaw Ledakowicz Department of Bioprocess Engineering Technical University of Lodz90-924LodzPoland

Currently,there is a strong focus on industrial production of chlorophyll as a natural *** factors are required in the economically feasible process to make chlorophyll production:material with high pigment content and efficient extraction *** this work,extraction of chlorophyll from harvested black locust(Robinia pseudoacacia)leaves,Scots pine(Pinus sylvestris)needles,field sow thistle(Sonchus arvensis)leaves,and green microalga(Chlorella sp.)was *** highest pigment content was detected in Chlorella cells(4.46%)followed by black locust leaves(1.63%),sow thistle leaves(1.48%)and pine needles(0.38%).The chlorophyll extraction rate was the highest for black locust leaves(k=3.59 h^(-1)),sow thistle leaves(k=2.90 h^(-1))and Chlorella cells(k=2.80 h^(-1))with the use of methanol as a *** investigated materials,needles showed higher resistance for chlorophyll extraction(k=0.93 h^(-1))when compared to leaves and *** of extraction kinetic constant were much lower for all materials(0.22-1.12 h^(-1))in the case of using ethanol as a *** locust leaves and Chlorella cells were proved to be the most attractive materials for chlorophyll production.

关键词： chlorophyll extraction leaves needles microalgae kinetic approach

Transcriptomic profiling of sorghum leaves and roots responsive to drought stress at the seedling stage

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Journal of Integrative Agriculture 2019年第9期18卷 1980-1995页

作者： ZHANG Deng-feng ZENG Ting-ru LIU Xu-yang GAO Chen-xi LI Yong-xiang LI Chun-hui SONG Yan-chun SHI Yun-su WANG Tian-yu LI Yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Drought stress affects the growth and productivity of crop plants including *** study the molecular basis of drought tolerance in sorghum,we conducted the transcriptomic profiling of sorghum leaves and roots under drought stress using RNA-Seq method.A total of 510,559,and 3 687 differentially expressed genes(DEGs)in leaves,3 368,5 093,and 4 635 DEGs in roots responding to mild drought,severe drought,and re-watering treatments were identified,*** them,190 common DEGs in leaves and 1 644 common DEGs in roots were responsive to mild drought,severe drought,and re-watering *** Ontology(GO)enrichment analysis revealed that the GO categories related to drought tolerance include terms related to response to stimulus especially response to water deprivation,abscisic acid stimulus,and reactive oxygen *** major transcription factor genes responsive to drought stress include heat stress transcription factor(HSF),ethylene-responsive transcription factor(ERF),Petunia NAM,Arabidopsis ATAF1/2 and CUC2(NAC),WRKY transcription factor(WRKY),homeodomain leucine zipper transcription factor(HD-ZIP),basic helix-loop-helix transcription factor(bHLH),and V-myb myeloblastosis viral oncogene homolog transcription facotr(MYB).Functional protein genes for heat shock protein(HSPs),late-embryogenesis-abundant protein(LEAs),chaperones,aquaporins,and expansins might play important roles in sorghum drought ***,the genomic regions enriched with HSP,expansin,and aquaporin genes responsive to drought stress could be used as powerful targets for improvement of drought tolerance in sorghum and other ***,our results provide a genome-wide analysis of DEGs in sorghum leaves and roots under mild drought,severe drought,and re-watering *** study contributes to a better understanding of the molecular basis of drought tolerance of sorghum and can be useful for crop improvement.

关键词： sorghum(Sorghum bicolor L.) transcriptomic profiling drought leaves roots

Effect of dietary supplementation with mulberry(Morus alba L.) leaves on the growth performance,meat quality and antioxidative capacity of finishing pigs

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Journal of Integrative Agriculture 2019年第1期18卷 143-151页

作者： ZENG Zhu JIANG Jun-jie YU Jie MAO Xiang-bing YU Bing CHEN Dai-wen Key Laboratory for Animal Disease-Resistant Nutrition Ministry of Education/Institute of Animal NutritionSichuan Agricultural University

The present study was conducted to evaluate the effect of dietary mulberry(Morus alba L.) leaves powder(MLP) supplementation on meat quality of finishing pigs.A total of 40 Duroc×Landrace×Yorkshire pigs(initial body weight of(40.5±0.63) kg) were randomly allotted into two treatments,fed either with control diet or 15% MLP diet for 85 *** results showed that MLP diet decreased(P≤0.05) average daily gain(ADG) and increased(P<0.05) feed/gain ratio(F/G) in the finishing and whole *** diet also decreased(P<0.05) carcass weight,dressing percentage,last rib and average backfat ***,MLP diet increased(P<0.05) intramuscular fat(IMF) content,decreased(P<0.05) shear force,cooking loss and drip *** addition,MLP diet increased(P<0.05) total antioxidative capacity,glutathione peroxidase and tended(P<0.10) to increase total superoxide dismutase in *** longissimus thoracis,myosin heavy chain(MyHC) I and IIa mRNA levels were increased(P≤0.05) for MLP *** conclusion,15% MLP supplementation reduced the growth performance and carcass traits,but improved meat quality of finishing pigs possibly through the change of myofiber characteristics,enhancement of antioxidative capacity and increase of IMF.

关键词： mulberry leaves powder meat quality myofibers antioxidation pigs