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A Sandwich-type lateral flow strip Using a Split, Single Aptamer for Point-of-Care Detection of Cocaine

Journal of Analysis and Testing 2022年第2期6卷 120-128页

作者： Le Jing Chong-Yu Xie Qian-Qian Li Hui-Fang Yao Mei-Qing Yang Hui Li Fan Xia Shao-Guang Li State Key Laboratory of Biogeology and Environmental Geology Engineering Research Center of Nano-Geomaterials of Ministry of EducationFaculty of Materials Science and ChemistryChina University of GeosciencesWuhan 430074China Hubei University of Police Wuhan 430034China

The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly ***,it is unlikely to match a pair of specifi c aptamers that can bind a small molecular target(e.g.,cocaine)simultaneously due to the steric *** response,we herein introduced the strategy of“one divides into two”into the construction of sandwich-type lateral fl ow strip assay(LFSA).Specifi cally,we split a single cocaine-recognizing aptamer into two segments,either of which was conjugated with gold nanoparticles(AuNPs)or labeled with biotin,serving as signal probe and capture probe,*** the presence of the target molecule,a ternary sandwich complex comprised of the two halves of the aptamer and the target *** sandwich-type LFSA exhibited an excellent nonlinear logarithmic response in the range from 10μmol/L to 5 mmol/L with R^(2)=*** sensitive on-site detection of cocaine in artifi cial biological samples including urine and sweat was achieved within 15 min,with the visual limit of detection as low as 50μmol/L for urine and 200μmol/L for sweat,and the recovery rates of 83.6-107.4%.

关键词： lateral flow strip Split aptamer Sandwich-type AuNPs Cocaine

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Recombinase polymerase amplification(RPA) combined with lateral flow(LF) strip for equipment-free detection of Cryptosporidium spp. oocysts in dairy cattle feces

Recombinase polymerase amplification(RPA) combined with late...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Yaodong Wu Donghui Zhou Longxian Zhang Wenbin Zheng Jiangang Ma Meng Wang Xingquan Zhu Minjun Xu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences College of Animal Science and Veterinary Medicine Henan Agricultural University College of Animal Science and Technology Jilin Agricultural University College of Animal Science South China Agricultural University

Cryptosporidium is a widespread protozoan parasite that infects a large number of vertebrate animals, resulting in varying degrees of diarrhea or even death. As dairy cattle feces is an important source of Cryptosporidium spp. infection, development of a handy and accurate detection method via its oocysts in dairy cattle feces would be interesting and necessary. We herein developed a quick detecting method using recombinase polymerase amplification(RPA) combined with lateral flow(LF) strip to detect DNA of Cryptosporidium oocysts in dairy cattle feces. The DNA was released by boiled water with 0.1% N-lauroylsarcosine sodium salt(LSS). The established method was proven to be of higher sensitivity than normal PCR amplification with the lowest detection of 0.5 oocyst per reaction, and specificity with no cross reactivity to other common protozoan species in the intestine of dairy cattle. The diagnostic method established herein is simple, rapid, cost-effective, and has potential for further development as a diagnostic kit for the diagnosis of cryptosporidiosis of dairy cattle.

关键词： Cryptosporidium spp N-lauroylsarcosine sodium salt Recombinase Polym erase Amplification(RPA) Dairy cattle lateral flow strip

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