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Fungal Diversity 2015年第2期71卷 201-214页

作者： B.T.Linaldeddu A.Deidda B.Scanu A.Franceschini S.Serra A.Berraf-Tebbal M.Zouaoui Boutiti M.L.Ben Jamâa A.J.L.Phillips Dipartimento di Agraria Sezione di Patologia vegetale ed EntomologiaUniversitàdegli Studi di Sassarivia E.De Nicola 107100 SassariItaly Département des Biotechnologies Facultédes Sciences de la Vie et de la TerreUniversitéSaad Dahleb09000 BlidaAlgeria INRGREF Laboratoire de Gestion et de Valorisation des Ressources ForestièresB.P.N 22080 ArianaTunisia Centro de Recursos Microbiológicos Departamento de Ciências da VidaFaculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

The diversity of Botryosphaeriaceae species associated with“Botryosphaeria dieback”of grapevine was investigated in 18 vineyards in Sardinia,*** isolates obtained from different woody hosts including holm oak,sweet orange and broom bush in Italy,Algeria and Tunisia were also *** and cultural characteristics as well as ITS and EF1-αsequence data were used to identify the fungal ***-eight botryosphaeriaceous isolates were obtained from 113 symptomatic grapevine samples,from which ten species were *** seriata was the dominant species(25%of isolates),followed by Neofusicoccum parvum(21.7%).Two species,Diplodia olivarum and *** are reported for the first time on *** addition,two new species namely lasiodiplodia mediterranea *** grapevine,holm oak and sweet orange and lasiodiplodia exigua *** broom bush are *** artificial inoculation experiments conducted on excised green grapevine shoots and lignified canes as well as holm oak seedlings,*** was shown to be an aggressive pathogen.

关键词： Citrus×sinensis Diplodia lasiodiplodia Neofusicoccum Quercus ilex Retama raetam

lasiodiplodia theobromae激活芒果防御酶基因差异表达的研究

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西南农业学报 2018年第7期31卷 1451-1455页

作者：康浩苏初连梅志栋杨石有刘晓妹蒲金基张贺海南大学热带农林学院海南海口570228 中国热带农业科学院环境与植物保护研究所海南海口571101 澄迈县农业局海南澄迈571900

【目的】为明确lasiodiplodia theobromae侵染芒果组织的过程中对防御酶相关基因的诱导表达情况。【方法】本研究采用实时荧光定量PCR技术(qRT PCR)研究了lasiodiplodia theobromae侵染芒果叶片和成熟果实时Mi POD、Mi PPO、Mi CHI、Mi ... 详细信息

【目的】为明确lasiodiplodia theobromae侵染芒果组织的过程中对防御酶相关基因的诱导表达情况。【方法】本研究采用实时荧光定量PCR技术(qRT PCR)研究了lasiodiplodia theobromae侵染芒果叶片和成熟果实时Mi POD、Mi PPO、Mi CHI、Mi PR1等7个防御酶基因表达情况。【结果】lasiodiplodia theobromae侵染芒果果实后均不同程度的激活芒果抗病防御酶的活性,抗病防御酶相关基因的表达量极显著,均在被侵染36 h后达最大值,基因Mi POD表达量最高为0 h的33倍,Mi PAL为0 h的450倍;侵染叶片后,活性氧相关基因表达量极显著,均在被侵染24 h后达最大值,抗病相关基因中Mi POD和Mi PPO的表达量呈先上升后下降趋势,基因Mi CHI持续高表达,48 h时表达量达到最大值约为0 h的40倍,Mi PR1在接种72 h时出现最高表达量。【结论】研究表明,寄主植物感病后,Mi POD、Mi PPO、Mi CHI、Mi PR1等7个防御酶基因活性增强,增加寄主植物对病原菌的抵抗能力,进而延缓了病原菌在寄主组织内的迅速扩展。

关键词：芒果 lasiodiplodia theobromae 防御酶基因

茶树病害病原菌lasiodiplodia theobromae生物学特性研究

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中国植保导刊 2019年第4期39卷 5-12页

作者：包兴涛李冬雪任亚峰王雪尹桥秀王德炉王勇武娴陈卓贵州大学绿色农药与农业生物工程教育部重点实验室贵州贵阳550025 贵州大学林学院贵阳贵州550025 贵州大学农学院贵阳贵州550025

采用皿内测定方法对贵州省惠水县茶树叶斑病的病原菌lasiodiplodia theobromae进行了病原生物特性研究。结果表明,其病原菌可在SM、PDA、OA和MEA培养基上生长,菌株在MEA培养基上的生长速率显著快于SM培养基、PDA培养基和OA培养基。病原... 详细信息

采用皿内测定方法对贵州省惠水县茶树叶斑病的病原菌lasiodiplodia theobromae进行了病原生物特性研究。结果表明,其病原菌可在SM、PDA、OA和MEA培养基上生长,菌株在MEA培养基上的生长速率显著快于SM培养基、PDA培养基和OA培养基。病原菌适宜在OA培养基上产孢。碳素和氮素营养可影响菌株的生长及菌丝色素的形成。在PDA培养基上,菌株在33℃条件下的生长速度最快,可适应酸性和碱性条件。但在弱碱条件下生长最为迅速。菌株可在PDB培养基中生长,接种菌碟后生长88 h进入稳定期。

关键词：茶树叶部病害 lasiodiplodia theobromae 生物学特性

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一株毛色二孢菌发酵产茉莉酸

食品与发酵工业 2018年第3期44卷 22-29页

作者：董文华郑璞陈鹏程赵路平江南大学生物工程学院工业生物技术教育部重点实验室江苏无锡214122

从实验室保存和自然界分离到的100多株霉菌中筛选到1株高产茉莉酸的毛色二孢菌lasiodiplodiairanensis DWH-2。优化得到发酵培养基组分为:蔗糖50 g/L,NaNO_34.5 g/L,KH_2PO_42.0 g/L,KCl 0.4 g/L,MgSO_4·7H_2O 6 g/L,FeSO_4·7H_2O 0.9 g/L,玉米浆1.5 g/L,微量元素10 ml/L,大豆油10 g/L;发酵初始p H 5.5,装液量100 m L/500 m L,培养温度28℃。在此条件下,发酵7 d,茉莉酸产量可达1 543 mg/L。

关键词：茉莉酸 lasiodiplodia iranensis 发酵培养优化

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一种由可可毛色二孢引起的春芋新病害

植物病理学报 2019年第2期49卷 276-280页

作者：张茜郝媛媛吴雪雍许俊杰龙慧张燕王勇贵州大学农学院贵阳550025 山东省黄河三角洲可持续发展研究院管理中心东营257091 贵阳市花溪区清华中学贵阳550000 临沂大学现代中药研究院临沂276000

可可毛色二孢(lasiodiplodia theobromae(Pat.) Griffon&Maubl.)是由Griffon和Maublanc[1]在对Botryodiplodia theobromae进行订正的基础上而确立,Alves等[2]于2008年对这个种又进行了研究并补充了DNA序列信息。可可毛色二孢是一种重要... 详细信息

关键词： lasiodiplodia pseudotheobromae phylogeny morphology ornamented plants

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茶树叶斑病病原菌可可毛色二孢菌的鉴定

植物病理学报 2019年第6期49卷 857-861页

作者：任亚峰包兴涛李冬雪王勇王德炉宋宝安陈卓贵州大学绿色农药与农业生物工程教育部重点实验室贵阳550025 贵州大学农学院贵阳550025 贵州大学林学院贵阳550025

可可毛色二孢菌(lasiodiplodia theobromae Pat. Griffon & Maubl.)属葡萄座腔菌属(Botryosphaeria)病原菌的无性型,其有性型为Botryosphaeria rhodina(Berk. & M.A. Curtis)Arx[1,2]。该病原菌可导致热带、亚热带地区的植物发生病害,... 详细信息

关键词： tea diseases lasiodiplodia theobromae morphological characteristics pathogenicity phylogenetic analysis

Towards a natural classification of Botryosphaeriales

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Fungal Diversity 2012年第6期57卷 155-216页

作者： Jian-Kui Liu Rungtiwa Phookamsak Mingkhuan Doilom Saowanee Wikee Yan-Mei Li Hiran Ariyawansha Saranyaphat Boonmee Putarak Chomnunti Dong-Qin Dai Jayarama D.Bhat Andrea I.Romero Wen-Ying Zhuang Jutamart Monkai E.B.Gareth Jones Ekachai Chukeatirote Thida Win Ko Ko Yong-Chang Zhao Yong Wang Kevin D.Hyde Institute of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand The Research Institute of Resource Insects Chinese Academy of ForestryKunming 650034China Formerly at Department of Botany Goa UniversityGoa 403206India Prhideb-Conicet Deptomento Cs.BiológicasFacultad de Cs.Exactas y Naturales(UBA)Ciudad UniversitariaPabellón II4to Piso1428 Buenos AiresArgentina State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Institute of Ocean and Earth Sciences(IOES) C308Institute of Postgraduate Studies BuildingUniversity of MalayaKuala Lumpur 50603Malaysia Macrofungi Research Lab Institute of Biotechnology&Germplasmic ResourceYunnan Academy of Agricultural ScienceKunming 650034China Department of Plant Pathology Agriculture CollegeGuizhou UniversityGuiyang 550025China

The type specimens of Auerswaldia,Auerswaldiella,Barriopsis,Botryosphaeria,Leptoguignardia,Melanops,Neodeightonia,Phaeobotryon,Phaeobotryosphaeria,Phyllachorella,Pyrenostigme,Saccharata,Sivanesania,Spencermartinsia and Vestergrenia were examined and fresh specimens of Botryosphaeriales were collected from *** material is used to provide a systematic treatment of Botryosphaeriales based on morphology and *** new genera,Botryobambusa and Cophinforma are introduced and comparedwith existing *** species newto science,Auerswaldia dothiorella,***,Botryosphaeria fusispora and Phaeobotryosphaeria eucalypti,are also described and *** accept 29 genera in Botryosphaeriales,with Macrovalsaria being newly *** the phylogenetic tree,the 114 strains of Botyrosphaeriales included in the analysis cluster into two major clades with 80%,96%and 1.00(MP,ML and BY)support,with Clade A containing the family type of Botryosphaeriaceae,and Clade B containing Phyllosticta,Saccharata and Melanops *** group may represent *** Clade A the taxa analyzed cluster in eight sub-clades(Clades A1-8).Clade A1 comprises three distinct subclusters corresponding to the genera Diplodia(Diplodia Clade),Neodeightonia(Neodeightonia Clade)and lasiodiplodia(lasiodiplodia Clade).Clade A2 clusters into three groups representing Phaeobotryosphaeria(100%),Phaeobotryon(100%)and Barriopsis(94%).Clade A3 incorporates 17 strains that cluster into three well-supported genera(Dothiorella(86%),Spencermartinsia(100%)and Auerswaldia(63%);the position of Macrophomina is not *** A4 is a single lineage(100%)representing the new genus *** A5 is a wellsupported subclade incorporating *** A6 represents the type species of Botryosphaeria,three other Botryosphaeria species and two other genera,Neoscytalidium and Cophinforma *** A7 comprises two Pseudofusicoccum species and Clade A8 has two Aplosporella speci

关键词： Asexual morphs Botryosphaeria Botryosphaeriaceae Classification Diplodia Generic types lasiodiplodia Multi-gene phylogeny New genera Phyllosticta

Gummosis Disease of Mango and Its Control

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Chinese Forestry Science and Technology 2012年第4期11卷 25-32页

作者： UDDINMohammad Sorof FENG Jiaxun HE Xinhua SUNWenli CHENG Qi Biotechnology Resea rch InstituteofChinese Academy ofAgr icultura l Sciences Graduate School ofChinese Academy ofAgricultura l Sciences College of Life Sciences and Technology Guangxi University College ofAgr iculture Guangxi University

Gummosis is the most serious disease of mango all around the world,which is becoming epidemic in different regions of these countries,can infect almost all the commercial and indigenous varieties of mango,and has been regarded as a new serious threat of mango trees at an alarming *** article reviewed causal organisms,current status,future prospects,and management practices of mango gummosis disease.

关键词： mango(Mangifera indica L.) gummosis lasiodiplodia theobromae sudden decline

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楠木枝枯病病原菌鉴定

植物病理学报 2019年第5期49卷 699-704页

作者：岳阳吴利平夏俊勇马良进浙江农林大学林业与生物技术学院临安311300 建德市森林病虫防治检疫站建德311600 淳安县林业局姜家林业中心站淳安311722

楠木为我国传统名贵树种,其木质紧密、耐腐蚀性强,能够驱虫美化环境,是建筑、家具、雕刻的首选木料;同时具有净化空气、吸烟滞尘、涵养水源、固土防沙等功能,有着巨大的经济及生态价值。楠木主要包括润楠属(Machilus Nees)以及楠属(Phoe... 详细信息

楠木为我国传统名贵树种,其木质紧密、耐腐蚀性强,能够驱虫美化环境,是建筑、家具、雕刻的首选木料;同时具有净化空气、吸烟滞尘、涵养水源、固土防沙等功能,有着巨大的经济及生态价值。楠木主要包括润楠属(Machilus Nees)以及楠属(Phoebe Nees)植株,均属于濒危树种,在我国境内主要分布于西南及长江以南地区[1,2]。

关键词： lasiodiplodia pseudotheobromae molecular identification twig blight Phoebe

橡胶木变色菌和霉菌的研究——变色和接种试验

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西部林业科学 1993年第1期29卷 61-64页

作者：赵桂华何文龙宋桢南京林业大学

本文对从变色,发霉橡胶木上分离到的22个分离物进行了变色和接种试验。结果表明,22个分离物都能不同程度的引起橡胶木的变色,其中以 lasiodiplodia theobromae,Dothio-rella major 和 Haplosporella sp.变色最严重。若将它们接种到杨树... 详细信息

本文对从变色,发霉橡胶木上分离到的22个分离物进行了变色和接种试验。结果表明,22个分离物都能不同程度的引起橡胶木的变色,其中以 lasiodiplodia theobromae,Dothio-rella major 和 Haplosporella sp.变色最严重。若将它们接种到杨树枝条上,则能形成典型的溃疡斑,并在其上形成子实体。lasiodiplodia theobromae 的海南分离物接种在杨树枝条上45～55天就能产生大量的分生孢子角。

关键词：橡胶木变色试验 lasiodiplodia theobromae Dothiorella major Haplosporella sp.