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Testis Development and Ultrastructural Features During Spermatogenesis in Cultured Small Yellow Croaker,larimichthys polyactis

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中国海洋大学学报:英文版 2023年第5期22卷 1412-1426页

作者： ZHENG Xuebin WANG Jingqian GAO Xinming DU Chen HOU Congcong XIE Qingping LIU Feng ZHU Junquan LOU Bao Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China Zhejiang Academy of Agricultural Sciences Hangzhou 310021China

The small yellow croaker larimichthys polyactis is an economically important marine fish in Northeast Asia.Currently,its natural resources are threatened by overfishing and environmental pollution.Therefore,research on the reproductive system of the fish is crucial.Here,we studied the testis development and ultrastructural features of spermatogenesis in cultured L.polyactis using anatomical,histological,and ultrastructural techniques.A pair of testes,consisting of a central sperm duct and radial seminiferous lobules,were observed.The reproduction cycle of testes can be divided into stages I–VI.March to May was confirmed as the breeding season for male L.polyactis,while April is the ideal period for artificial breeding.The male L.polyactis can attain sexual maturity within 1 year.The spermatogenesis of L.polyactis comprised spermatogonium,spermatocyte,spermatid,and mature spermatozoon.The morphology of spermatogenic cells changed obviously during spermiogenesis,including nuclear shaping,midpiece and flagellum formation.The mature sperms consist of an ellipsoidal head,a short midpiece,and a long flagellum.The anterior of the head with a kidney-shaped nucleus can be distinguished.The midpiece is located posterior to the head and includes four to six spherical mitochondria.The flagellum has irregular lateral fins.The testis of L.polyactis is an unrestricted lobular type,with cystic spermatogenesis,type II spermiogenesis,and type II spermatozoa.These features are highly similar to those of other Sciaenid species.Our findings provide useful insights into the mechanism underlying testis development and spermatogenesis of L.polyactis,which can facilitate the artificial breeding of this species.

关键词： testis development spermatogenesis larimichthys polyactis histology ultrastructure

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Genetic variation of the small yellow croaker(larimichthys polyactis)inferred from mitochondrial DNA provides novel insight into the fluctuation of resources

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Acta Oceanologica Sinica 2022年第11期41卷 88-95页

作者： Jian Zheng Tianxiang Gao Yunrong Yan Na Song Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Fishery College Zhejiang Ocean UniversityZhoushan 316022China College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China

The small yellow croaker(larimichthys polyactis)belongs to the family Sciaenidae,which is an offshore warm fish species and widely distributed in the western Pacific.In this study,the variation of genetic diversity and genetic differentiation among L.polyactis populations was analyzed by mitochondrial DNA control region.A total of 110 polymorphic sites were checked,which defined 134 haplotypes.High level of haplotype diversity(h=0.993±0.002)was detected in the examined range.Population genetic structure analyse(analysis of molecular variance,Fst)showed there were high gene flow among L.polyactis populations.The result showed that there were relatively high genetic diversity and low genetic differentiation among the Yellow Sea and the East China Sea populations,which can be attributed to diverse habitats,wide distribution range and high mutation rate of control region.Using phylogenetic methods,coalescent analyses(neutrality tests,mismatch distribution analysis,Bayesian skyline analyses)and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidence,we inferred that the genetic make-up of extant populations of L.polyactis was shaped by Pleistocene environmental impacts on the historical demography of this species.Besides,relatively constant genetic diversity and larger effective population size were detected in recent L.polyactis population.The result showed that the fishing policy certainly,such as the summer closed fishing,played a role in protecting resources of L.polyactis.This study can offer a wealth of biological novelties which indicates genetic structure of L.polyactis population and provides the foundation for resources protection and policy setting.

关键词： larimichthys polyactis genetic diversity genetic differentiation mitochondrial DNA control region

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Early life migration and population discrimination of the small yellow croaker larimichthys polyactis from the Yellow Sea: inferences from otolith Sr/Ca ratios

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Journal of Oceanology and Limnology 2022年第2期40卷 818-829页

作者： Dade SONG Ying XIONG Tao JIANG Jian YANG Xiaming ZHONG Jianhua TANG College of Marine Sciences Shanghai Ocean UniversityShanghai 201306China Jiangsu Marine Fisheries Research Institute Nantong 226007China Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of Yangtze River Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi 214081China

The small yellow croaker larimichthys polyactis is a benthic marine fish species of high ecological and commercial importance and is widely distributed in the northwestern Pacifi c Ocean,especially in the Chinese coastal waters of the Bohai,Yellow,and East China Seas.As a highly migratory species,the whole life migration of L.polyactis has been intensively studied.However,knowledge about its early life migration is scarce,and population divisions are inconsistent,limiting the ability of fishery scientists and administrators to evaluate the design and potential benefits of thorough conservation and resource-management strategies.In the present study,otolith Sr/Ca was analyzed to investigate the early migratory patterns and discriminate the populations of L.polyactis in the Yellow Sea,including two spawning groups and one overwintering group.The variation in Sr/Ca ratios of ontogenetic growth zones,including the nucleus(N),larval(L),metamorphosis(M),juvenile(J),and edge(E)zones,was measured by electron probe microanalysis.The variation in Sr/Ca ratios in early developmental growth zones was generally characterized by an evident downward trend from the N to J zone,which suggests that the early migratory pattern of L.polyactis might be from inshore to nearshore water.Canonical discriminant analysis,based on the otolith Sr/Ca ratios of the N,L,M,and J zones,allowed the successful discrimination of the two populations,namely,the northern and southern Yellow Sea groups,whose diff erences were mainly reflected in the L and J zones.Compared with previous studies,the traditional geographic boundaries(34°N)separating these two populations might be moving northward.The application of otolith Sr/Ca ratios based on ontogenetic stage could improve our understanding of the migration and population discrimination of L.polyactis from the Yellow Sea.

关键词： otolith microchemistry larimichthys polyactis early life migration population discrimination the Yellow Sea

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Evidence for the role of KIF17 in fish spermatid reshaping:expression pattern of KIF17 in larimichthys polyactis spermiogenesis

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Journal of Oceanology and Limnology 2021年第6期39卷 2322-2335页

作者： Jingqian WANG Xinming GAO Xuebin ZHENG Chen DU Congcong HOU Qingping XIE Bao LOU Feng LIU Shan JIN Junquan ZHU Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China Zhejiang Academy of Agricultural Sciences Hangzhou 310021China

The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in mammals.However,its role in fish spermiogenesis has not been reported.Here,we aimed to clone full-length kif17 cDNA and determine the molecular characteristics and expression patterns of KIF17 in larimichthys polyactis spermiogenesis.The full-length cDNA of L.polyactis kif17(Lp-kif17)was sequenced and found to contain a 332-bp 5′untranslated region,480-bp 3′untranslated region,and 2433-bp open reading frame encoding 810 amino acids.Bioinformatics analyses showed that L.polyactis KIF17(Lp-KIF17)shared high sequence similarity with homologs in other animals and possessed an N-terminal motor domain with microtubule-binding sites and adenosine triphosphate(ATP)hydrolysis sites,a stalk domain containing two coiled-coil regions,and a C-terminal tail domain.The Lp-kif17 mRNA was widely expressed in various tissues,with the highest level in the brain,followed by that in the testis.Fluorescence in situ hybridization(FISH)analysis revealed that Lp-kif17 was continuously expressed in spermiogenesis,showing that it had potential functions in this process.Using immunofluorescence(IF)analysis,we found that Lp-KIF17 colocalized with tubulin and was transferred from the perinuclear cytoplasm to the side of spermatid where the tail forms during spermiogenesis.These findings suggested that KIF17 is involved in L.polyactis spermiogenesis.In particular,it may participate in nuclear shaping and tail formation by interacting with perinuclear microtubules during spermatid reshaping.In addition to providing evidence for the role of KIF17 in fish spermatid reshaping,this study provides important data for studies of reproductive biology in L.polyactis.

关键词： KIF17 spermiogenesis larimichthys polyactis spermatid reshaping

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