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Parentage Analysis of Dongfang No.2, a Hybrid of a Female Gametophyte Clone of laminaria japonica (laminariales, Phaeophyta) and a Male Clone of L. longissima

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Journal of Ocean University of China 2008年第2期7卷 193-198页

作者： YANG Guanpin LIAO Meijie LI Xiaojie CONG Yizhou QU Shancun WANG Tongyong College of Marine Life Sciences Ocean University of China Qingdao 266003 P. R. China Shandong Oriental Ocean Sci-Tec Co. Ltd. Yantai 264003 P R. China Wendeng Fishery Technique Popularization Station of Shandong Province Wendeng 264400 P R. China

The cultivation of the first filial generation of monoploid gametophyte clones of different laminaria species (hybrid laminaria) is an effective way of utilizing heterozygous vigor (heterosis). A female gametophyte clone of L. japonica and a male gametophyte clone of L. longissima were hybridized, generating Dongfang No.2 hybrid laminaria. The parentage of this hybrid laminaria was determined using AFLP of total DNA, SNP of the ITS region of ribosomal RNA transcription unit and microsatellite DNA variation at two loci. In addition to 167 AFLP bands shared by Dongfang No.2, L. japonica and L. longissima, Dongfang No.2 hybrid laminaria shared another 70 and 55 bands with L. japonica and L. longissima, respectively, which were obviously more than 11 bands shared by L. japonica and L. longissima. Dongfang No.2 held both ‘T’ and ‘C’ at position 847 of the ITS region, while ‘T’ at this position was specific for L. japonica and ‘C’ for L. longissima, respectively. Dongfang No.2 also held the microsatellite DNA alleles of two parents together at two microsatellite DNA marker loci. These observations clearly proved that Dongfang No.2 is a true hybrid of L. japonica and L. longissiuma. Unfortunately, the origin of the chloroplast of Dongfang No.2 was not determined based on the variation of RuBisCo spacer. More sequence variants of both ITS region and RuBisCo spacer were identified in Dongfang No.2 and most of them did not exist in either L. japonica or L. longissima. The unexpected variants may be due to the mutation of ga- metophyte clones occurring during their vegetative amplification.

关键词： hybrid laminaria gametophyte clone heterosis laminaria japonica laminaria longissima

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Genetic Mapping of laminaria japonica and L. Iongissima Using Amplified Fragment Length Polymorphism Markers in a ＂Two-Way Pseudo- Testcross＂ Strategy

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Journal of Integrative Plant Biology 2007年第3期49卷 392-400页

作者： Yuhui Li Yingxia Yang Jidong Liu Xiuliang Wang Tianxiang Gao Delin Duan Ocean University of China Qingdao 266003 China Institute of Oceanology the Chinese Academy of Sciences Qingdao 266071 China Ludong University Yantai 264025 China

With a ＂two-way pseudo-testcross＂ mapping strategy, we applied the amplified fragment length polymorphism （AFLP） markers to construct two moderate density genetic linkage maps for laminaria. The linkage maps were generated from the 60 progenies of the F1 cross family （laminaria iongissima Aresch. × L. Japonica Miyabe） with twenty pairs of primer combinations. Of the 333 polymorphic loci scored in 60 progenies, 173 segregated in a 1：1 ratio, corresponding to DNA polymorphisms heterozygous in a single parent, and the other 58 loci existing in both parents followed a 3：1 Mendelian segregation ratio. Among the loci with 1：1 segregating ratios, 79 loci were ordered in 14 linkage groups （648.6 cM） of the paternal map, and 72 loci were ordered in 14 linkage groups （601.9 cM） of the maternal map. The average density of loci was approximately 1 per 8 cM. To Investigate the homologies between two parental maps, we used 58 loci segregated 3：1 for further analysis, and deduced one homologous linkage group. The linkage data developed in these maps will be useful for detecting loci-controlling commercially important traits for laminaria.

关键词： amplified fragment length polymorphism makers genetic linkage map laminaria japonica laminaria longissima segregation ratio two-way pseudo-testcross strategy

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