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Isolation and identification of novel casein-derived bioactive peptides and potential functions in fermented casein with lactobacillus helveticus

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Food Science and Human Wellness 2019年第2期8卷 156-176页

作者： Mengzhu Fan Tingting Guo Wanru Li Jing Chen Fushuo Li Chao Wang Yi Shi David Xi-an Li Shaohui Zhang School of Agriculture and Biology Shanghai Jiao Tong UniversityChina Zhejiang Go Peptides Life Science and Healthcare Technology Co. Ltd.China School of Systemic Biology and Medicine Shanghai Jiao Tong UniversityChina Zhejiang Panda Dairy Group Co.Ltd. China

The present study here establishes a complete and effective method for isolating,purifying and identifying extracellular and intracellular peptides,and also describes the characters and bioactivities of peptides from fermented casein with lactobacillus *** peptides are much larger in quantity and more complex in composition than extracellular peptides,between which the correlation reveals proteolytic and metabolic *** addition,totally 241 different peptide sequences were identified by Nano LC–MS/MS from casein(212)and lactobacillus helveticus proteins(29).These casein-derived peptides mostly originated from-casein,followed byS1-casein,-casein,andS2-casein,and came from extracell(69)and intracell(143),in which common peptides have a total of ***-four of the identified peptides were previously described as bioactive,including angiotensinconverting enzyme(ACE)-inhibitory,antioxidant,immunomodulating,antimicrobial,DPP-IV inhibitory,antiamnesic and anticancer effects and so *** peptides with the potential of some biological activities are obtained,which were described in previous studies.A total of 47 novel peptides of 5 to 26 amino acids that were not disclosed were *** new sources of natural bioactive peptides may have the very high application value as potential new peptide drugs for treatment human *** product peptide DELQDKIHPF found in both extracell and intracell was quantitatively analyzed using the MRM mode of UPLC-U3Q,23.1 and 9.76 ng/mL,*** quantitative analysis of the potential bioactive peptide may also advance the production of peptide products in the future.

关键词： Bioactive peptide Peptide profiling Fermented casein lactobacillus helveticus

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