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laboratory diagnosis of infectious endophthalmitis

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International Journal of Ophthalmology(English edition) 2011年第1期4卷 100-102页

作者： Wen-Jiang Ma Hong Zhang and Shao-Zhen Zhao Eye Centre Tianjin Medical University Tianjin 300070 China

AIM:To analyze the lab diagnosis and etiology of infectious ***:The medical and microbial records of 36 patients diagnosed with infectious endophthalmitis and 8 patients diagnosed with intraocular lens(IOL)-related inflammation between Nov.1999 and Dec.2009 were retrospectively reviewed for lab diagnosis and ***:The inflammatory cell counts in all aqueous humor specimens from infectious endophthalmitis patients were more than in all aqueous humor specimens from patients with IOL-related *** of the 36 aqueous humor samples(44.4%) and 11 of the 24 vitreous humor samples(45.8%) from infectious endophthalmitis patients showed positive results in smears;while 17 aqueous humor samples(47.2%) and 15 vitreous humor samples(62.5%) from infectious endophthalmitis patients showed positive results in ***:The inflammatory cell count may be an important index for infectious endophthalmitis;while,smears can show etiological information earlier.

关键词： laboratory diagnosis infectious endophthalmitis IOL-related inflammation

Update on laboratory diagnosis of Brucellosis

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Update on laboratory diagnosis of Brucellosis

A Comprehensive Overview of the Paradigm of laboratory Findings in the diagnosis of the Pandemic Coronavirus Disease 2019 (COVID-19) in India

第十二届全国免疫学学术大会

作者： XU Sen ZHENG Yuanqiang SHI Yanchun Inner Mongolia Key laboratory of Molecular Biology Inner Mongolia Medical University

The persistent worldwide prevalence of Brucellosis causes serious public health concerns and economic loss to *** is a multi-system disease with significant variability and the patient’s symptoms are similar to other infectious *** addition,images may be misleading to the ***,laboratory tests are needed for the diagnosis and treatment of *** separation of the pathogen is the gold standard for diagnosis of Brucellosis,but there are some problems such as long culture cycle and low detection rate,requiring highly skilled operator and special level three biological safety *** detection including PCR and RT-PCR requires more professional operator,technologies and *** the two methods are potential biological risks and not suitable for "highthroughput" *** the establishment of test tube agglutination reaction by Wright and Smith,the serological test technology has been rapidly developed and *** there are some false positive and false negative,it has many advantages including simple operation,stable reagent,low biological risk and a uniform set of *** is now widely used in medical institutions and veterinary *** now,there are some methods/techniques such as Coombs’ test,complement fixation test,ELISA,fluorescence polarization assay and so *** search for reliable,discriminatory diagnostic and prognostic markers,especially for monitoring disease evolution,are ongoing.

关键词： Brucellosis laboratory diagnosis serological test culture

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Open Journal of Pathology 2022年第1期12卷 12-21页

作者： Ravi Gautam Anshu Gupta Department of Pathology Swami Dayanand Hospital Delhi India Department of Pathology and In-Charge Emergency laboratory Institute of Human Behavior and Allied Sciences (IHBAS) Delhi India

COVID-19 is a pandemic disease caused by 2019 Novel Coronavirus or SARS-CoV-2. As this disease is highly contagious and rapidly spreading, so all countries have to increase their level of preparedness, alert, and response to identify, manage, and care for new cases of COVID-19;laboratory testing is an integral part of this strategy. Accurate results by laboratory diagnosis are beneficial for public health.

关键词： COVID-19 SARS-CoV-2 laboratory diagnosis Molecular Testing diagnosis Diagnostic Tests

Frequency of Rotavirus among under 5 Children Admitted to Wad Madani Pediatric Teaching Hospital with Diarrhea, Gezira State, Sudan (2021-2022)

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Open Journal of Medical Microbiology 2024年第3期14卷 206-214页

作者： Rehab Ibrahim Ali Babekir Adil Mergani Babiker Bakri Yosif Mohamed Nour Department of Microbiology Faculty of Medical Laboratory Science University of Gezira Wad Medani Sudan National Cancer Institute University of Gezira Wad Medani Sudan Department of Medical Parasitology Faculty of Medical Laboratory Science University of Gezira Wad Medani Sudan

Rotavirus is the leading cause of acute gastroenteritis and responsible for 20% of death in children under 5 years of age approximately 111 million episodes of gastroenteritis due to Rotavirus in under 5 children, which result in 25 million visits to clinic, 2 million hospitalization and 352,000 - 592,000 deaths. Children in the poorest countries account 82% of rotaviruses death. Diarrhea disease are major cause of death and disease among children under five years, a child on average suffers 2 to 3 attacks of diarrhea every year. Stools samples were collected from 196 children suffering from diarrhea and were tested for rotaviruses by immune chromatography test antigen (ICT-Ag), ELISA and RT PCR The data were analyzed using statistical package for the Social Sciences (SPSS). The results showed that prevalence of rotavirus was 26/196 (13.3%) by ICT Ag and ELISA and confirmed by RT-PC R. 87.8% of the children were vaccinated by Rota vaccine, 70% of their mothers not using soap for cleaning the children and thought diarrhea not infectious disease and believed it was caused by teething. In conclusion there was a decrease in prevalence of diarrhea by Rotavirus due to insertion of Rota vaccine in routine vaccination, rotavirus infection mostly occurs in poor family that not able to provide safe water and due to poor sanitation and low education of mother beside that some children suffered from malnutrition, so it recommended to incorporate ELISA as a diagnostic tool in routine diagnosis of rotavirus among children suffered from gastroenteritis.

关键词： Diarrhea Rotavirus Under 5 laboratory diagnosis Sudan

laboratory Characterizations on 2007 Cases of Monoclonal Gammopathies in East China

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Cellular & Molecular Immunology 2008年第4期5卷 293-298页

作者： Hao Wang Chunfang Gao Lingling Xu Zaixing Yang Wenjing Zhao Xiantao Kong Department of laboratory Medicine Changzheng Hospital SecondMilitary Medical University Shanghai 200003 China Department of laboratory Medicine Eastern Hepatobiliary HospitalSecond Military Medical University. Shanghai 200438. China

Monoclonal gammopathies are characterized by the presence of monoclonal immunoglobulin in patients with or without evidence of multiple myeloma （MM）, macroglobulinemia, amyloidosis （AL）, or a related plasma cell proliferative disorder. This study aims to evaluate laboratory diagnostic characters of monoclonal gammopathies and investigates the correlation between monoclonal gammopathies and transforming growth factor β1 （TGFβ1）. Immunofixation electrophoresis （IFE）, serum protein electrophoresis （SPE）, nephelometry and urine light chain ELISA were used for laboratory identification of monoclonal immunoglobulins. Plasma TGFβ1 was detected with double-antibodies ELISA. Lightcycler was used for single nucleotide polymorphism （SNP） analysis. Totally 2,007 cases of monoclonal immunoglobulin （M protein） were identified in 10,682 samples. The isotypes of M protein were IgG type 47.1%, IgA 23.0%, IgM 8.7%, IgD 5.3%, free light chain κ 6.1%, λ 9.8%. In reference to IFE, the coherency of diagnosis was serum light chain ratio （κ/λ） 94.4%, quantitation of Igs 83%, light chain quantitation 80.9%, and urine light chain ratio （κ/λ） 58.0%. Plasma TGFβ1 was elevated significantly compared to normal control. The allelic frequency of codon 10 （C 〉 T） was neither associated with the existence of the M protein nor with the M protein isotype. Monoclonal gammopathies can be identified with the combination of IFE, SPE, Igs quantitaion and urine light chain determination. Although TGFβ1, an important cytokine in immune regulation, was elevated in monoclonal gammopathies, the SNPs in coding region of TGFβ1 gene did not confer susceptibility to the development of monoclonal gammopathies in this study. Cellular ＆ Molecular Immunology. 2008;5（4）： 293-298.

关键词： monoclonal gammopathies laboratory diagnosis TGFβ1 SNP

laboratory response to an infant with suspected measles vaccine associated fever and rash in Sri Lanka

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Infectious Medicine 2023年第1期2卷 57-62页

作者： Buddhini Samaraweera Malsha G.Thrimavithana Lakmini Gunathilake Prabath Gankanda Janitha Nanayakkara Janaki I.Abeynayake The Departement of Virology Teaching Hospital KarapitiyaGalleSri Lanka The Ministry of Heath ColomboSri Lanka The Department of Virology Medical Research InstituteColombo 08Sri Lanka

Background:Measles is a highly contagious *** Lanka(SL)has eliminated the measles in *** coun-try is at risk of importation of measles and there could be vaccine-associated measles like ***,it is important to investigate patients with fever,rash to differentiate the wild-type from vaccine-type exclud-ing other suspected pathogens to direct infection prevention and control *** objective is to describe the laboratory investigation procedure in an immunocompetent child,developed fever,rash following measles containing vaccine in post-measles eliminated period,***:This laboratory based investigation was carried out in National Measles laboratory,*** and throat swab were received from a patient with fever,rash,cough and coryza developed at tenth day of receiving the measles containing live-attenuated *** were tested for measles,rubella,and other relevant pathogens according to the laboratory testing algorithm for an immunocompetent child with fever,rash and flu like ***:Measles vaccine type A,Edmonston-strain virus was detected after sequencing in throat swab and measles IgM and IgG were positive at sixth-week of *** addition,influenza A RNA was detected in throat swab at day-three with detectable parvoB19 IgM in blood sample received at sixth-week of post-onset ***:Measles like illness of this immunocompetent child who received measles containing vaccine could be due to measles vaccine-type A or influenza *** a measles eliminated,resource-limited setting in SL,there should be a well-defined,testing algorithm to exclude prevalent possible pathogens according to epidemiological and clinical information.

关键词： Measles vaccine Vaccine-type virus laboratory diagnosis Sequencing Measles elimination

Knowledge of Covid-19 among Medical Scientists in Nigeria

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Journal of Biosciences and Medicines 2021年第6期9卷 11-25页

作者： Lekan-Agunbiade Titilayo Tosin Agunbiade Olalekan Isaiah Omosebi Funmi Ayomide Ogundare Stephen Olusegun Microbiology laboratory Midland Regional Hospital Portlaoise Ireland Intensive Care Unit. Midland Regional Hospital Portlaoise Ireland Lagos State Health Service Commission Isolo General Hospital Ikeja Nigeria Olabisi Onabanjo University Teaching Hospital Ogun State Nigeria

Background: The impacts of the ongoing COVID-19 pandemic have created a need for constant improvement in the effectiveness and efficiency of laboratory diagnosis to contain the spread, aid the treatment and management of positive cases. Inadequate knowledge of COVD-19 and its laboratory diagnosis among medical scientists is detrimental to the reliability of laboratory results, which are critical in the control, and management of the COVID-19 pandemic. The purposes of this study are to determine the knowledge of COVD-19 and to assess the knowledge of laboratory diagnosis of SARS-CoV-2 among medical scientists. Methodology: An internet-broadcasted and validated questionnaire was used to obtain data from 131 medical scientists in Nigeria. The generated data were analyzed using IBM SPSS Statistics version 25. Results: More than half of respondents had good general knowledge and causes (52%), mode of transmission (52.7%), and symptoms (54.2%) of COVID-19. However, only a few (laboratory diagnosis of COVID-19 and Coronavirus detection steps in the right order (45%). Surprisingly, age (F-ratio = 2.729 p = 0.032), gender (χ2 = 4.173;p = 0.041) and level at work (F-ratio = 3.552, p = 0.016) have significant effects on the knowledge of COVID-19 and knowledge of laboratory diagnosis of SARS-CoV-2 among the study participants. Conclusion: There is a need for improvement in the knowledge of COVID-19 diagnosis through relevant work level (work experience) and gender-based training as well as continuous professional development programs for medical scientists in Nigeria.

关键词： COVID-19 Coronavirus Disease Knowledge laboratory diagnosis Medical Scientists

Isolation and Identification of Salmonella pullorum

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Agricultural Science & Technology 2012年第3期13卷 661-663,668页

作者：安辉张大华陈小玲徐福洲贺云霞黄秀芬李桂萍孙惠玲北京市农林科学院畜牧兽医研究所北京100097 内蒙古民族大学生命科学学院内蒙古通辽028000 山东省潍坊市寒亭区畜牧兽医局山东潍坊261100

[Objective] The paper was to explore the cause of disease with symptoms of swelling joint, blanc-loose stool and few deaths, so as to provide suggestions and effective measures for diagnosis and control of the disease in chicken farm. [Method] The diseased chick samples were collected for bacteriological examination. Through bacterial isolation culture, PCR identification, biochemical test and animal test, 5 strains of S. pullorum were identified. [Result] When the isolates were inoculated into SPF chicks, the same disease cases with natural infection could be reproduced. [Conclusion] S. pullorum is the major cause of the disease in chicken farm.

关键词： Pullorum disease Salmonell pullorum laboratory diagnosis

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临床检验指标在新冠诊断及预后中的应用

临床检验指标在新冠诊断及预后中的应用