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Comparative effects of plant growth regulators on leaf and stem explants of labisia pumila var. alata

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 621-631页

作者： Anna Pick Kiong LING Kinn Poay TAN Sobri HUSSEIN Division of Human Biology International Medical University Department of Science Faculty of Engineering and Science Universiti Tunku Abdul Rahman Agrotechnology and Bioscience Division Malaysian Nuclear Agency

Objective: labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.

关键词： Auxin Cytokinin labisia pumila In vitro propagation Plant growth regulators

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Impedance analysis of labisia pumila plant water status

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Information Processing in Agriculture 2015年第3期2卷 161-168页

作者： Diyana Jamaludin Samsuzana Abd Aziz Desa Ahmad Hawa Z.E.Jaafar Department of Biological and Agricultural Engineering Faculty of EngineeringUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Crop Science Faculty of AgricultureUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

labisia pumila(Kacip fatimah)is a popular medicinal plant in *** constituents of this plant have been reported to possess anticancer,antioxidant and anti-inflammatory *** growth and production of *** is greatly influenced by the plant water *** techniques to measure plant water status are generally based on the plant soil moisture,which apparently did not indicate the real water content inside the *** are other techniques to measure directly on the plant such as based on leaf water potential(LWP)and relative water content(RWC).However,these techniques are destructive and time *** this study,four levels of evapotranspiration replacement(ER)treatment which were 100%ER,75%ER,50%ER and 25%ER was applied to 30 polybags of *** *** plant water status was measured using an impedance spectroscopy technique.A pair of electrocardiogram(ECG)electrode connected to an impedance analyzer board was used to measure the impedance value of the leaf samples *** water status parameters such as LWP,RWC,volumetric moisture content(VMC),and leaf thickness were measured using standard *** results show that after 20 weeks of treatment,25%ER had the highest impedance value ranged from 0.10 MX to 0.15 MX at the frequency of 70–100 *** resistance of 100%ER at 20 weeks of treatment increased from 0.70 kX to 1.23 kX as the reactance decreased from 0.51 kX to 0.28 ***,the resistance of 25%ER increased from 1 kX to 1.10 kX as the reactance decreased from 0.88 kX to 0.83 *** polynomial regression of impedance measurements with plant water status parameters(VMC,leaf thickness,LWP and RWC)shows that LWP and RWC had the highest R2(0.78,0.73).The results show that impedance measurement technique is auspicious to evaluate plant water status.

关键词： Impedance spectroscopy Plant water status labisia pumila Impedance Reactance Resistance

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