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电信科学 2000年第11期16卷 62-64页

作者：何玮高阳圣思园信息技术有限公司北京100086

1 概述要留住您的客户,尤其是大客户(LC),必须提供更好的服务.客户关系管理是改善企业与用户关系的有效手段.实施客户关系管理解决方案,不仅需要采用各种不同的软硬件平台,还要进行大量的定制工作.

关键词：大客户管理系统客户关系管理 lcms

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JAVA反射技术在lcms中的应用研究

华北电力大学学报（自然科学版） 2006年第5期33卷 89-92页

作者：刘兆宏高芳郑莉成都东软信息学院四川成都611844 河北大学电子信息工程学院河北保定071002 清华大学计算机科学与技术系北京100084

在lcms系统中基于Java反射技术实现了开放的、通用的数据库操作组件。采用UML语言进行分析、设计,给出了具体的实现方案。通过利用缓存技术有效地提高了该组件的运行效率。

关键词： Java反射 lcms 接口

lcms检测维生素的室内质控血清制备及可行性研究

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生物医学 2022年第2期12卷 66-72页

作者：闻平李曌宇荀丽华白文燕郑应曼苏州豪思医学检验实验室江苏苏州

目的:研制稳定的维生素质控血清,用于实验室内部维生素检测的质量控制。方法:准确称取一定量的维生素A、25-羟基维生素D2、25-羟基维生素D3、维生素E和维生素K1溶于适量的二甲基亚砜中成为母液,适量添加至基质血清中,形成高、低质控血... 详细信息

目的:研制稳定的维生素质控血清,用于实验室内部维生素检测的质量控制。方法:准确称取一定量的维生素A、25-羟基维生素D2、25-羟基维生素D3、维生素E和维生素K1溶于适量的二甲基亚砜中成为母液,适量添加至基质血清中,形成高、低质控血清。分装成1 ml/支,分别贮存在4℃、−20℃和−80℃环境中,以观察其在不同贮存条件下的稳定性和均一性。结果:4℃保存的质控血清,从第4天起维生素K1值迅速下降,其他维生素(A、D2、D3和E)在一周后检测值也明显下降,−20℃和−80℃保存的质控血清中几种维生素的检测值都均可稳定1年以上;均一性检测表明样品间无显著性差异(F值 0.05),均一性良好。结论:本研究制备的质控血清具有良好的均一性和稳定性,可用于实验室内部维生素检测的质量控制。

关键词：维生素质控血清稳定性均一性可行性 lcms

基于lcms的高校校企合作数字化学习平台研究

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价值工程 2013年第24期32卷 195-197页

作者：关凤岩黑龙江科技大学教务处哈尔滨150022

本文根据学习支持服务的思想,结合校企合作办学的教育教学与学生管理实际需要,构建基于lcms学习内容系统的跨时空的数字化学习平台,平台原型采用开源Moodle的框架完成了满足校企合作办学各类专业课程学习、专业建设、毕业设计以及实践... 详细信息

本文根据学习支持服务的思想,结合校企合作办学的教育教学与学生管理实际需要,构建基于lcms学习内容系统的跨时空的数字化学习平台,平台原型采用开源Moodle的框架完成了满足校企合作办学各类专业课程学习、专业建设、毕业设计以及实践课程的数字化学习资源与交互活动的设计与实现。

关键词：校企合作数字化学习 lcms Moodle

几款开源学习内容管理系统(lcms)的应用比较

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价值工程 2011年第13期30卷 156-157页

作者：任锁平刘瑞儒刘舰付妮娜余芬延安大学教育科学学院延安716000

文章借助EduTools网站提供的开源学习内容管理系统,并通过实际安装和使用进行比较,从四个方面作一分析,并结合网络教育和网络课程的实际情况提出选择策略,以其帮助教师合理的选择开源学习内容管理系统。

关键词：开源学习内容管理系统 lcms 网络课程

Profiling the metabolites of astrapterocarpan in rat hepatic 9000g supernatant

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Chinese Journal of Natural Medicines 2019年第11期17卷 842-857页

作者： ZHANG Ya-Zhou XU Feng DONG Jing LIANG Jing HASHI Yuki LIU Guang-Xue LI Yao-Li SHANG Ming-Ying WANG Xuan CAI Shao-Qing State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 10000China School of Pharmaceutical Sciences Guizhou UniversityGuiyang 550000China Shimadzu China MS Center Beijing 10000China

Astrapterocarpan(AP) is a bioactive constituent of Astragali Radix and was selected as a model compound for investigating the in vitro metabolism of pterocarpans in this study. Its in vitro metabolism was conducted by incubation with rat hepatic 9000 g supernatant(S9) in the presence of an NADPH-generating system. At first, four compounds were isolated and their structures were elucidated as 6 a-hydroxy-AP(M1), astrametabolin I [M2, 1 a-hydroxy-9, 10-dimethoxy-pterocarp-1(2), 4-diene-3-one], 9-demethyl-AP(M3, nissolin) and 4-methoxy-astraisoflavan(M4, 7, 2’-dihydroxy-4, 3’, 4’-trimethoxy-isoflavan) on the basis of NMR data, respectively. Among them, M1, M2 and M4 were new compounds. Next, the metabolite profile of AP in rat hepatic S9 was obtained via HPLC-DAD-ESI-IT-TOF-MSn, and 40 new metabolites were tentatively identified. These newly identified metabolites included 9 monohydroxylated metabolites, 1 demethylated metabolite, 7 demethylated and monohydroxylated metabolites, 4 dihydroxylated metabolites, 1 hydration metabolite, 1 didemethylated metabolite, 2 glucosylated metabolites, 1 monohydroxylated and dehydrogenated metabolite, 2 monohydroxylated and demethylated and dehydrogenated metabolites, 2 dimerized metabolites, 3 dimerized and monohydroxylated metabolites, 2 dimerized and didemethylated metabolites, and 5 dimerized and demethylated metabolites. Finally, the major metabolic reactions of AP in rat hepatic S9 were summarized and found to be hydroxylation, demethylation, dimerization, hydration, and dehydrogenation. More importantly, the biotransformation from AP to M2 and the dimerization of AP by incubation with hepatic S9 were reported for the first time. In conclusion, this is the first report on the metabolism of a pure pterocarpan in animal tissues, and these findings will provide a solid basis for further studies on the metabolism of other pterocarpans.

关键词： Pterocarpan Astragali Radix lcms Hepatic S9 Metabolism

衍生化-液质联用法测定噁拉戈利钠中2-氟-3-甲氧基苯硼酸

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上海医药 2022年第15期43卷 54-57,77页

作者：胡素招钱暮霜上海奥博生物医药股份有限公司上海201203

建立了衍生化-液质联用法来检测噁拉戈利钠中的2-氟-3-甲氧基苯硼酸的含量。以N-甲基亚氨基二乙酸为衍生剂,在100℃加热1 h进行衍生,液相色谱采用梯度洗脱模式在Agilent Zorbax Eclipse XDB-C_(18)色谱柱上运行,以甲酸铵水溶液(pH 4.0,1... 详细信息

建立了衍生化-液质联用法来检测噁拉戈利钠中的2-氟-3-甲氧基苯硼酸的含量。以N-甲基亚氨基二乙酸为衍生剂,在100℃加热1 h进行衍生,液相色谱采用梯度洗脱模式在Agilent Zorbax Eclipse XDB-C_(18)色谱柱上运行,以甲酸铵水溶液(pH 4.0,10 mmol/L)作流动相A和乙腈作流动相B,柱温为40℃,进样体积为3μL,流速为0.6 mL/min。在电喷雾(ESI)正离子模式和选择离子监测(SIM+)m/z 299.1下进行质谱分析。检出限和定量限分别为8.1 ng/mL和24.3 ng/mL。本方法在24.3~121.6 ng/mL的浓度范围内线性关系良好,回收率范围为90.4%~106.5%。该方法适用于噁拉戈利钠中痕量水平的2-氟-3-甲氧基苯硼酸分析,方法简单、准确、重现性好。

关键词：噁拉戈利钠 lcms 衍生化 N-甲基亚氨基二乙酸

Application of analytical instruments in pharmaceutical analysis

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Journal of Pharmaceutical Analysis 2013年第2期3卷 I0001-I0003,F0003页

lcms-8040 incorporates newly improved ion optics and collision cell technology to provide higher multiple reaction monitoring （MRM） sensitivity. These improvements also yield higher sensitivity for scan mode measure... 详细信息

关键词： lcms MRM

Application of analytical instruments in pharmaceutical analysis

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Journal of Pharmaceutical Analysis 2013年第2期 144-147页

lcms-8040 lcms-8040 incorporates newly improvedion optics and collision cell technology to provide higher multiple reaction monitoring(MRM) *** improvements also yield higher sensitivity for scan mode measurements,exp... 详细信息

关键词： lcms

Determination of Calcineurin Inhibitors in Dried Blood Spots from Kidney Transplant Recipients

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American Journal of Analytical Chemistry 2013年第10期4卷 27-35页

作者： Lars Wilhelm Martin Nitschke Markus Meier Reinhard Vonthein Jan Kramer Institute of Medical Biometry and Statistics University of Lübeck Lübeck Germany LADR GmbH Medizinisches Versorgungszentrum Dr. Kramer und Kollegen Geesthacht Germany LADR GmbH Medizinisches Versorgungszentrum Dr. Kramer und Kollegen Geesthacht Germany Medical Clinic I Transplant Center University Hospital of Schleswig-Holstein Lübeck Germany Medical Clinic I Transplant Center University Hospital of Schleswig-Holstein Lübeck Germany Medical Clinic I Transplant Center University Hospital of Schleswig-Holstein Lübeck Germany Nephrology Center Reinbek and Geesthacht Reinbek Germany

Background: Determination of cyclosporine A (CsA) and tacrolimus (Tac) in dried blood spots (DBS) could enable drug monitoring in transplanted patients without the necessity of having to take venous blood samples. Therefore, we have developed a method for quantitative determination of calcineurin inhibitors (CNI) by liquid-chromatography-tandem mass spectrometry (lcms). Methods: In a study with 68 kidney transplant recipients (KTR, 34 CsA, 34 Tac), we tested the clinical application of lcms monitoring in DBS in comparison to lcms in whole blood. Results: The measuring range is proven for 27.33 to 1345 ng/ml for CsA and for 1.63 to 39.7 ng/ml for Tac. The requirements for clinical chemical analyses for precision and accuracy are complied with. Stability is documented for a period of 14 days. The study showed the following deviations from lcms in whole blood for determination of CsA and Tac in DBS after introducing a correction factor by the haematocrit (Hct) value (CsA trough level: mean = 4.7%, ±1.96 standard deviation (SD) -52.1% to 61.4%, N = 96;CsA peak level: mean = 7.3%, ±1.96 SD -39.7% to 54.4%, N = 95;Tac trough level: mean = -0.5%, ±1.96 SD -76.4% to 75.3%, N = 88;Tac peak level: mean = 3.9%, ±1.96 SD -80.1% to 88.7%, N = 92). Conclusions: Our data show comparable results with the reference method by means of lcms in whole blood. Therefore, DBS of KTR for determination of CNI levels could be transported on filter cards by mail to the respective laboratory resistant to breakage and the hazard of infection.

关键词： Cyclosporine A Tacrolimus lcms DBS Immunochemistry Kidney Transplant