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实时浊度lamp法检测豆制品中转基因成分

食品与发酵工业 2012年第8期38卷 150-156页

作者：叶蕾沈会平闫鹤李琳石磊华南理工大学轻工与食品学院广东广州510641

通过设计转基因大豆内源基因(Lectin)、外源基因NOS和2个转基因大豆品系(GTS 40-3-2,MON89788)特异性lamp引物,建立快速检测转基因大豆以及鉴定转基因大豆品系的lamp体系,并选取国内豆制品利用CTAB法进行DNA提取,并针对上述基因进行PCR... 详细信息

通过设计转基因大豆内源基因(Lectin)、外源基因NOS和2个转基因大豆品系(GTS 40-3-2,MON89788)特异性lamp引物,建立快速检测转基因大豆以及鉴定转基因大豆品系的lamp体系,并选取国内豆制品利用CTAB法进行DNA提取,并针对上述基因进行PCR和lamp扩增的对比,建立起了检测大豆制品中转基因成分的定性的lamp方法。结果表明:4个lamp方法均有较好的特异性,除了NOS、GTS 40-3-2的检测限为0.1%外,其他lamp方法的灵敏度为0.01%,均能满足实际检测要求;在实时浊度lamp法检测大豆制品转基因成分的鉴定中,传统的CTAB法能成功提取大豆粗加工制品的DNA,且DNA纯度能够进行核酸扩增反应。利用转基因外引物进行的普通PCR结果与实时浊度lamp结果是一致的,说明建立的转基因实时浊度lamp检测方法有很好的准确性。

关键词： lamp 豆制品转基因

lamp(环介导等温扩增)的原理及在寄生虫检测上的应用

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中国人兽共患病学报 2011年第10期27卷 935-939页

作者：史亚东赵子方朱丹张龙现宁长申菅复春河南农业大学牧医工程学院郑州450002

2000年,Notomi等[1]建立了一种快速、简单、灵敏、特异并且低成本的核酸扩增方法——环介导等温扩增法（lamp）,这种新颖的核酸扩增方法具有许多优势[2-4]：（1）扩增效率极高,60min内扩增产物可达到靶基因的109～1010倍;（2）操作简单,

关键词：环介导等温扩增 lamp 寄生虫应用检测扩增方法扩增效率扩增产物

Study on Identification of Salmonella in Textiles with lamp-MALDI TOF MS

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口岸卫生控制 2019年第4期24卷 17-26页

作者： Li Ke Guo Huiqing Wang Yongliang Zhengzhou Customs Technology Center

Objectives To establish a rapid analytical procedure for salmonella in textiles by lamp in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteristic,a set of lamp primer for salmonella is designed. If the amplification result is suspicious or positive,the strains are separated and the determination is rapidly performed through MALDI TOF MS technique. Methods About 70 reference strains closely related to the textiles and public health are selected to perform the specificity study(Salmonella enterica,Salmonella tyhimurium,Salmonella choleraesuis,Salmonella paratyphi A etc.);The bacterium solution of salmonella is diluted to different gradients for the sensitivity study. Results The results show this method is appropriately specific and sensitive,the limit of detection up to 4.6 CFU/mL. Conclusions The procedure is a new technique to detect salmonella in textiles.

关键词： Salmonella InvA Gene lamp MALDI TOF MS

Loop-Mediated Isothermal Amplification (lamp) for the Detection of <i>Listeria monocytogenes</i>and Major Pathogenic Serotypes

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American Journal of Analytical Chemistry 2014年第16期5卷 1057-1064页

作者： Ana Paula Rocha da Costa Mariana de Lira Nunes Carina Lucena Mendes-Marques Alzira Maria Paiva de Almeida Nilma Cintra Leal Departamento de Microbiologia Centro de Pesquisas Aggeu Magalh&atilde es FIOCRUZ-PE Recife Brazil

Rapid identification and characterization of Listeria monocytogenes are required for the food industry, epidemiological studies, and disease prevention and control. However, typing procedures are labor-intensive and time-consuming, and they require technical expertise, a panel of sera and reference culture strains or sophisticated and expensive equipment. To improve upon traditional diagnostic methods for L. monocytogenes we developed and evaluated an efficient procedure for the specific identification of L. monocytogenes and the major pathogenic serotypes of the species based on loop-mediated isothermal amplification (lamp). Four individual reactions were designed using primers targeting any L. monocytogenes serotypes (lamp-AS) and the 1/2a (lamp-1/2a), 1/2b (lamp-1/2b), and 4b (lamp-4b) serotypes. The procedure distinguished L. monocytogenes from closely genetically related species and the targeted serotypes. Cross-reactivity with a few rare serotypes isolated from food or clinical samples did not impair the usefulness of the procedure. Thus, our approach constitutes a fast, easy and low-cost alternative for L. monocytogenes diagnosis and serotyping and may be useful for surveillance and epidemiological investigation programs.

关键词： Listeria monocytogenes Diagnosis Serotypes Identification lamp

Effect of Xenon Arc lamp Irradiation on Properties of Polymethyl Methacrylate for Aviation

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Open Journal of Organic Polymer Materials 2015年第1期5卷 23-29页

作者： Jiangyan Meng Yunying Wang Key Laboratory of Nondestructive Testing Nanchang Hangkong University Ministry of Education Nanchang China

YB-2 aviation polymethyl methacrylate (PMMA) is irradiated in a xenon arc lamp weather resistance test chamber for 1620 hours. The tensile strength, light transmittance, surface morphology, relative molecular mass, infrared absorption spectrum and glass transition temperature (Tg) of PMMA exposed in xenon arc lamp for different durations are tested and characterized by universal testing machine, optical haze instrument, scanning electronic microscopy (SEM), gel permeation chromatograph (GPC), fourier transform infrared spectrometer (FT-IR) and differential scanning calorimetry (DSC), so as to comprehensively analyze the influence of xenon arc lamp irradiation on the performance of PMMA. The results reveal that under the effect of 1620 hours xenon arc lamp irradiation and periodic spraying water, the light transmittance and glass transition temperature do not change significantly, and no new chemical group is produced. After irradiated 360 hours, tiny cracks occur in the surface of PMMA, indicating that they occur at a certain degree of degradation, meanwhile, the main chain may be broken and the relative molecular mass of surface of the material decreases. After exposure of 720 hours, the tensile strength decreases about 30%.

关键词： Aviation Perspex Xenon Arc lamp Irradiation Relative Molecular Mass Surface Morphology Gel Permeation Chromatograph

HCFL lamp Only Lights Up under Coexistence of Cathode and Anode in Ar Gas Space for Half Cycle of AC Driving Circuit

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Journal of Power and Energy Engineering 2019年第2期7卷 1-13页

作者： Lyuji Ozawa Beijing China

Vacuum space between Ar atoms in unlighted HCFL lamps is an electric insulator, because vacuum fills up with strong negative electric field from orbital electrons in 3p6 electron shell of Ar atoms. Vacuum space in lighted FL lamps changes to the neutral vacuum that provides a superconductive vacuum for moving electrons at above room temperature. The complications of lighting mechanisms of HCFL lamps for more than 80 years have clearly solved with coexistence of disparate external and internal electric circuits for each half cycle. External electric circuit acts as two roles. One helps for formation of internal electric circuit in Ar gas space by electric field. Other picks up induced voltages from capacitor CFL. HCFL lamp only lights up with moving electrons in internal DC driving circuit. Electrons in HCFL lamp only move from cathode to anode, which respectively have negative and positive potentials against grand (V = 0), and which are formed with volumes of heated corona light (4G) at around W-filament metal electrodes with a help of heated BaO particles. The HCFL lamp that emits thermoelectrons is a false story. Here we have totally revised the fundamentals of the lighting mechanism of the established HCFL lamps for last 80 years.

关键词： FL lamp Cathode and Anode Superconductive Vacuum Operation Life

Non Specific Amplification with the lamp Technique in the Diagnosis of Tuberculosis in Sri Lankan Settings

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Journal of Tuberculosis Research 2014年第4期2卷 168-172页

作者： K. D. Senarath R. B. Usgodaarachchi V. Navaratne A. Nagahawatte C. D. Wijayarathna J. Alvitigala C. L. Goonasekara Faculty of Medicine General Sir John Kotelawala Defence University Ratmalana Sri Lanka Department of Chemistry University of Colombo Colombo 03 Sri Lanka Faculty of Medicine University of Ruhuna Galle Sri Lanka Central Chest Clinic and Hospital Welisara Sri Lanka

Background: Tuberculosis (TB) remains a burden to Sri Lanka, where the incidence of the disease has been increasing over the past decade. The lack of early and accurate detection of the disease has been the main obstacle to its control. Microscopy or the culturing of mycobacteria from clinical samples is the most commonly used TB diagnostic tools in Sri Lanka. All these methods have their own limitations. Alternative diagnostic methods are therefore of high importance. Objectives: In this study, an attempt was made to validate loop mediated isothermal amplification (lamp), which specifically amplifies a DNA sequence very rapidly at a low cost with limited resources. Methods: Crude DNA extractions of fifty culture isolates prepared from sputum samples, which were collected from patients with suspected TB extracts, were subjected to three separate lamp assays. One assay was specific for 16S ribosomal RNA (16S rRNA) gene in genus Mycobacterium, and could detect the bacteria up to the genus level. The other two contained MTB specific primers targeting rimM or gyrB gene sequences in Mycobacterium tuberculosis (MTB), which enabled detection up to the species level. The sensitivity and specificity of the lamp assays in the identification of mycobacteria or MTB were compared to microscopy and culture techniques. Results: Forty three out of the 47 Mycobacterium cultures were Mycobacterium-positive for lamp assays with universal primers indicating a sensitivity of 92% in identifying Mycobacterium genus. However, thirteen out of 14 culture negatives were also positive with lamp assays, which showed a specificity of only 7% in identifying MTB. The results suggested a high percentage of false positives by lamp assays as compared to culture. Based on the colour changing of ZYBR Green dye and gel electrophoresis of the lamp-amplified product, the detection of a non-specific amplification, even in the absence of target DNA, was recurrently observed. The result was the same even aft

关键词： Tuberculosis lamp Assay Nonspecific Amplification

Daqin lamp-Trade of the Indian Ocean in Late Antiquity

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World History Studies 2018年第2期5卷 28-56页

作者：韩雪飞林英 Sun Yat-Sen University GuangzhouGuangDong ProvinceP.R.China

This paper examines the early Byzantine bronze lamp found in Southeast Asia(Pong Tuk,Thailand)and in southern China that documented in Yin Ju’s ode in 281 *** on these archaeological and written evidences,the authors discuss that the new knowledge on Daqin *** Roman Empire in Chinese sources after the third century was acquired via the Funan *** rise of Funan and its ability to expand trade with India and even Mediterranean world after the Third-Century Crisis in Rome indicated change of trading mode in Indian Ocean in the age of Late *** rise of regional kingdoms from East to West and the spread of new world religions along the sea route were two key factors to start a new type of prosperity in the Indian Ocean trading network.

关键词： Daqin the South Sea Funan early Byzantine Bronze lamp Trade of the Indian Ocean in Late Antiquity

柑橘溃疡病菌的普通lamp及快速lamp检测方法的建立

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植物保护 2013年第3期39卷 95-101页

作者：张仑殷幼平吴瑜佳王中康重庆大学生物工程学院基因工程研究中心重庆400030

建立柑橘溃疡病菌的普通lamp和快速lamp检测方法,使其能应用于基层检验检疫部门对病害的快速检测。利用柑橘溃疡病菌基因组特有的保守区域设计lamp引物,通过优化反应条件,建立柑橘溃疡病菌的普通lamp检测体系;在普通lamp引物的基础上设... 详细信息

建立柑橘溃疡病菌的普通lamp和快速lamp检测方法,使其能应用于基层检验检疫部门对病害的快速检测。利用柑橘溃疡病菌基因组特有的保守区域设计lamp引物,通过优化反应条件,建立柑橘溃疡病菌的普通lamp检测体系;在普通lamp引物的基础上设计一对环引物,建立柑橘溃疡病菌的快速lamp检测体系,并以多种参比菌DNA以及健康柑橘叶片基因组DNA为模板对普通lamp和快速lamp检测体系的特异性进行了验证,利用柑橘溃疡病菌菌液和DNA溶液梯度稀释液对普通lamp和快速lamp检测体系的灵敏度进行了验证。普通lamp检测体系菌体和DNA检测灵敏度分别达到了2.25×104cfu和2.03×10-1 ng,快速lamp检测体系菌体和DNA检测灵敏度分别达到了2.25cfu和2.03×10-5 ng。在特异性测试中,普通lamp检测体系与快速lamp检测体系均仅对柑橘溃疡病菌进行扩增,对非靶标菌和柑橘叶片基因组DNA不产生扩增,普通lamp与快速lamp检测体系特异性测试结果一致。快速lamp检测体系在0.5h内就可以达到普通lamp检测体系的扩增量,是普通lamp检测体系反应时间的一半,大大提高了检测的效率;快速lamp检测体系菌悬液和DNA检测灵敏度均比普通lamp检测体系提高了10 000倍。成功地建立了柑橘溃疡病菌的普通lamp及快速lamp检测方法,为柑橘溃疡病菌的检测提供了一种新的简便、快速的检测手段。

关键词：柑橘溃疡病菌环介导等温扩增 lamp 检疫