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中国人乳头瘤病毒 6型l1、l2表达及产物自动装配成病毒样颗粒(英文)

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Acta Biochimica et Biophysica Sinica 2003年第1期35卷 27-34页

作者：王淼王立良陈莲凤韩晔华邹宇宏司静懿宋国兴中国医学科学院中国协和医科大学基础医学研究所中国医学科学院中国协和医科大学基础医学研究所北京100005

为研究我国人乳头瘤病毒 6型 (HPV 6 )基因组晚期区序列变异及衣壳蛋白装配特性 ,从中国尖锐湿疣患者病损组织克隆了HPV 6l1和l2序列 ,经测序、拼接 ,获得HPV 6基因组晚期区序列。获得的两条长 2 86 9bp的序列 (GenBank登录号为AY0 15 0... 详细信息

为研究我国人乳头瘤病毒 6型 (HPV 6 )基因组晚期区序列变异及衣壳蛋白装配特性 ,从中国尖锐湿疣患者病损组织克隆了HPV 6l1和l2序列 ,经测序、拼接 ,获得HPV 6基因组晚期区序列。获得的两条长 2 86 9bp的序列 (GenBank登录号为AY0 15 0 0 6和AY0 15 0 0 8) ,属于HPV 6b亚型 ,与原型序列比较 ,在l1和l2ORF中共有 9个核苷酸变异 ,其中错义突变 4个。将l1和l2分别重组到杆状病毒 (AcNPV)表达载体 ,在Sf9细胞中探讨l1和l2的表达及装配规律。当l1和l2分别表达于Sf9细胞时 ,l1可以在细胞核中装配成病毒样颗粒 (VlP) ,而l2不能。从l1重组杆状病毒单独感染和l1、l2重组杆状病毒同时感染的细胞分别纯化VlP ,获得l1 VlP和l1+l2 VlP。二者在大小及形态上无明显差异 ,直径约 5 0nm ,与真实的病毒粒子相似。l1+l2 VlP包含摩尔比例约为 4∶1的l1和l2 ,具有HPV 6l1VlP构象表位。当用不同比例重组病毒同时感染细胞时 ,一定水平的l2表达可以使l1表达量及VlP产量提高。HPV 6基因组晚期区序列变异率 2 8% ,70 81位的A→G和 70 99位的G→A两处突变在我国HPV 6分离物中具有代表性。克隆的HPV 6l1和l2序列可以用于表达 ,表达产物 (l1或l1+l2 )可以自发装配成VlP。

关键词：人乳头瘤病毒6型 l1 l2 杆状病毒载体病毒样颗粒

The Effects of l1 Knowledge on l2(English) Reading Comprehension for Chinese Students in Middle School

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海外英语 2012年第5X期 125-127页

作者：张耿厦门大学嘉庚学院福建厦门363105

Implications of second language reading learning are often aroused by the insights of reading experience in first *** nese learners' performance concerning English reading comprehension may influenced by Chinese language or English *** pa per attempts to explore the influence of first language(Chinese) knowledge on second language(English) reading comprehension for Chi nese students in middle *** investigation concerns over three aspects of l1 knowledge:genre knowledge,reading skills,and cultur al *** firstly demonstrates the research ***,based on schema theory,it introduces the research method and partici pants in this ***,this paper presents the data of the investigation along with the data *** finally concludes that the positive effects of l1 knowledge on l2 reading comprehension overweigh its negative effects.

关键词： reading comprehension l1 Knowledge l2 Knowledge sc

A Preliminary Study of Cohesive Features in l2 Argumentative Writing by the Senior Students of a High School

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海外英语 2012年第7X期 247-249页

作者： HUANG Min-ling(Houhai Primary School,Nanshan District,Shenzhen 518000,China) Houhai Primary School Nanshan District Shenzhen 518000 China

This research made comparisons of cohesive ties between the good and poor compositions of sixty senior three students in a senior high school in Shenzhen,Guangdong Province to examine cohesive features in l2 argumentative *** findings of the present study reveals that the composition scores were significantly co-varied with the number of conjunction ties and lexical ties,as well as the total number of cohesive ties *** the three main categories of cohesive ties,lexical ties(66.7%) used most frequently in the sampled l2 ***,cohesive ties with the most frequently used,lexical ties,contribute to the quality of syntactical structure.

关键词： l2 writing SFl cohesion cohesive ties

l2 Teaching and learning in the Classroom Space

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海外英语 2014年第1X期 1-3页

作者：任锡平 Hohai University

l2 teaching and learning is a way of using language,but it happens in a particular space—the classroom space,which,to some extent,has a restriction to language *** paper provides a valuable sight into l2 teaching and learning in the classroom space,and discusses the viewpoint of how to make an actual learning of l2 under the way of teaching.

关键词： l2 teaching and learning classroom space

The Study of Factors of Interference of l1 Japanese on l2 English Pronunciation

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海外英语 2019年第12期 106-108,112页

作者：吴瑞雪 Australian National University

This article focuses on investigating whether and how the factors of AoA,exposure of l1 Japanese and motivation have an effect on the production of l2 English sound of l1 Japanese *** addition,the most effective facto... 详细信息

关键词： l1 Japanese l2 English AoA exposure motivation l1 interference l2 native-like accent

Effects of Different Reading Purposes on l2 learners' IVA

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海外英语 2015年第2期 119-120页

作者：王薇天津电子信息职业技术学院

According to the former studies, there are many factors having effects on second language(l2) learners' incidental vocabulary acquisition(IVA). This paper is aimed to reveal how reading a text with different purposes might affect IVA of l2 learners. The results of this study show that there is a closed relationship between the efficiency of IVA and reading purposes. Besides,it also has something to do with l2 learners' reading abilities.

关键词： reading purposes reading abilities l2 IVA

Exploiting lexical Properties for l2 Students of Chinese

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汉语应用语言学研究 2018年第1期 32-39页

作者： Jerome l.Packard University of Illinois at Urbana-Champaign/U.S.A.

This paper proposes lexical coding strategies that follow from experimental results demonstrating that the component of working memory known as Focal Attention (FA) is as robust in learners of Chinese as it is in native Chinese *** encoding that relies upon FA memory can be strategically employed by l2 learners of Chinese to improve the efficiency of their lexical storage and retrieval.

关键词： Chinese l2 vocabulary focal attention lexical storage and retrieval working memory

Task Repetition and the Development of l2 Oral Production

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中国语音学报 2016年第1期 62-70页

作者： ZHI Na lI Aijun JIA Yuan College of Foreign languages in Capital Normal University Institute of linguistics Chinese Academy of Social Sciences

The paper reports the developmental trajectory of Chinese EFl( English as a foreign language) learners’ oral performance in terms of their speech quantity and quality in repeated topic-based tasks. It has been a concerned issue in the theoretical and pedagogical interests that how l2 learners can enhance their oral proficiency. However,as pointed in[22],there are few studies focused particularly on the l2 speakers’ performance change,while more studies are devoted to the performance status. The current research presents a case study of six Chinese students’ English oral development addressing to the issue of learners’ dynamic change. By investigating each speaker’s variation in the speech quantity( the total amount of produced speech) and speech quality( indicated by fluency,accuracy and complexity) during the three-week intervals,we find that task repetition can positively affect the speakers’ oral production.

关键词： Task Repetition the Development l2 Oral Production

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On Migration and Illiteracy--Dealing With Texts in l2 Italian Initial-literacy Classes

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Journal of literature and Art Studies 2019年第12期9卷 1292-1298页

作者： Castrenze Nigrelli University of Palermo PalermoItaly

Acquiring the language spoken in the host country is crucial for social inclusion of *** this perspective,illiteracy represents a social problem since it has repercussions on second-language acquisition/learning *** order to investigate the role of illiteracy in l2 Italian acquisition,the paper aims at analyzing the oral productions of l2 Italian learners with equal l1 but a different level of education,i.e.,low educated *** learners’productions are collected through semi-structured video *** the perspective of the conversational analysis,the interviews are transcribed and studied taking into account also non-verbal aspects of the oral *** learners belong to the specific category of the“unaccompanied foreign minors”.These minors are characterized by a particular sociolinguistic profile as they are often plurilinguals with a low,very low,or zero level of *** lack of previous schooling limits the competence in languages(including l1)to oral competence alone,and this has repercussions on second-language acquisition and,therefore,on social *** fact,a certain competence in writing and reading has an important impact on mental *** low or zero familiarity with written texts has inevitable consequences in the second-language acquisition process,in terms of lack of meta-textual and meta-linguistic *** the comparison of the interviews,the characteristics of the learners’interlanguages show that the learners deal with the non-inflectional initial phase of the second-language acquisition process:Their linguistic varieties are certainly basic ***,data show significant differences in the learners’performance depending on(il)literacy,which is the only parameter differentiating the learners’*** particular,literacy,namely a certain,albeit low,level of education and proficiency in writing skills,has positive repercussions on the performance in terms of a greater aw

关键词： illiteracy l2 Italian plurilingualism second-language learning/teaching textual competence