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l-type calcium channel Modulates low-Intensity Pulsed Ultrasound-Induced Excitation in Cultured Hippocampal Neurons

Neuroscience Bulletin 2024年第7期40卷 921-936页

作者： Wen-Yong Fan Yi-Ming Chen Yi-Fan Wang Yu-Qi Wang Jia-Qi Hu Wen-Xu Tang Yi Feng Qian Cheng lei Xue State Key laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghai 200433China Department of Physiology and Neurobiology School of Life SciencesFudan UniversityShanghai 200438China Institute of Acoustics School of Physics Science and EngineeringTongji UniversityShanghai 200092China Key laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education Tongji UniversityShanghai 200070China Center for Rehabilitation Medicine Department of Pain ManagementZhejiang Provincial People's HospitalAffliated People's HospitalHangzhou Medical CollegeHangzhou 310014China Department of Critical Care Medicine Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 200080China Shanghai Research Institute for Intelligent Autonomous Systems Tongji UniversityShanghai 201210China Research Institute of Intelligent Complex Systems Fudan UniversityShanghai 200433China

As a noninvasive technique,ultrasound stimulation is known to modulate neuronal activity both in vitro and in *** latest explanation of this phenomenon is that the acoustic wave can activate the ion channels and further impact the electrophysiological properties of targeted ***,the underlying mechanism of low-intensity pulsed ultrasound(lIPUS)-induced neuro-modulation effects is still ***,we characterize the excitatory effects of lIPUS on spontaneous activity and the intracellular Ca^(2+)homeostasis in cultured hippocampal *** whole-cell patch clamp recording,we found that 15 min of 1-MHz lIPUS boosts the frequency of both spontaneous action potentials and spontaneous excitatory synaptic currents(sEPSCs)and also increases the amplitude of sEPSCs in hippocampal *** phenomenon lasts for>10 min after lIPUS *** with Ca^(2+)imaging,we clarified that lIPUS increases the[Ca^(2+)]cyto level by facilitating l-type Ca^(2+)channels(lTCCs).In addition,due to the[Ca^(2+)]cyto elevation by lIPUS exposure,the Ca^(2+)-dependent CaMKII-CREB pathway can be activated within 30 min to further regulate the gene transcription and protein *** work suggests that lIPUS regulates neuronal activity in a Ca^(2+)-dependent manner via *** may also explain the multi-activation effects of lIPUS beyond *** stimulation potentiates spontaneous neuronal activity by increasing Ca^(2+)influx.

关键词： low-intensity pulsed ultrasound Neuromodulation l-type calcium channel Hippocampal neuron

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High intracellular Mg2+ affects upon the activities of l-type calcium channel in guinea-pig ven-tricular myocytes

High intracellular Mg2+ affects upon the activities of L-typ...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：赵美眯雷明邵冬雪王红梅封瑞胡慧媛孙雪菲郭凤赵金生郝丽英中国医科大学药学院药物毒理教研室

This study is aimed to investigate the effects of high intracellular Mg2+（Mg2+i）upon the gating-mechanism of l-type calcium channel in guinea-pig ventricular *** adopting inside-out configuration of patch clamp technique,single channel currents of the l-type calcium channel were recorded with different Mg2+i *** control group in which Mg2+i is 0.9 mmol/l,the relative activities of calcium channel were 176.5±34.1%（n=7）.When Mg2+i concentration was increased from0.9 to 8.1 mmol/l,the relative activities of calcium channel decreased to 64.8±18.1%（n=6,P＜0.05）.Moreover,at 8.1 mmol/l Mg2+i,the mean open time of calcium channel was shortened to about 25%of that in control condition（P＜0.05）,but the mean close time of calcium channel was not *** results suggest that high Mg2+i may inhibit the channel activities,which are mainly due to the shortening of the mean open time of single l-type calcium channel.

关键词： Mg2+ l-type calcium channel ventricular myocytes patch clamp technique open-closed kinetics

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20-HETE increases NADPH oxidase-derived ROS production and stimulates l-type calcium channel via PKC-dependent mechanism in cardiomyocytes

20-HETE increases NADPH oxidase-derived ROS production and s...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Stephen T.O’Rourke Department of Pharmaceutical Sciences North Dakota State University

The production of 20-hydroxyeicosatetraenoic acid(20-HETE) is increased during ischemia-reperfusion and inhibition of 20-HETE production has been shown to reduce infarct size caused by *** study was aimed at discovering the molecular mechanism underlying the action of 20-HETE in cardiac *** effect of 20-HETE on l-type calcium channel currents(I) was examined in rat isolated cardiomyocytes by patch clamp recording in the whole-cell *** of cardiomyocytes with 20-HETE(10-100 nmol/l) resulted in a concentration-dependent increase in I and this action of 20-HETE was attenuated by a specific NADPH oxidase inhibitor,gp91ds-tat(5μmol/l),or a superoxide scavenger,PEG-SOD(25 U/ml),suggesting that NADPH-oxidase -derived superoxide is involved in the stimulatory action of 20-HETE on *** of cardiomyocytes with 20-HETE(100 nmol/l) increased both NADPH oxidase activity and superoxide production by approximately *** study the molecular mechanism mediating the 20-HETE-induced increase in NADPH oxidase activity, protein kinase C(PKC) activity was measured in *** of the cells with 20-HETE(100 nmol/l) significantly increased PKC activity,and pretreatment of cardiomyocytes with the selective PKC inhibitor,GF109203 (1μmol/l),attenuated 20-HETE-induced increases in I and in NADPH oxidase *** summary,20-HETE stimulates NADPH oxidase-derived superoxide production,which activates l-type calcium channels via a PKC-dependent mechanism in cardiomyocytes.20-HETE and 20-HETE-producing enzymes could be the novel targets for treatment of cardiac ischemic diseases.

关键词： 20-HETE l-type calcium channel PKC cardiac myocytes reactive oxygen species

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Neuropeptide W induces vasoconstriction by up-regulating the function of l-type calcium channel via NBPWR1

Neuropeptide W induces vasoconstriction by up-regulating the...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：纪丽陈红王玥王芫王觉进南京医科大学生理学系

Neuropeptide W（NPW）,an endogenous ligand for the G-protein coupled receptor NBPWR,is produced in neurons known to be important in the control of food intake and the neuroendocrine response to stress in central nerve *** the periphery,NPW is also expressed and makes some biological functions,including intracellular calcium ***,we found NPW’s receptor NBPWR1 was coexpressed with Ca V1.2 calcium channel in arterial smooth muscle,implying its roles in the Ca V1.2 of arterial smooth *** whole-cell patch clamp,NPW-23,the active form of NPW,could concentration-dependently increase the calcium currents（ICa,l）in heterolozagously transfected HEK 293 cells and isolated vascular smooth muscle *** system was used to explore the physiological function of NPW-23 in arterial constriction,we found the mesenteric arterial wall exhibited increased vascular tone when given NPW-23into the bath *** above data indicated that NPW-23 regulated ICa,l in vascular smooth muscle cells,and such a mechanism may play a role in modulating vascular constriction.

关键词： Neuropeptide W l-type calcium channel vascular tone hypertension

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Inhibitory protection of nitric oxide to l-type calcium channel in rat cardiomyocytes

Inhibitory protection of nitric oxide to L-type calcium chan...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：岳志杰余志斌徐彭涛谢满江常惠第四军医大学第四军医大学航空航天生理学教研室

Nitric oxide（NO）slightly inhibits the calcium current（ICa,l）of l-type calcium channel（lTCC）.The enhanced ICa,l induced by isoproterenol（ISO）in cardiomyocytes was decreased in n NOS knockout mice or 4-week of tail-suspended（SUS）*** underlying mechanism is not resolved ***,the purpose of the study was to investigate the effect of n NOS-derived NO on lTCC gating property of cardiomyocytes during ISO *** the present study,the expression and activity of n NOS was decreased in the myocardium of SUS *** was no difference in expression and 1C subunit with or without ISO treatmentaphosphorylation at Ser1928 of lTCC between the control（CON）and SUS *** anion radical production increased in cardiomyocytes of the SUS group after ISO stimulation.1C subunit significantly decreased in the SUS group.a S-nitrosylation of lTCC ISO-induced enhancement of ICa,l in the SUS group was less than that in the CON *** maximal ICa,l decreased to about 80%and 60%initial value at the 50th minute of ISO treatment in the CON and SUS group,*** inhibitor NAAN of n NOS reduced the maximal ICa,l to 50%initial value in the CON group,in contrast NO donor SNAP kept the maximal ICa,l in the SUS group to the similar extent of the CON group after 50 min of ISO *** steady-state activation,inactivation and recovery curves of lTCC were changed in the SUS group after ISO *** of n NOS in the CON group led similar changes with the SUS group in lTCC gating properties after ISO treatment,but SNAP in the SUS group did not restored lTCC gating properties to control level.1C subunitaIn conclusion,n NOS-derived NO-induced S-nitrosylation of lTCC slightly inhibits ICa,***,the inhibitory effect on ICa,l competitively prevents oxidation of lTCC from reactive oxygen species and protects the gating property of lTCC during long-term of ISO stimulation.

关键词： simulated weightlessness cardiomyocyte l-type calcium channel S-nitrosylation

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Amlodipine inhibits the proliferation and migration of esophageal carcinoma cells through the induction of endoplasmic reticulum stress

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World Journal of Gastroenterology 2024年第4期30卷 367-380页

作者： Yan-Min Chen Wen-Qian Yang Cheng-Wei Gu Ying-Ying Fan Yu-Zhen liu Bao-Sheng Zhao Department of Thoracic Surgery The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina Department of Oncology The Affiliated HospitalHenan Polytechnic UniversityJiaozuo 454000Henan ProvinceChina life Science Research Center The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina

BACKGROUND l-type calcium channels are the only protein channels sensitive to calcium channel blockers,and are expressed in various cancer *** Cancer Genome Atlas database shows that the mRNA levels of multiple l-type calcium channel subunits in esophageal squamous cell carcinoma tumor tissue are significantly higher than those in normal esophageal epithelial ***,we hypothesized that amlodipine,a long-acting dihydropyridine l-type calcium channel blocker,may inhibit the occurrence and development of esophageal cancer(EC).AIM To investigate the inhibitory effects of amlodipine on EC through endoplasmic reticulum(ER)*** Cav1.3 protein expression levels in 50 pairs of EC tissues and corresponding paracancerous tissues were ***,the inhibitory effects of amlodipine on proliferation and migration of EC cells in vitro were detected using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and Transwell *** vivo experiments were performed using murine xenograft *** elucidate the underlying mechanisms,in vitro cell studies were performed to confirm that ER stress plays a role in inhibition proliferation and migration of EC cells treated with *** The expression level of Cav1.3 in esophageal carcinoma was 1.6 times higher than that in paracancerous *** treatment decreased the viability of esophageal carcinoma cells in a dose-and time-dependent *** vivo animal experiments also clearly indicated that amlodipine inhibited the growth of EC tumors in ***,amlodipine reduces the migration of tumor cells by inhibiting epithelial-mesenchymal transition(EMT).Mechanistic studies have demonstrated that amlodipine induces ER stress-mediated apoptosis and suppresses ***,amlodipine-induced autophagy was characterized by an increase in autophagy lysosomes and the accumulation of light chain 3B *** combination of amlodipine with the ER stress inhibitor 4-p

关键词： l-type calcium channel Amlodipine Esophageal cancer Autophagy Endoplasmic reticulum stress

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MicroRNA-155 mediates endogenous angiotensin II type 1 receptor regulation:implications for innovative type 2 diabetes mellitus management

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World Journal of Diabetes 2023年第9期14卷 1334-1340页

作者： Konstantinos I Papadopoulos Alexandra Papadopoulou Tar-Choon Aw Department of R&D THAI StemLifeBangkok 10310Thailand Occupational and Environmental Health Services Feelgood LundLund 223-63SkåneSweden Department of laboratory Medicine Changi General HospitalSingapore 529889SingaporeSingapore Department of Medicine National University of SingaporeSingapore 119228SingaporeSingapore

type 2 diabetes mellitus(T2DM)is a lifelong condition and a threat to human *** understanding of its pathogenesis is acutely needed in order to devise innovative,preventative,and potentially curative pharmacological ***(miRNA),are small,non-coding,one-stranded RNA molecules,that can target and silence around 60%of all human genes through translational ***-155 is an ancient,evolutionarily well-conserved miRNA,with distinct expression profiles and multifunctionality,and a target repertoire of over 241 genes involved in numerous physiological and pathological processes including hematopoietic lineage differentiation,immunity,inflammation,viral infections,cancer,cardiovascular conditions,and particularly diabetes ***-155 levels are progressively reduced in aging,obesity,sarcopenia,and ***,the loss of coordinated repression of multiple miR-155 targets acting as negative regulators,such as C/EBPβ,HDAC4,and SOCS1 impacts insulin signaling,deteriorating glucose homeostasis,and causing insulin resistance(IR).Moreover,deranged regulation of the renin angiotensin aldosterone system(RAAS)through loss of Angiotensin II type 1 receptor downregulation,and negated repression of ETS-1,results in unopposed detrimental Angiotensin II effects,further promoting ***,loss of BACH1 and SOCS1 repression abolishes cytoprotective,anti-oxidant,anti-apoptotic,and anti-inflam matory cellular pathways,and promotesβ-cell *** contrast to RAAS inhibitor treatments that further decrease already reduced miR-155 levels,strategies to increase an ailing miR-155 production in T2DM,e.g.,the use of metformin,mineralocorticoid receptor blockers(spironolactone,eplerenone,finerenone),and verapamil,alone or in various combinations,represent current treatment *** the future,direct tissue delivery of miRNA analogs is likely.

关键词： Angiotensin II Angiotensin II type 1 receptor Arginase 2 l-type calcium channel Mineralocorticoid receptor MiRNA-155 Renin-angiotensin aldosterone system type 1/2 diabetes mellitus Verapamil

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Molecular mechanisms of early atrial remodeling by rapid atrial pacing in rabbits

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Journal of Medical Colleges of PlA(China) 2006年第1期21卷 13-18页

作者：马瑞彦肖颖彬钟前进陈林王学锋陈劲进 Department of Cardiovascular SurgeryXinqiao Hospital Third Military Medical UniversityChongqing 400037China

Objective: To establish a rabbit atrial fibrillation model with rapid atrial pacing (RAP) and investigate its ultrastructural changes and expressions of l-type calcium channel subunits and potassium channel Kv4. 3. Methods: Thirty-six rabbits were performed electrical stimulation through bipolar endo-cardial led by surgical technique. 600 beat per min from 0 to 48 h. Atrial ultrastructure was observed by transmission electron microscope (TEM) after different pacing times. mRNA were measured by reverse *** chain reaction (RT-PCR). Results: Atrial ultrastructure had alteration after 3 hours' pacing, such as mitochondria vacuolization, myofilament lysis and glucogen aggregation. The mRNA of the Ca2+ channel β1 and α1 subunits began to decrease after pacing of 6 h. which were paralleled with the change of Kv4. 3 mRNA. But the auxiliary subunit α2 were not affected. Conclusion: Ultrastructural changes and mRNA levels of l-type calcium channel subunits and potassium channel Kv4. 3 are decreased after RAP. with a mechanism of transcriptional down-regulation of underlying ion channels due to calcium overloading after RAP.

关键词： rapid atrial pacing ultrastructure l-type calcium channel rabbit mRNA expression

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Progress of CACNA1S Gene and Hypokalemic Periodic Paralysis

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Animal Husbandry and Feed Science 2009年第2期1卷 18-21页

作者：王雪任守文刘红林南京农业大学江苏南京210095 江苏省农业科学研究院江苏南京210014

CACNA1 S gene is the gene encoding l-type calcium channel αa-subunit. CACNA1 S gene mutations can cause hypokalemic periodic pa- ralysis （HOKPP）. The related research speculated that CACNA1 S gene was the candidate genes which affect meat quality traits. In the present ar- ticle, the biological characteristics of CACNA1 S gene, structure, genetic diseases and the research development were respectively reviewed so as to provide a reference for further research.

关键词： CACNA1 S gene l-type calcium channel α1-subunit Human hypokalemic periodic paralysis

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The effect of Ca MK II and CIP on the binding of calmodulin to cardiac Cav1.2 channel

The effect of Ca MK II and CIP on the binding of calmodulin ...

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第十一届全国钙信号和细胞功能研讨会

作者： Jingyang Su liting lyu Jianwei Mao Guigui Wei Dongxue Shao Meimi Zhao Feng Guo Huiyuan HU Xuefei Sun Guangyu Jiao Rui Feng Hao liying Department of Pharmaceutical Toxicology School of Pharmacy China Medical University Respiratory Department and Intensive Care Unit Shengjing Hospital of China Medical University

In the present study, the roles of Ca/Ca M dependent protein kinase II(Ca MKII) and Calf Intestinal Alkaline Phosphatase(CIP) on the binding of calmodulin(Ca M) to cardiac Cav1.2 channel were examined by pull-down assay method. We examined the binding of Ca M to cardiac Cav1.2 fragments after treated by Ca MKII and CIP. The experiments showed that the Cav1.2 C-terminal fragment CT1 still has the ability to bind with Ca M after treatments at different Ca concentration([Ca]) from ≈ free to 2 m M. The binding effects of Ca M to CT1 were increased by CaM KII phosphorylation with CaM-concentration dependent manner, and the binding effects of Ca M to CT1 were decreased by CIP dephosphorylation with Ca Mconcentration dependent manner. To further find the key regions in CT1 for Ca MKII and CIP regulation, we examined the binding effect of Ca M to IQ after Ca MKII and CIP treatment. The results showed that the binding effect were increased after Ca MKII treatment, and were decreased after CIP treatment in 2 mM, 10μM Ca, in additon, the effect were Ca Mconcentration dependent manner. The data imply that at higher Ca, Ca M can easily bind to CT1 after Ca MKII phosphorylation, and the binding effect was easily inhibited after CIP dephosphorylation. Furthermore, the IQ motif might be the important region for Ca MKII and CIP to mediate CaM binding.

关键词： calmodulin Ca2+/Ca M dependent protein kinase II l-type calcium channel phosphorylation

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