检索结果-南通市图书馆

Effectiveness of l-arginine/l-lysine in retarding deterioration of structural and gelling properties of duck meat myofibrillar protein during freeze-thaw cycles

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

FOOD BIOSCIENCE 2023年第1期51卷 737-744页

作者： li, Mengmeng He, Shufeng Sun, Yangying Pan, Daodong Zhou, Changyu He, Jun Ningbo Univ State Key Lab Managing Biot & Chem Threats Qual & Ningbo 315211 Peoples R China Ningbo Univ Coll Food & Pharmaceut Sci Key Lab Anim Prot Food Deep Proc Technol Zhejiang Zhejiang Malaysia Joint Res Lab Agr Prod Proc & Nu Ningbo 315800 Zhejiang Peoples R China Ningbo Univ Coll Food & Pharmaceut Sci Meishan Bonded Port Area 169 Qixing South RdMeishan Bonded Port Area Ningbo 315800 Peoples R China

This study aimed to investigate the protective effects of l-arginine (l-arg) and l-lysine (l-lys) on the structure and gel properties of myofibrillar protein (MP) in duck meat during several freeze-thaw (F -T) cycles. The analysis of the Ca2+-ATPase activity as well as total sulfhydryl and carbonyl contents demonstrated that l-arg and l-lys could slow down the oxidative denaturation of MP during F-T cycles. Circular dichroism, fluorescence spec-troscopy, and rheology measurements indicated that l-arg and l-lys improved the stability of the secondary and tertiary structure of MP and preserved its gel properties. In summary, l-arg and l-lys had the potential to pre-serve the structural stability and gel properties of MP during F-T cycles.

关键词： l-arginine l-lysine Myofibrillar protein Gelling Freeze -thaw cycle Protein structure

Competitive metabolism of l-arginine:arginase as a therapeutic target in asthma

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2011年第5期25卷 299-308页

作者： Jennifer M.Bratt Amir A.Zeki Jerold A.last Nicholas J.Kenyon Department of Internal Medicine Division of Pulmonary and Critical Care and Sleep MedicineUniversity of California

Exhaled breath nitric oxide （NO） is an accepted asthma biomarker. lung concentrations of NO and its amino acid precursor, l-arginine, are regulated by the relative expressions of the NO synthase （NOS） and arginase isoforms. Increased expression of arginase I and NOS2 occurs in murine models of allergic asthma and in biopsies of asthmatic airways. Although clinical trials involving the inhibition of NO-producing enzymes have shown mixed results, small molecule arginase inhibitors have shown potential as a therapeutic intervention in animal and cell culture models. Their transition to clinical trials is hampered by concerns regarding their safety and potential tox- icity. In this review, we discuss the paradigm of arginase and NOS competition for their substrate l-arginine in the asthmatic airway. We address the functional role of l-arginine in inflammation and the potential role of arginase inhibitors as therapeutics.

关键词： nitric oxide l-arginine arginase nor-NOHA nitrosation nitric oxide synthase

Dosage of l-arginine Preventing Acute High-dose PDD Nephrotoxicity

在线全文

学校读者我要写书评

暂无评论

The Chinese-German Journal of Clinical Oncology 2005年第6期4卷 358-360页

作者：王京芬刘秀菊郝鲁英山东省临沂市肿瘤医院内一科 276001

Objective： To explore the optimal dose of l-arginine to prevent acute high-dose （HD）-PDD nephrotoxicity. Methods： 128 cases using PDD with the dosage of 100 mg/m^2 within two days （D1, 2） in combination with l-arginine were randomly divided into 3 groups of A, B and C. The dosages of l- arginine in the 3 groups were 5 g/（m^2·d）, 10 g/（m^2·d） and 15 g/（m^2·d）, respectively. Each patient received 2 cycles chemotherapy to form self control： 1 cycle combined with l-arginine, while 1 cycle chemotherapy alone, β2-MG in urine, BUN, Cr and uric acid in blood were detected just 24 h before and after using PDD. The changes of each index in the three groups were observed in the presence or absence, and the therapeutic effects were compared among the three groups. Results： There was no significant difference in BUN, Cr and uric acid in blood before and after chemotherapy in the presence or absence, showing these indexes could not be used as markers of early acute nephrotoxicity. Urine β2-MG values in the presence and absence were 0.9120±0.6618 vs 1.5167±0.7908 （P〈0.05）, 0.5404±0.5810 vs 1.4616±0.8120 （P〈0.01）, 0.4998±0.6210 vs 1.5210±0.7710 （P〈0.01） in the groups A, B and C respectively. The excellent effective rate and total effective rate in groups A, B and C were 40.9% and 59.1%, 68.2% and 90.9%, and 77.5% and 97.5%, respectively. There was significant difference in the excellent effective rate and total effective rate between groups A and B, but not between groups B and C. Conclusion： The optimal dose of l- arginine to prevent acute HD-PDD nephrotoxicity is 10 g/（m^2·d）. Increased dosage can＇t improve the effect accordingly.

关键词： l-arginine optimal dosage PDD nephrotoxicity

Influence of l-arginine on the Expression of eNOS and COX2 in Experimental Pulmonary Thromboembolism

在线全文

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 2006年第5期26卷 524-527页

作者：张建初夏蕾张晓菊杨卫兵白明 Department of Pulmonary Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Pulmonary Medicine Affiliated Hospital of Wuhan University of Science & TechnologyWuhan 430064China

The influence of l-arginine on endothelial nitric oxide synthase （eNOS） and cyclooxygenase 2 （COX2） was observed in experimental pulmonary thromboembolism and the action mechanism on pulmonary thromboembolism was explored. Wistar rats were randomly divided into control group, model group and treatment group. Pulmonary thromboembolism models were established by auto-blood back transfusion, and l-Arg 100 mg/kg was intraperitoneally injected after successful model preparation. The animals were sacrificed at 3 h, 1 day, 3 days and 7 days after embolism. Plasma NO, TXB2 and 6-Keto-PGF1 α were detected. The expression of eNOS and COX2 protein and mRNA in pulmonary tissues was detected by immunohistochemistry and RT-PCR respectively. The results showed that pulmonary thrombosis could be seen post pulmonary embolism and inflammatory reaction was significant. Plasma NO was decreased （P〈0.01）, and the levels of TXB2, 6-Keto-PGF1α and T/P ratio were all elevated. The expression of eNOS protein and mRNA in the pulmonary tissue was down-regulated （P〈0.05）, while that of COX2 protein and mRNA was upregulated （P〈0.01）. In treatment group, the level of NO was increased, the levels of TXB2 and T/P ratio were decreased, but the level of 6-Keto-PGF1 α was increased. The expression of eNOS protein and mRNA in pulmonary tissue was upregulated （P〈0.05）, while that of COX2 protein and mRNA was down-regulated （P〈0.05）. In conclusion, l-arginine can educe the role of pulmonary tissue protection through up-regulating the expression of intra-pulmonary NOS and down -regulating COX2 in pulmonary thromboembolism.

关键词： pulmonary thromboembolism l-arginine nitric oxide synthase cyclooxygenase2

l—arginine及增龄对大鼠阴茎组织中NO—cGMP通路的影响研究

在线全文

学校读者我要写书评

暂无评论

中华临床医药杂志（北京） 2002年第18期3卷 14-17页

作者：吴晓军金锡御等重庆西南医院全军泌尿外科专科中心400038

目的：探讨衰老过程中左旋精氨酸（l-arginine)对大鼠阴茎组织中“NO-cGMP通路”的影响及意义。方法：将不同月龄（2，8，16，24月）大鼠随机分为实验组与治疗组（喂养l-arginine)。进行了以下研究；（1）阴茎组织中一氧化氮（nitricoxi... 详细信息

目的：探讨衰老过程中左旋精氨酸（l-arginine)对大鼠阴茎组织中“NO-cGMP通路”的影响及意义。方法：将不同月龄（2，8，16，24月）大鼠随机分为实验组与治疗组（喂养l-arginine)。进行了以下研究；（1）阴茎组织中一氧化氮（nitricoxide,NO),环磷酸鸟苷（cGmp)含量测定；（2）阴茎组织一氧化氮合酶（Nitric Oxide Synthase,NOS0活性及NADPH组化变化。结果（随龄增加）：（1）阴茎组织中NO含量先升高后降低，8月龄最高，24月龄最低，NOS活性变化与其一致，各月龄组间差别均非常显著（Pl-arginine长时间喂养大鼠后，阴茎组织中NOS活性及NO，cGMP含量均显著增加（Pl-arginine增强NOS活性，增加NO，cGMP含量有明显作用。表明l-arginine有用于治疗ED的潜在临床价值。

关键词：阴茎勃起增龄一氧化氮 cGMP l-arginine 左旋精氨酸环磷酸鸟苷

l-arginine在高原鼠肝脏缺血再灌注损伤时肠道细菌易位中的作用研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

医学理论与实践 2002年第7期15卷 745-746页

作者：童宗焰李素芝王茂旭刘厚东郑建伟周萍西藏军区总医院普通外科拉萨850003

目的:探讨肝脏缺血再灌注损伤时肠道细菌易位的机制及精氨酸能否有效改善肠道细菌易位。方法:在大鼠肝脏缺血再灌注损伤模型基础上,对门静脉血、回肠系膜淋巴结进行肠道细菌培养。结果:肝脏缺血再灌注损伤时,血液及淋巴结可能培养出大... 详细信息

目的:探讨肝脏缺血再灌注损伤时肠道细菌易位的机制及精氨酸能否有效改善肠道细菌易位。方法:在大鼠肝脏缺血再灌注损伤模型基础上,对门静脉血、回肠系膜淋巴结进行肠道细菌培养。结果:肝脏缺血再灌注损伤时,血液及淋巴结可能培养出大肠埃希氏菌。但精氨酸预处理组与相同阻断时间组之间无差异。结论:高原地区第一肝门阻断的时间对肠道细菌易位有显著影响,但精氨酸对肝缺血再灌注损伤的肠道细菌易位无明显的保护作用。

关键词：肝脏缺血再灌注损伤肠道细菌易位 l-arginine 高原

The impact of estrogen and l-arginine on serum NO and eNOS expression in aorta of aged rats

在线全文

学校读者我要写书评

暂无评论

South China Journal of Cardiology 2012年第1期13卷 13-18,28页

作者：赵频祁秀娟吕映频景秀菊刘相萍 Affiliated Hospital of Qingdao University Medical College

Background Cardiovascular diseases （CVD） are less prevalent in postmenopausal women received estrogen replacement therapy （ERT） than those who did not receive *** study has shown that the increase of nitric oxide （NO） synthesis is one of the cardioprotective effects of *** study investigated the effects of estrogen and l-arginine （l-Arg） on serum NO concentrations and the possible regulatory role in endothelial nitric oxide synthase （eNOS） expression in aortas of aged *** Fifty aged female wistar rats （18-20 months） were randomly divided into five groups （n=10）：Sham group （sham operated,0.9 % NaCl 10 μg every three day for 4 months）,OVX group （ovariectomized,0.9 % NaCl 10 μg i.m every three day for 4 months）,OVE group （ovariectomized ＋ 17β-estradiol 10 μg i.m every three day for 4 months）,OVE ＋ l-Arg group （ovariectomized ＋ 17β-estradiol 10ug i.m every three day ＋ 2.25 % l-Arg contained in drinking water every day for 4 months） and l-Arg group （ovariectomized ＋ 2.25 % l-Arg contained in drinking water every day for 4 months）.NO concentration and the expression of eNOS mRNA in aorta were measured after 4 *** Serum NO synthesis did not alter after ovariectomized （P=0.362）,but were increased in OVE group,l-Arg group and OVE ＋ l-Arg group compared with OVX group （P 0.05,P 0.05,P 0.01,respectively）.NO concentration also increased in OVE ＋ l-Arg group when compared with OVE group （P 0.05） or l-Arg group （P 0.05）.There was no significant difference in eNOS mRNA expression in aortas of aged rats between sham,OVX,OVE,OVE ＋ l-Arg and l-Arg group （F=0.550,P=0.700）.Conclusions Estrogen treatment and l-Arg supplementation increase serum NO synthesis,but do not upregulate eNOS mRNA expression in aortas of aged rats.

关键词： nitric oxide endothelial nitric oxide synthase l-arginine estrogen

BQ123与l-arginine在肝脏缺血再灌注损伤中的作用研究

在线全文

学校读者我要写书评

暂无评论

肝胆外科杂志 2001年第6期9卷 472-473页

作者：姜永生叶启发陈卫民郭晖朱彤姜汉英夏穗生华中科技大学同济医学院附属同济医院器官移植研究所武汉430030

目的　探讨肝脏缺血再灌注损伤的机制及 BQ12 3或 (和 ) l -arginine能否有效改善肝脏缺血再灌注损伤。方法　在大鼠肝脏缺血再灌注损伤模型基础上 ,对组织形态学、肝脏酶学、透明质酸、血浆内皮素及免疫组织化学染色情况进行观测。结... 详细信息

目的　探讨肝脏缺血再灌注损伤的机制及 BQ12 3或 (和 ) l -arginine能否有效改善肝脏缺血再灌注损伤。方法　在大鼠肝脏缺血再灌注损伤模型基础上 ,对组织形态学、肝脏酶学、透明质酸、血浆内皮素及免疫组织化学染色情况进行观测。结果　肝脏缺血再灌注损伤时 ,血肝酶、透明质酶、血浆内皮素水平均显著增高 ,再灌注前使用 BQ12 3或 (和 ) l-arginine的肝脏 ,其组织结构及功能损伤均显著减轻。结论　肝脏缺血再灌注损伤与肝脏微循环改善有关 ,若能在肝脏再灌注前使用BQ12 3或 (和 ) l -arginine,可有效改善肝脏微循环。

关键词：肝脏缺血再灌注损伤微循环 BQ123 l-arginine

l-arginine对蛛网膜下腔出血大鼠脑组织水电解质含量的影响

在线全文

学校读者我要写书评

暂无评论

中国微循环 2002年第5期6卷 303-303页

作者：杨明峰孙保亮夏作理朱良珍邱平明泰山医学院脑微循环研究所山东泰安271000

目的探讨一氧化氮(nitricoxide,NO)合成底物l -arginine(l -Arg)对蛛网膜下腔出血 (subarachnoidhemorrhage,SAH)后继发性脑损害的保护作用。方法采用血管内穿刺法刺破脑底动脉环建立大鼠SAH模型 ,将动物随机分为SAH组和SAH +l -Arg组。... 详细信息

目的探讨一氧化氮(nitricoxide,NO)合成底物l -arginine(l -Arg)对蛛网膜下腔出血 (subarachnoidhemorrhage,SAH)后继发性脑损害的保护作用。方法采用血管内穿刺法刺破脑底动脉环建立大鼠SAH模型 ,将动物随机分为SAH组和SAH +l -Arg组。l -Arg(Sigma)于术前30min按0.5g/kg腹腔注射 ,每隔6h以同样的剂量追加1次。在立体定向仪控制下 ,用激光多普勒血流计检测24h内大脑顶叶皮层局部脑血流量 (regionalcerebralbloodflow,rCBF) ;将动物在不同时间点处死后用干湿重比较法测定脑组织含水量 ,原子吸收分光光度计测量不同时间点脑组织Na +、K +和Ca2 +含量。股动脉插管监测血压和血气。结果SAH组术后rCBF迅速降低 ,1h达最低值 ,24h内无明显恢复 ;SAH后6h、24h脑组织含水率和Na +含量明显增加 ,K +含量减低 ;脑组织Ca2 +含量在SAH后1h开始显著增加,并持续24h。SAH +l -Arg组rCBF下降的速度减慢 ,下降的程度减轻 ;脑组织水、Na +含量增加的程度减轻 ,K +丢失减少 ,同时脑组织Ca2 +积聚减轻。结论NO合成底物l

关键词：蛛网膜下腔出血大鼠脑组织水电解质含量 l-arginine 脑保护作用