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The toxicity of hydroxylated and carboxylated multi-walled carbon nanotubes to human endothelial cells was not exacerbated by ER stress inducer

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Chinese Chemical Letters 2019年第3期30卷 582-586页

作者： Zhen Li Ting Liu Jimin Long Yang Wu Biao Yan Ping Ma Yi Cao Laboratory of Environment-immunological and Neurological Diseases School of Basic Medical Sciences Hubei University of Science and Technology Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education Laboratory of Biochemistry College of Chemistry Xiangtan University

Hydroxylated multi-walled carbon nanotubes(h-MWCNTs) and carboxylated MWCNTs(c-MWCNTs)have potential applications in biomedicine, but their toxicity to human endothelial cells under stressed conditions associated with chronic diseases was less studied in vitro. This study stressed human umbilical vein endothelial cells(HUVECs) with ER stress inducer thapsigargin(TG), and investigated the toxicity of h-MWCNTs and c-MWCNTs to normal and stressed HUVECs. h-MWCNTs and c-MWCNTs modestly reduced cellular viability, significantly promoted soluble ICAM-1(sICAM-1),soluble VCAM-1(sVCAM-1) as well as intracellular ROS, and decreased the expression of transcription factor KLF2 and KLF4. Pre-treatment with TG significantly reduced cellular viability, promoted IL-6 and THP-1 monocyte adhesion, and increased the expression of a panel of ER stress genes. ANOVA indicated no interaction between MWCNTs and TG pre-treatment on most of the endpoints. It was concluded that the toxicity of h-MWCNTs and c-MWCNTs to HUVECs might not be exacerbated by ER stress

关键词： Multi-walled carbon nanotubes (MWCNTs) Human umbilical vein endothelial cells (HUVECs) ER stress kruppel-like factor (KLF) Toxicity

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KLF8在缺氧诱导胃癌细胞多药耐药中的作用

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现代肿瘤医学 2016年第5期24卷 693-698页

作者：杨雪王亚芳张慧刘理礼第四军医大学附属唐都医院肿瘤科陕西西安710038

目的:探讨KLF8(kruppel-like factor 8)在缺氧诱导胃癌细胞多药耐药表型中的作用。方法:利用Western blot和实时定量PCR的方法检测三株胃癌细胞系MKN28、MKN45、SGC7901在常氧及缺氧条件下KLF8的表达水平。Western blot和实时定量PCR检... 详细信息

目的:探讨KLF8(kruppel-like factor 8)在缺氧诱导胃癌细胞多药耐药表型中的作用。方法:利用Western blot和实时定量PCR的方法检测三株胃癌细胞系MKN28、MKN45、SGC7901在常氧及缺氧条件下KLF8的表达水平。Western blot和实时定量PCR检测胃癌耐药细胞系SGC7901/VCR、SGC7901/ADR和亲本细胞系SGC7901中KLF8的表达水平。构建KLF8正义表达载体及KLF8小干扰RNA的慢病毒载体,将其转染入实验细胞中,用Western blot和实时定量PCR的方法检测KLF8表达量的变化。MTT、Annexin V/PI染色法及阿霉素蓄积量与潴留实验检测KLF8在缺氧诱导胃癌细胞多药耐药表型中的作用。结果:缺氧可诱导胃癌细胞系MKN28、MKN45、SGC7901中KLF8的表达升高。与胃癌亲本细胞系相比,KLF8在胃癌耐药细胞系中的表达升高,并且在SGC7901/VCR细胞中表达升高的更为明显。外源性转染KLF8后可显著增加胃癌细胞中KLF8的表达量,KLF8小干扰RNA可有效降低常氧及缺氧条件下胃癌细胞中KLF8的表达。常氧条件下,外源性转染KLF8的正义表达载体可增加胃癌细胞对不同化疗药物的耐受性,降低化疗药物诱导的胃癌细胞凋亡指数,同时可增加细胞内阿霉素的泵出率。缺氧条件下,KLF8小干扰RNA能够逆转胃癌细胞中上述现象的发生。结论:初步实验结果发现了一个新的缺氧反应分子-KLF8。表型试验证实该分子在缺氧诱导胃癌多药耐药中发挥作用。

关键词： kruppel-like factor 8 多药耐药胃癌缺氧

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KLF2调节内皮细胞功能的研究进展

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中华临床医师杂志（电子版） 2014年第9期8卷 108-112页

作者：刘铸容皮光环川北医学院附属医院儿科四川南充637000

Krüppel样转录因子2(KLF2)是Krüppel转录因子家族的成员之一,参与细胞分化和组织发育。最近研究表明KLF2在调节血管生理功能中起重要作用,本文主要概括KLF2对血管内皮细胞生物活性的调节。

关键词： kruppel样转录因子2 血管内皮细胞转录调节 kruppel-like factor 2

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Correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration

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Journal of Hainan Medical University 2017年第23期23卷 80-83页

作者： Tao Zhang Hui-Lin Mao Li Feng Department of Pediatric Surgery the Central Hospital of Enshi Autonomous Prefecture Hubei ProvinceEnshiHubei Province445000

Objective: To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration. Methods: A total of 56 children who were diagnosed with neuroblastoma in the Central Hospital of Enshi Autonomous Prefecture between May 2014 and February 2017 were selected as the NB group of the study, and the lesion tissue was collected;38 children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to serious hydronephrosis during the same period were selected as the control group of the study, and the normal adrenal gland tissue was collected. The mRNA expression and protein expression of KLF4 and UBE2C as well as the protein expression of cell adhesion molecules and migration molecules in clinical tissue samples were determined. Results: The mRNA expression and protein expression of KLF4 in neuroblastoma tissue of NB group were greatly lower than those of control group whereas the mRNA expression and protein expression of UBE2C were greatly higher than those of control group;PDLIM1, AMF, GPx1, L1CAM, Nrg1, RANK, RANKL, Inβ1, MTA1 and MMP9 protein expression in neuroblastoma tissue of NB group were greatly higher than those of control group, negatively correlated with the protein expression KLF4, and positively correlated with the protein expression of UBE2C. Conclusion: The low expression of KLF4 and the high expression of UBE2C in neuroblastoma can promote the adhesion and migration of tumor cells.

关键词： Neuroblastoma kruppel-like factor 4 Ubiquitin-conjugating enzyme 2C Adhesion Migration

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