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Expression Profiles of psbA, ALS, EPSPS, and Other Chloroplastic Genes in Response to PSII-, ALS-, and EPSPS-Inhibitor Treatments in kochia scoparia

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American Journal of Plant Sciences 2017年第3期8卷 451-470页

作者： Vijay K. Varanasi Shahniyar Bayramov P. V. Vara Prasad Mithila Jugulam Department of Agronomy Kansas State University Manhattan USA Institute of Botany Azerbaijan National Academy of Sciences Baku Azerbaijan

kochia (kochia scoparia L. Schrad.), also known as tumbleweed, is an economically important annual C4 broadleaf weed found throughout the US Great Plains. Several herbicides with different modes of action are used in the management of kochia. The effect of commonly used herbicides on the expression of their target site(s) and photosynthetic/chloroplastic genes is poorly understood in weed species, including kochia. The objective of this research was to characterize the expression profiles of herbicide target-site genes, KspsbA, KsALS, and KsEPSPS upon treatment with PSII- (e.g. atrazine), ALS- (e.g. chlorsulfuron), and EPSPS- (e.g. glyphosate)-inhibitors, respectively, in kochia. Furthermore, the expression of genes involved in photosynthesis (e.g. KsRubisco, KsCAB, and KsPPDK) was also determined in response to these herbicide treatments. KspsbA was strongly upregulated (>200-fold) 24 h after atrazine treatment. Transcript levels of the KsALS or KsEPSPS genes were 7 and 3-fold higher 24 h after chlorsulfuron or glyphosate treatment, respectively. KsRubisco, a Calvin cycle gene important for CO2 fixation, was upregulated 7 and 2.6-fold 8 and 24 h after glyphosate and chlorsulfuron treatments, whereas it downregulated 8 and 24 h after atrazine treatment. The transcript levels of KsPPDK remained unchanged after glyphosate treatment but increased 1.8-fold and decreased 2-fold at 24 h after chlorsulfuron and atrazine treatments, respectively. KsCAB remained unchanged after chlorsulfuron treatment, but was downregulated after glyphosate and atrazine treatments. The results show that herbicide treatments not only affect the respective target-site gene expression, but also influence the genes involved in the critical photosynthetic pathway.

关键词： kochia scoparia L. Schrad. C4 Herbicide Stress Gene Expression psbA ALS EPSPS Photosynthesis Rubisco PPDK CAB

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In vitro antitumor efficacy of kochia indica extract on human hepatocellular carcinoma cell line with or without 5-fluorouracil

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Hepatoma Research 2017年第7期3卷 149-155页

作者： Nabil Mohie Abdel-Hamid Ghada A.Tabl Yousry EEl-Bolkiny Walaa O.Zeina Department of Biochemistry Faculty of PharmacyKafrelsheikh UniversityKafrelsheikh33516Egypt Department of Zoology Faculty of ScienceTanta UniversityTanta71561Egypt.

Aim: Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and the sixth most common cancer worldwide. The resistance to chemotherapy is a major obstacle in the treatment of HCC, necessitating the discovery of additional agents. There is a growing use of anticancer complementary and alternative medicine worldwide. Therefore, the aim of present study was focused on the confirmation of the suitability and validity of the new markers which would be achieved by demonstrating their significant change and reproducible expression during disease and disease management. Methods: HepG2 cell line was used to provide a source for HCC cells. The cell cultures were divided into 4 groups: control untreated group, 5-fluorouracil (5-FU) treated group as a standard chemotherapy for HCC (positive control) with the following doses (15.625, 31.2, 62.5, 125, 250 μg/mL), kochia indica extract treated group with the following concentration (12.5, 25, 50, 100, 200 μg/mL) and the group treated with a combination of 5-FU and kochia indica in different ratios. Results: Treatment with kochia indica extract, 5-FU and the combined treatment showed a significant cytotoxicity to HepG2 cells, with different IC50 values, when compared to the control. Regarding toxic effect, 5-FU showed IC50 = 237.56 μg/mL which is lower cytotoxic in compared to kochia indica with IC50 =120.5 μg/mL. The results also revealed that tumor cells were more resistant to 5-FU. Alternatively, the co-treatment with kochia indica extract ameliorated the toxicity induced by 5-FU and enhanced its therapeutic potency, either by synergistic effect of both agents and/or due to its flavonoid components that may enhance the physiological properties of the cell membranes, facilitating 5-FU entrance into tumor cells. This decreased its therapeutic dose to less than 250 μg/mL by combination therapy. Conclusion: Present findings assume that kochia indica extract co-therapy can ameliorate the side effects of 5-FU on He

关键词： Hepatocellular carcinoma kochia indica 5-fluorouracil cells of hepatoma cell line chemosensitization

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Weed Control and Crop Safety with Premixed S-Metolachlor and Sulfentrazone in Sunflower

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American Journal of Plant Sciences 2012年第11期3卷 1625-1631页

作者： Seshadri S. Reddy Phillip W. Stahlman Patrick W. Geier Curtis R. Thompson 2014 Throckmorton Hall Kansas State University Manhattan USA Agricultural Research Center Kansas State University Hays USA

A preliminary study conducted in the central USA near Colby and Hays, Kansas (KS) in 2010 indicated a premix of S-metolachlor & sulfentrazone codenamed F7583 (Broadaxe?) had good potential for use in sunflower (Helianthus annuus L.). Additional studies were conducted in 2011 at Colby, Hays, Manhattan, KS to refine rate and application timing of F7583 for weed control and crop safety. Four rates of F7583 (860, 1100, 1350 and 1840 g·ha-1) were compared to single rates of S-metolachlor and pendimethalin, and applied 21 days preplant versus preemergence (PRE). F7583 at ≥1100 g·ha-1 applied preplant or PRE controlled Palmer amaranth (Amaranthus palmeri S. Wats.) and kochia [kochia scoparia (L.) Schrad.] ≥95% and 100%, respectively in neutral pH soils. In slightly acidic soils, PRE application of F7583 was more effective against Palmer amaranth and grass weeds compared to preplant application. No benefit was gained by increasing the rate of F7583 from 1100 to 1350 g·ha-1 at either application timing. Puncturevine (Tribulus terrestris L.) control was not commercially satisfactory with F7583 at any rate or time of application. Both S-metolachlor at 1070 g·ha-1 and pendimethalin at 1600 g·ha-1 applied either preplant or PRE were considerably less effective on all three broadleaf weeds compared to F7583 treatments. Individually, S-metolachlor and pendimethalin were more effective when applied PRE compared to preplant application. F7583 did not reduce sunflower plant population or visibly injure sunflower anytime during the season.

关键词： Broadaxe Sulfentrazone S-Metolachlor Sunflower Crop Injury kochia Palmer Amaranth

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Broadleaf Weed Control in Sunflower (Helianthus annuus) with Preemergence-Applied Pyroxasulfone with and without Sulfentrazone

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Agricultural Sciences 2015年第11期6卷 1309-1316页

作者： Seshadri S. Reddy Phillip W. Stahlman Patrick W. Geier Agricultural Research Center Kansas State University Hays KS USA

A field study was conducted at two locations in Kansas, USA in 2011 and 2012 to test weed control efficacy and crop response to preemergence-applied pyroxasulfone alone and in combination with sulfentrazone in sunflower. Treatments included three rates of pyroxasulfone (100, 200 and 400 g·ha-1) applied alone and tank-mixed with sulfentrazone at 70, 140 and 280 g·ha-1. Commercial standards sulfentrazone at 140 g·ha-1 + pendimethalin at 1390 g·ha-1 and sulfentrazone at 140 g·ha-1 + S-metolachlor at 1280 g·ha-1 were also included. Pyroxasulfone at 100 g·ha-1 controlled Palmer amaranth 87% at 3 weeks after application (WAA), but control decreased to 76% at 6 WAA. Increasing pyroxasulfone rate to ≥200 g·ha-1 or tank mixing with sulfentazone at 140 g·ha-1 provided ≥90% Palmer amaranth control for at least 6 WAA. Sulfentrazone alone at 70 g·ha-1 controlled Palmer amaranth 77% at 3 WAA, but control dropped to 69% at 6 WAA. Increasing sulfentrazone rate from 70 to 140 or 280 g·ha-1 increased control to >90% at 3 WAA, but did not maintain acceptable control at 6 WAA. Tank mixing sulfentrazone at 140 g·ha-1 with pendimethalin at 1390 g·ha-1 or S-metolachlor at 1280 g·ha-1 controlled Palmer amaranth ≥90 and 84% at 3 WAA and 6 WAA, respectively. The lowest rate of pyroxasulfone (100 g·ha-1) controlled kochia 98% and the control was complete with all other treatments. However, no treatment provided as much as 90% puncturevine control at 3 WAA and the control was commercially unacceptable (<75%) at 6 WAA. No treatment visibly injured sunflower anytime during the season or reduced sunflower plant population.

关键词： Pyroxasulfone Sulfentrazone Sunflower Weed Control Palmer Amaranth kochia

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Evaluation of Preemergence Herbicides for Crop Safety and Weed Control in Safflower

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American Journal of Plant Sciences 2017年第10期8卷 2358-2366页

作者： Prashant Jha Vipan Kumar Charlemagne A. Lim Ramawatar Yadav Southern Agricultural Research Center Department of Research Centers Montana State University Bozeman MT USA

Weed management in safflower (Carthamus tinctorious L.) is a major challenge for growers due to very limited herbicide options available, particularly for broadleaf weed control. Field experiments were conducted at the Montana State University Southern Agricultural Research Center (MSU-SARC) near Huntley, MT in 2015 and 2016 to evaluate preemergence (PRE) soil-residual herbicides for crop safety and season-long broadleaf weed control in safflower. Among all herbicide programs tested, only sulfentrazone (105 g·ai·ha-1) alone or with pendimethalin (1064 g·ai·ha-1) caused 4% to 12% early-season visible injury to safflower, although the injury was not evident beyond 30 DAT. Sulfentrazone alone or with pendimethalin and pyroxasulfone (59 g·ai·ha-1) with pendimethalin had a season-long residual activity on kochia [kochia scoparia (L.) Schrad] and Russian-thistle (Salsola tragus L), with 89% to 99% control at 60 DAT, and up to 98% reduction in weed density compared with dimethenamid-P (213 g·ai·ha-1) and S-metolachlor (433 g·ai·ha-1) at 65 DAT. Pyroxasulfone (59 or 118 g·ai·ha-1) alone or dimethenamid-P with pendimethalin provided a moderate to good control (65% to 79% at 60 DAT) of kochia and Russian-thistle. However, the end-season control of kochia or Russian-thistle was inadequate ( S-metolachlor alone program. Safflower grain yield with sulfentrazone alone or with pendimethalin, pyroxasulfone alone or with pendimethalin, and dimethenamid-P with pendimethalin averaged 3559 kg·ha-1, which was 195% higher compared with the nontreated check. In conclusion, sulfentrazone and pyroxasulfone or dimethenamid-P in combination with pendimethalin will be effective PRE herbicide programs for kochia and Russian-thistle control in safflower.

关键词： Safflower Weed Control Preemergence Herbicide kochia Russian-Thistle

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