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国外医学参考资料.药学分册 1975年第3期 192-192页

作者：叶清泉

标准的 kirby-bauer 纸片法作药敏试验是临床应用抗菌素的可靠依据。但此法费时较长(18～20小时),影响临床用药。作者改用四唑类染料喷雾,可于较短时间内使生长区和抑菌圈之间的界限更加清晰,因而成为一种快速的方法。文中并报导了用临... 详细信息

标准的 kirby-bauer 纸片法作药敏试验是临床应用抗菌素的可靠依据。但此法费时较长(18～20小时),影响临床用药。作者改用四唑类染料喷雾,可于较短时间内使生长区和抑菌圈之间的界限更加清晰,因而成为一种快速的方法。文中并报导了用临床分离的大肠杆菌和粘质赛氏杆菌将原方法与改进

关键词：药敏试验抗菌素 kirby-bauer

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In-vitro antimicrobial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 787-792页

作者： Sathish Kumar SR Kokati Venkata Bhaskara Rao Molecular and Microbiology Research Laboratory Environmental Biotechnology DivisionSchool of Bio Sciences and TechnologyVIT University

Objective:To investigate the antibacterial aclivily of marine actinobacteria against multidrug resistance Staphylococcus aureus(MDRSA).Methods:Fifty one actinobacterial strains were isolated from salt pans soil,costal area in Kothapattanam,Ongole,Andhra *** screening was done using cross-streak method against *** bioaclive compounds are extracted from efficient actinobacteria using solvent *** antimicrobial activity of crude and solvent extracts was perfomied using kirby-bauer *** for ethyl acetate extract was determined by modified agar well diffusion *** potent actinobacteria are identified using Nonomura key,Shirling and Gottlieb 1966 with Bergey's manual of determinative ***:Among the fifty one isolates screened for antibacterial activity,SRB25were found efficient against *** ethyl acetate extracts showed high inhibition against test *** test was performed with the ethyl acetate extract against MDRSA and found to be 1 000μg/*** isolaled actinobacteria are identified as Streptomyces sp with the help of Nonomura ***:The current investigation reveals that the marine actinobacteria from salt pan environment can be able to produce new drug molecules against drug resistant microorganisms.

关键词： Marine actinobacteria Salt pan Multi drug resistance Staphylococcus aureus Cross streak method kirby-bauer method MIC Nonomura key

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Antimicrobial potential of Actinomycetes species isolated from marine environment

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Asian Pacific Journal of Tropical Biomedicine 2012年第6期2卷 469-473页

作者： Valli S Suvathi Sugasini S Aysha OS Nirmala P Vinoth Kumar P Reena A PG and Research Department of Microbiology Mohamed Sathak College of Arts & Science

Objective:To evaluate the antimicrobial activity of Actinomycetes species isolated from marine ***:Twenty one strains of Actinomyceteswere isolated from samples of Royapuram, Muttukadu, Mahabalipuram sea shores and Adyar estuary. Preliminary screening was done using cross-streak method against two gram-positive and eight gram-negative *** most potent strains C11 and C12 were selected from which antibacterial substances were extracted. The antibacterial activities of the extracts were performed using kirby-bauer disc diffusion method. Molecular identification of those isolates was ***:All those twenty one isolates were active against at least one of the test organisms. Morphological characters were recorded. C11 showed activity againstStaphylococcusspecies(13.0暲0.5 mm),Vibrio harveyi(11.0暲0.2 mm),Pseudomonas species(12.0暲0.3 mm). C12 showed activity against Staphylococcus species(16.0暲0.4 mm),Bacillus subtilis(11.0暲0.2 mm),Vibrio harveyi(9.0暲0.1 mm),Pseudomonas species(10.0暲0.2 mm). 16S rRNA pattern strongly suggested that C11 and C12 strains were Streptomyces ***:The results of the present investigation reveal that the marineActinomycetesfrom coastal environment are the potent source of novel ***, characterization and study of Actinom ycetescan be useful in discovery of novel species of Actinomy cetes.

关键词： MarineActinomycetes Antimicrobial activity Streptomyces 16S rRNA Cross-streak method Antibacterial substance kirby-bauer disc diffusion method Molecular identification Morphological character

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The synergistic effects of green tea polyphenols and antibiotics against potential pathogens

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Advances in Bioscience and Biotechnology 2013年第11期4卷 959-967页

作者： Bobak Haghjoo Lee H. Lee Umme Habiba Hassan Tahir Moe Olabi Tin-Chun Chu Department of Biological Sciences Seton Hall University South Orange USA Department of Biology & Molecular Biology Montclair State University Montclair USA

Green tea leaves contain many polyphenolic compounds such as (-)-epicatechin (EC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin (EGC), and (-)epigallocatechin-3-gallate (EGCG). These polyphenol compounds have been implicated to have distinct properties that combat the harmful effects of cell proliferation. They contain certain anti-viral and antibacterial properties that inhibit growth. In this study, 1% green tea and modified lipophilic green tea polyphenols (GTP and LTP) were used in combination with the most commonly prescribed antibiotics to study their effects on gram-positive, gram-negative, and acid-fast bacteria. The results indicated that 1% GTP and 1% LTP provided different synergistic effects on several antibiotics in various bacteria. It was found that 1% GTP works the best synergistically against Enterobacter aerogenes, making the resistant strain susceptible to 8 out of 12 antibiotics used. 1% LTP worked the best on Escherichia coli and was able to convert 7 antibiotic resistant categories to susceptible. In addition, 1% LTP was also able to inhibit the growth of Serratia marcescens synergistically with 3 antibiotics. These results suggest that 1% GTP and 1% LTP provide beneficial effects on selected antibiotics against microbial growth and are able to reverse the antibiotic resistance to susceptible. Green tea polyphenols could serve as natural alternatives to combat against antibiotic resistance pathogens.

关键词： GTP LTP Green Tea Polyphenols Pathogenic Microorganisms kirby-bauer Disk Diffusion Method Antibiotic Resistance

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