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mutations of voltage-gated sodium channel contribute to pyrethroid resistance in Panonychus citri

Insect Science 2024年第3期31卷 803-816页

作者： Deng Pan Qiu-Juan Luo Andrias O.O'Reilly Guo-Rui Yuan Jin-Jun Wang Wei Dou Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionAcademy of Agricultural SciencesSouthwest UniversityChongqingChina Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Academy of Agricultural SciencesSouthwest UniversityChongqingChina School of Biological and Environmental Sciences Liverpool John Moores UniversityLiverpoolUK

Insecticide resistance in Panonychus citri is a major obstacle to mite control in citrus *** insecticides are continually used to control mites in China,although resistance to pyrethroids has evolved in some ***,the resistance to the pyrethroid fenpropathrin was investigated and 7 out of 8 field-collected populations of P citri exhibited a high level of resistance,ranging from 171-fold to 15391-fold higher than the susceptible(SS)comparison *** voltage-gated sodium channel(VGSC)mutations were identified in the tested populations:L1031V,F1747L,and *** sequencing was used to evaluate the frequency of these mutations in the 19 field populations.L1031V and F1751I were present in all populations at frequencies of 11.6%-82.1%and 0.5%-31.8%,respectively,whereas the F1747L mutation was only present in 12 populations from Chongqing,Sichuan,Guangxi,and Yunnan *** of these mutations singly or in combination into transgenic flies significantly increased their resistance to fenpropathrin and these flies also exhibited reduced mortality after exposure to the pyrethroids permethrin andβ-*** citri VGSC homology modeling and ligand docking indicate that F1747 and F1751 form direct binding contacts with pyrethroids,which are lost with mutation,whereas L1031 mutation may diminish pyrethroid effects through an allosteric ***,the results provide molecular markers for monitoring pest resistance to pyrethroids and offer new insights into the basis of pyrethroid actions on sodium channels.

关键词： amplicon sequencing kdr mutation molecular docking Panonychus citri pyrethroid resistance transgenic flies

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First detection of F1534C knockdown resistance mutation in Aedes aegypti(Diptera:Culicidae)from Cameroon

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Infectious Diseases of Poverty 2020年第6期9卷 51-62页

作者： Aurelie P.Yougang Basile Kamgang Theodel A.Wilson Bahun Armel N.Tedjou Daniel Nguiffo-Nguete Flobert Njiokou Charles S.Wondji Centre for Research in Infectious Diseases P.O.Box 13591YaoundéCameroon Parasitology and Ecology Laboratory Department of Animal Biology and PhysiologyFaculty of ScienceUniversity of Yaoundé1P.O.Box 812YaoundéCameroon Laboratory of Vertebrate and Invertebrate Bioecology Faculty of Science and TechnologyMarien-Ngouabi UniversityBrazzavilleCongo Laboratory of Biology and Applied Ecology Department of Animal BiologyFaculty of ScienceUniversity of DschangP.O.Box 96DschangCameroon Liverpool School of Tropical Medicine Pembroke placeLiverpoolL35QAUK

Background: Aedes borne viral diseases,notably dengue,are increasingly reported in Cameroon with Aedes aegypti being a major *** on insecticide resistance of this vector and underlying mechanisms needed for outbreak preparedness remain scarce in ***,we present the nationwide distribution of insecticide resistance in *** and investigate the potential resistance mechanisms ***:: Immature stages of *** were collected between March and July 2017 in 13 locations across Cameroon and reared until G1/G2/G3 ***,adult bioassays,and piperonyl butoxide(PBO)synergist assays were carried out according to World Health Organization guidelines.F1534C mutation was genotyped using allele specific polymerase chain reaction in field collected adults(Go)and the polymorphism of the sodium channel gene was ***χ2 test was used to compare the mortality rate between bioassays with insecticides only and bioassays after preexposure to PBO ***: Larval bioassay revealed that all the three populations tested with temephos were *** bioassays showed a good level of susceptibility toward both pyrethroids tested,0.25%permethrin and 0.05%deltamethrin,with six out of 10 populations ***,two populations(Douala and Edéa)were resistant(deltamethrin[73.2–92.5%mortality],permethrin[2.6–76.3%mortality]).The resistance to 4%dichlorodiphenyltrichloroethane was observed in four out of 10 populations tested(16.8–87.1%mortality).Resistance was also reported to carbamates including 0.1%propoxur(60.8–87.1%mortality)and to 0.1%bendiocarb(82.9%mortality).All populations tested were fully susceptible to 1%fenitrothion.A partial recovery of susceptibility was observed in the pyrethroid resistant population of Douala after pre-exposed to PBO suggesting the implication of cytochrome P450 monoxygenases permethrin *** and sequencing detected the F1534C kdr mutation in the two pyrethroid resistant

关键词： Aedes aegypti Insecticide resistance kdr mutation Cameroon Arbovirus

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Correlation between adult pyrethroid resistance and knockdown resistance(kdr)mutations in Aedes albopictus(Diptera:Culicidae)field populations in China

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Infectious Diseases of Poverty 2018年第1期7卷 870-878页

作者： Jing-Peng Gao Han-Ming Chen Hua Shi Heng Peng Ya-Jun Ma Department of Tropical Infectious Diseases Second Military Medical UniversityShanghai 200433China Institute of Disease Control and Prevention People’s Liberation Army of ChinaBeijing 100071China Department of Medical Microbiology and Parasitology College of Basic Medical SciencesSecond Military Medical UniversityShanghai 200433China

Background:Arboviral disease transmitted by Aedes albopictus such as dengue fever is an important threat to human *** resistance raises a great challenge for mosquito control.A systematic assessment of *** resistance status in China is urgently needed,and the study of correlation between pyrethroid resistance and knockdown resistance(kdr)mutations would provide information to guide the control of the *** ***:Five field populations of *** were collected from Jinan(JN),Hangzhou(HZ),Baoshan(BS),Yangpu(YP)and Haikou(HK),China in ***-impregnated papers were prepared with four pyrethroid chemicals,deltamethrin,permethrin,beta-cypermethrin and *** susceptibility of *** to pyrethroids was tested by the WHO tube *** mutations were identified by PCR and ***,the correlation analysis between kdr alleles and pyrethroid resistance was ***:All five populations of *** showed resistance to four pyrethroid *** kdr mutant allele at codon 1532 and three at 1534 were detected with frequency of 5.33%(I1532T),44.20%(F1534S),1.83%(F1534 L)and 0.87%(F1534C),*** 1532 and 1534 mutation mosquitoes were found in the BS and YP *** I1532T was negatively correlated with deltamethrin resistance phenotype(ORmutation was positively correlated with deltamethrin and permethrin resistance(OR>1).Conclusions:The five field populations of *** adults were all resistant to deltamethrin,permethrin,betacypermethrin and *** F1534S was clearly associated with pyrethroid resistance phenotype in *** and this could be developed as a molecular marker to monitor the pyrethroid resistance problem in China.

关键词： Aedes albopictus Pyrethroid Insecticide resistance kdr mutation WHO tube bioassay

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First investigation of deltamethrin pyrethroid susceptibility and resistance status of Anopheles labranchiae(Falleroni, 1926), potential malaria vector in Tunisia

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Asian Pacific Journal of Tropical Biomedicine 2017年第12期7卷 1067-1070页

作者： Ahmed Tabbabi Jabeur Daaboub Department of Hygiene and Environmental Protection Ministry of Public HealthTunisTunisia

Objective: To evaluate the deltamethrin pyrethroid insecticides against Anopheles labranchiae, potential malaria vector in ***: Six field populations of Anopheles labranchiae mosquitoes were collected from six localities in Northern and Central Tunisia between October and November *** bioassays were performed to estimate the level of resistance in each collected population. Two synergists were used to estimate the involvement of detoxification enzymes in insecticide ***: All studied strains were resistant and the RR50 ranged from 12.5 in sample #1 to72.5 in sample #6. Synergist tests using piperonyl butoxide indicated the involvement of monoxygenases enzymes in the recorded resistance. In contrast, the increase of deltamethrin mortality was not significant in presence of S,S,sributyl phosphorothioate(0.8 kdr mutation(Spearman rank correlation, r =-0.59, P < 0.01).Conclusions: These results should be considered in the current mosquitoes control programs in Tunisia. The use of pesticides and insecticides by both agricultural and public health departments in Tunisia should be more rational to reduce the development of resistance in populations. Different insecticide applications should be implemented alternately.

关键词： Resistance Anopheles labranchiae Deltamethrin pyrethroid Synergism kdr mutation Tunisia

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First identification ofallele F1534S in VGSC gene and its association with resistance to pyrethroid insecticides inpopulations from Haikou City, Hainan Island, China

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贫困所致传染病（英文） 2016年第3期

Background: Aedes albopictus is distributed widely in China, as a primary vector of Dengue fever and Chikungunya fever in south of China. Chemical insecticide control is one of the integrated programmes to prevent mosquito-borne diseases. Long-term applications of pyrethroids have resulted in the development of resistance inAe. albopictus populations in China. However, the susceptibility ofAe. albopictus to pyrethroids in Hainan Island was unclear. Knockdown resistance (kdr), caused by point mutations in the VGSC gene, is one of the mechanisms that confer resistance to DDT and pyrethroids. This study was to investigate the resistance level ofAe. albopictus populations in Haikou City to three pyrethroid insecticides, and elucidate the relationship between the resistant phenotype andkdr ***: TheAedes albopictus samples were collected in Xinbu Island (XI), Longtang Town (LT), Shishan Town (ST), Baishamen Park (BP), and Flower Market (FM) from Haikou City, Hainan Island, China. The larval susceptibility to deltamethrin, permethrin and beta-cypermethrin was tested by larval bioassays, and adult susceptibility to deltamethrin and DDT was determined by adult bioassays. The degree of resistance was determined by resistance ratio value (RR50 > 3) for larvae and by mortality for adult. Thekdr alleles at codon 1534 of the VGSC gene were genotyped. The relationship betweenkdr genotypes and resistant phenotypes was analyzed by Chi-square ***: Out of five populations, assessed by larval bioassays, XI was susceptible to deltamethrin and permethrin; LT was susceptible to permethrin and beta-cypermethrin; and ST was susceptible to permithrin. FM and BP both were resistant to all of the three pyrethroids, and FM showed the highest degree of resistance, with RR50 values from 65.17 to 436.36. A total of 493 individuals from the larval bioassays were genotyped forkdr alleles. Five alleles were detected, including two wildtype alleles, TTC(F) (67.04 %) and TTT(F) (

关键词： Aedes albopictus Pyrethroids Resistance kdr mutation China

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