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Experimental study on the effect of kang-lai-te induced apoptosis of human hepatoma carcinoma cell HepG2

Hepatobiliary & Pancreatic Diseases International 2009年第3期8卷 267-272页

作者： Lu, Yun Wu, Li-Qun Dong, Qian Li, Chang-Sheng Qingdao Univ Dept Hepatobiliary Surg Affiliated Hosp Coll Med Qingdao 266003 Peoples R China Qingdao Univ Dept Pediat Surg Affiliated Hosp Coll Med Qingdao 266003 Peoples R China Qingdao Univ Ctr Cell & Mol Pathol Affiliated Hosp Coll Med Qingdao 266003 Peoples R China

BACKGROUND: kang-lai-te (KLT) is extracted from the traditional Chinese herbal medicine Semen Coicis, which has been used in China as an effective clinical drug for over a thousand years. It contains numerous ingredients with anti-tumor effects. In our previous studies on transplanted hepatomas in rats, KLT could stop the cells in the G2+M stage of cell cycle and then reduce the number of cells entering the stage G0 and G1, but the mechanism of the anti-proliferative effect was unknown. In this experiment, we examined whether KLT inhibits HepG2 cell growth, if so, tried to explore its mechanism. METHODS: KLT at different concentrations was used for the treatment of hepatocellular carcinoma cells in vitro, respectively. The proliferation inhibitory rate was evaluated by MTT assay, induction of cell apoptosis rate and the protein levels of Fas and Fas ligand (FasL) were determined by flow cytometry (FCM), and the expression of Fas and FasL mRNA was detected by real-time fluorescent quantitative RT-PCR. RESULTS: KLT produced an obvious time and dose-dependent inhibitory effect on HepG2 cells, and marked apoptosis was detected by FCM The protein of Fas increased by 11.01%, 18.71%, 28.71% and 37.15%; the protein of FasL increased by 1.49%, 1.91%, 3.27% and 3.38% in comparison with the control (Pted through activation of the Fas/FasL pathway. (Hepatobiliary Pancreat Dis Int 2009; 8: 267-272)

关键词： kang-lai-te drugs, Chinese herbal hepatocellular carcinoma HepG2 Fas/FasL

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Efficacy of intra- tumor injection of kang-lai-te in treating transplanted hepatoma in rats

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 580-584页

作者： Li-Qun Wu, Yun Lu, Hua-Jun Lu, Zong-Gang Zhao and Meng Yang Qingdao, China Department of Hepato-biliary-vascular Surgery, Qing- dao University Medical College, Qingdao 266003 , China Department of Hepato-biliary-vascular Surgery Qingdao University Medical College Qingdao 266003 China. jiaoqin@***

BACKGROUND: Non-operative therapy takes an impor- tant position in comprehensive therapy of liver cancer. De- spite some effects by using ethanol, acetic acid and heat sa- line for intra-tumor injection in the treatment of liver canc- er, it is difficult to attain a complete cure but bring about injury to the liver to some extent. Hence, searching for other drugs for the local treatment of liver tumor is an im- portant option. This study was designed to set up rat mo- dels of transplanted liver cancer, intra-tumor injection of kang-lai-te (KLT), and negative control (saline) and positive control (ethanol). The effect of intra-tumor injec- tion of KLT in treating transplanted hepatoma in rats and its advantages and disadvantages were assessed and the pos- sibility of its use in treating patients with liver cancer was evaluated. METHODS: Forty rats were divided into 4 groups ( G1, G2, G3 and G4, 10 rats in each group). Different drugs were injected into their implanted hepatoma (G1 with 0.2 ml saline as control, G2 with 10 mg KLT, G3 with 20 mg KLT, G4 with 0.2 ml ethanol). After 3 and 8 days, the hepa- toma volume (HV), the serum levels of albumin, alanine aminotransferase(ALT), aspartate aminotransferase alkaline phosphatase( ALP) and creatinine, as well as the expression of proliferation cell nuclear antigen (PCNA) in hepatoma were detected. RESULTS: After 3 days, the HVs were smaller in G3 and G4 than in G1 (P ter 8 days, the HVs were smaller in G2, G3 and G4 than in Gl (P <0.05), and the differences of HVs among G2, G3 and G4 were not significant. The serum levels of ALP were lower in G1, G2 and G3 than in G4 (P <0.05), and the PCNA

关键词： kang-lai-te intra-tumor injection transplanted hepatoma rat

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