检索结果-南通市图书馆

Agrobacterium–mediated Transformation of kalanchoe laxiflora

Horticultural Plant Journal 2019年第5期5卷 221-228页

作者： Xiling Wang Xinlu Chen Qunkang Cheng Kaikai Zhu Xiaohan Yang Zongming Cheng Department of Plant Sciences University of TennesseeKnoxvilleTN37996USA College of Biotechnology Southwest UniversityChongqing400715China Department of Entomology and Plant Pathology University of TennesseeKnoxvilleTN37996USA College of Horticulture Nanjing Agricultural UniversityNanjing210095China Biosciences Division Oak Ridge National LaboratoryOak RidgeTN37831-6422USA

Plants with crassulacean acid metabolism(CAM) generally utilize water 20%–80% more efficiently than non-CAM plants. The whole genomes of several CAM plants have been sequenced or are being sequenced. For effective genome characterization and genome editing of CAM plants,an efficient transformation system is essential. In this study, we developed an Agrobacterium-mediated transformation protocol for kalanchoe laxiflora, an obligate CAM plant,by optimizing several factors affecting the transformation efficiency. Agrobacterium strains AGL1, C58, EHA105,and GV3101 were all suitable for K. laxiflora transformation. Fifty-nine percent of the leaf explants yielded kanamycin-resistant and GUS-positive shoots. Polymerase chain reaction and quantitative real-time PCR(qRT-PCR) using gus A-, gus Plus-, npt II-and hpt-specific primers confirmed that the transgenes were integrated into K. laxiflora genome and expressed. This efficient transformation system will allow effective functional characterization of genes through over-or down-expression, knockout, or genome editing.

关键词： kalanchoe laxiflora Agrobacterium-mediated transformation CAM plants

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hypoglycemic and hypocholesterolemic activities of the aqueous preparation of kalanchoe pinnata leaves in streptozotocin-induced diabetic rats

引用

Asian Pacific Journal of Tropical Biomedicine 2015年第1期5卷 3-9页

作者： Nikhil Menon Jean Sparks Felix Omoruyi Department of Life Sciences Texas A&M University-Corpus Christi

Objective:To evaluate the hypoglycemic and hypocholesterolemic activities of the aqueous preparation of kalanchoe pinnata(K.pinnata) leaves in streplozotocin-induced diabetic rats.Methods:Diabetes mellitus was induced in rats by a single administration of streptozotocin(60 mg/Kg).Diabetic rats were then treated with aqueous K.pinnata for 30 d.Serum glucose,proteins.lipid composition,liver and kidney function indices,inflammatory markers,and key enzymes of hepatic carbohydrate and lipid metabolism were determined.Results:The untreated and treated diabetic groups lost weight and consumed less food compared to the normal group.We noted 37.9%decrease in fasting blood glucose in the treated diabetic group compared to 13.2%and 17.0%increases in normal and untreated diabetic groups respectively.Serum cholesterol and triglyceride levels were significantly(P<0.05) reduced in the treated diabetic group compared to the untreated diabetic group.Blood urea nitrogen was significantly(P<0.05) elevated in the untreated and treated diabetic groups compared lo the normal group.Serum alkaline phosphatase and hepatic pyruvate kinase activities were significantly(P<0.05) elevated in the treated diabetic group.Scrum albumin level was signilieantiy(P<0.05) reduced in the untreated diabetic group.Serum IL-6 was significantly(P<0.05) depressed in the treated diabetic group.Conclusions:The observed decrease in body weight,blood glucose and cholesterol level suggests that the aqueous K.pinnata preparation consumption may be beneficial in the management of diabetes mellitus.The observed adverse effect on alkaline phosphatase activity may be due to the combined effect of streptozotocin-induced diabetes and K.pinnata preparation administration.

关键词： kalanchoe pinnata Diabetes Streptozotocin Hypoglycemic Hypocholesterolemic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nutraceutical with Anti-Inflammatory Activity for the Management of Airway Remodeling in Bronchial Asthma: <i>kalanchoe integra</i>Var. Crenata (Andr.) Cuf Leaf Extract

引用

Pharmacology & Pharmacy 2014年第3期5卷 250-261页

作者： Asiedu-Gyekye Isaac Julius Awortwe Charles Nyamekye Effah Samuel Antwi Daniel Ansong Seidu Mahmood Adjei Samuel Banga N’guessan Benoit Kwame Amoateng Patrick Nkansah Edwin Department of Animal Experimentation Noguchi Memorial Institute for Medical Research College of Health Sciences Legon Ghana Department of Chemical Pathology School of Allied Health Sciences College of Health Sciences Korle-Bu Ghana Department of Pharmacology and Toxicology University of Ghana School of Pharmacy College of Health Sciences Legon Ghana Department of Physiology University of Ghana Medical School Col-lege of Health Sciences Korle-Bu Ghana Division of Pharmacology Faculty of Health Sciences University of Stellenbosch Cape Town South Africa

Background: kalanchoe integra is widely used in folklore medicine as an antiasthmatic agent. Previous studies have shown the ameliorating effect of kalanchoe integra leaf extract [KILE] on bronchial hyperesponsiveness and inflammation. Further, the stabilizing effect of kalanchoe sp on mast cell degranulation, suggests that kalanchoe species are suitable candidates for allergic asthma therapy. This study is designed to investigate the anti-asthmatic potential of KILE and monitor the accompanying histopathological and immunobiochemical changes that occur in an animal model of bronchial asthma using ovalbumin sensitized guinea pigs. Method: Thirty male guinea pigs were divided into five groups of six animals each. Bronchial asthma was simulated in guinea pigs using ovalbumin. Both low dose (300 mg/kg) and high dose extract (900 mg/kg) were administered daily for 42 days. Prednisolone (2.5 mg/kg) was the standard drug used. Results: Guinea pigs in all KILE treated groups maintained the integrity of their airway structures: bronchial folds and walls, alveoli, alveolar ducts and sacs. KILE and prednisolone caused a reduction in immune parameters (p 0.001), extent of bronchoconstriction, bronchial wall thickness and goblet cell accumulation in the sensitized guinea pigs. Conclusion: This study demonstrates the anti-asthmatic potential of KILE during prolonged administration by the oral route.

关键词： kalanchoe Integra Asthma Bronchoconstriction Goblet Cells Perivascular Oedema

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of 5'-deletion of Arabi-dopsis profilin2 promoter on its vascular specific expression

引用

Chinese Science Bulletin 2001年第19期46卷 1627-1630页

作者： LIU Yuhui, WANG Zhixing & JIA ShirongBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing China

Based on the published sequence of profilin2 promoter of Arabidopsis thaliana, a full-length promoter (1667 bp) was amplified by PCR. The 5’ -end deletion fragments with length of 1380, 1153, 969 and 597 bp were then fused with gus (uid\) gene respectively. Constructed plant expression vectors were individually transferred into Kalan-choe laciniata and transgenic plants regenerated. GUS his-tochemical assay confirmed that the full-length promoter Pfnl.7 was vascular-specific. Deletion assays showed that profilin2 promoter could be divided into three parts. Deletion of fragment 1 ( -1667 -1380 bp) resulted in constitutive expression, suggesting that element(s) responsible for vascular-specific expression might exist in this region. Fragment 2 located at -1153 - -597 bp strongly inhibited gus gene expression. Fragment 3 ( -597 - -1 bp) is considered as a basic domain of profilin2.

关键词： profilin2 promoter kalanchoe constitutive expression vascular-specific expression.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：