检索结果-南通市图书馆

Journal of Behavioral and Brain Science 2015年第5期5卷 163-172页

作者： Abdul-Karim Abbas Institute of Neuroscience and Physiology University of Gothenburg Gothenburg Sweden

The present study was carried out to investigate the effect of NMDA, bicuculline and kainic acid (KA) on the extracellular concentration of glutathione, phosphoethanolamine (PEA) and taurine in rat hippocampus in vivo. Rats were implanted with intrahippocampal microelectrodes perfused with free-glucose Krebs-Ringer solution and allowed to recover for about 2 h. After assaying baseline concentrations of amino acids, NMDA or bicuculline was administered intrahippocampally, whereas KA was given systemically. Either treatment resulted in significant high extracellular concentrations of glutathione, but only NMDA or KA resulted in high concentrations of PEA and taurine. Interestingly, the increase in glutathione concentration due to KA was followed by a delayed increase of glutamate and PEA. Our results demonstrated that increased efflux of glutathione, a common consequence of different neuroexcitotoxic agents, occurs in vivo. Given that the agents used in the present study were also convulsunts, the implication of the findings on seizure predisposition was also considered.

关键词： NMDA Glutathione Taurine Hippocampus Microdialysis kainic Acid Seizure

Coexistence of nitric oxide synthase with γ-aminobutyric acid during seizure induced by kainic acid in SD rats

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Medical Colleges of PLA(China) 1998年第2期13卷 83-87页

作者：解新荣黄远桂

Objective: Functional significance of NO and central inhibitory neurotransmitter γ-aminobutyric(GABA) during seizures were investigated morphorlogically. Methods: A kainate-induced complex partialseizure model was used in our experiment. Twenty SD rats were randomly divided into KA 30, 60, 90, 200min and control groups. The brain sections were stained by NADPh (nicotinamide adenine dinucleotide phosphate ) diaphorase (Nd ) histochemically, and were further stained by GABA ***: Histological and immunohistochemical study revealed that in KA groups the number of Nd and GABA-positive double labelled neurons in CA3 region, CA3 region and dentate gyms was significantly reduced,compared with the control group. Conclusion: Nd coexisted with GABA in the brain. Reduction of GABA release led to relief of GABA-ergic inhibition and in the same way, reduction of NO release weakened its negative feedback modulation. Therefore neuronal synchronous paroxysmal discharges increased. GABA and NO,both having antiepileptic action, acted through different ways or different link in the same way. NO may involve in the effect of GABA-ergic neurons and play cooperative antiepileptic action with GABA.

关键词： nitric oxide (NO) nitric oxide synthase (NOS) seizures kainic acid (KA) γ-amino-butyricacid (GABA) NADPH diaphorase (Nd)

Time-dependent changes of astrocytes following microinjection of kainic acid and irradiation by gamma-knife in adult rat brain

在线全文

学校读者我要写书评

暂无评论

Journal of Medical Colleges of PLA(China) 1999年第3期14卷 166-170页

作者：杨婷饶志仁鞠躬

objective: Two groups of rats were microinjected with kainic acid (KA) and irradiated with gam ma knife respectively on unilateral nucleus caudate-putamen to compare the response of astrocytes. Methods: The astrocytes were identified with anti-GFAP immunohistochemical ABC method and the progress of their reaction to the 2 insults was examined from 3 h to 30 d after the lesion. Results: Both lesions could induce hyperplasia and hypertrophy of astrocytes and 2 types of GFAP-ir cells were found, one with small cell body and thin process, and the other with hypertrophic cell body and thick and long process. The timecourse of GFAP expression in the 2 groups was different. In KA microinjection group, large necrotic area was ob served in the target within 24 h. Three days later, a few astrocytes appeared around the necrosis. With in crease of the survival time, hyperplasia and hypertrophy of astrocytes began to increase. Whereas in gamma knife group, hyperplasia and hypertrophy were evident from 3 h to 7 d and necrotic dots could be seen in the target on day 14. On day 30, necrosis was tnore obvious with gradual variations in GFAP expression around the necrotic area. Conclusion: The above results indicated that GFAP could be used as a marker for CNS in jury; the difference in their timing and distribution pattern suggested different mechanisms in KA microinjec tion group and gamma-knife irradiation group.

关键词： GFAP kainic acid gamma knife astrocyte

A Study of Epileptiform Hippocampal Unit Activity Induced by Intracerebroventricular Injection of kainic Acid in Rats

在线全文

学校读者我要写书评

暂无评论

Journal of Medical Colleges of PLA(China) 1989年第3期4卷 272-276页

作者：郭庆刘祚周廖维宏 Department of Physiology Third Military Medical College Chongqing

Hippocampal EEG and unit activities were recorded after seizures were initiated withintracerebroventricular injection of kainic acid (KA) in rats. Three kinds of hippocampal unit re-sponses to KA were found:1. Positive units: These units were characterized by high frequency burst firing temporally coincidentwith each hippocampal EEG spike.2. Negative units: These units showed a cessation of firing during each EEG paroxysm.3. Indifferent units: These units showed no evident chanses coincident with EEG *** positive units were hippocampal complex spike cells which correspond histologically tohippocampal pyramidal cells, and most complex spike cells fired in positive bursts after KA treat-ment. In the early period after KA injection, the positive units were concentrated in CA3 area. It was suggested that the activities of positive units may be considered as the typical epileptiformhippocampal unit activity induced by KA, and the firing features of negative units ma be the resultof the influence of hippocampal inhibitory interneurons or the result of excessive cellulardepolarization, and that hippocampal pyramidal cells were more sensitive to the epileptogenic ef-fect of KA than hippocampal intemeurons, and some pyramidal cells in CA3, in particular,may serve as "epileptic pacemaker neurons " in KA-induced epileptogenesis.

关键词： temporal lobe epilepsy kainic acid hippocampus unit activity rat

Dynamic changes of mG1uR1 mRNA expression in rat hippocampus during seizures induced by kainic acid

在线全文

学校读者我要写书评

暂无评论

Journal of Medical Colleges of PLA(China) 2000年第1期15卷 55-59页

作者：江文黄远桂高华肖华胜

Objective: To investigate temporospatial changes of mG1uR1 mRNA expression in rat hippocampus during seizures induced by kainic acid. Methods: Using in situ hybridization with Dig-labeled cDNA probes to examine mG1uR1 mRNA expression. The relative number of hybridization signal was quantified by image analysis systems. EEG and behavior changes of the rats were simultaneously observed. Results: All rats of the KA-injected group exhibited severe limbic convulsions. The EEG recordings also showed electrical seizure activities. The mG1uR1 mRNA expression began to increase at 1 h after KA injection and reached its maximal level at 4 - 8 h, and then it began to decrease gradually and came to the lowest by the end of 72 h. Conclusion: The enhanced early expression of mG1uR1 mRNA in hippocampus suggested that mG1uR1 may play an important role in epilepogenesis and may be related to the subsequent neuronal damage.

关键词： epilepsy metabotropic glutamate receptors hippocampus kainic acid electroencephalogram

Immunohistochemical Analysis of Citrulline-Nitric Oxide Cycle Enzymes and Glutamine Synthetase in Different Regions of Brain in Epilepsy Rat Model

在线全文

学校读者我要写书评

暂无评论

Neuroscience & Medicine 2014年第3期5卷 131-138页

作者： Mummedy Swamy Intan Nurfirdaus Mat Zin K. N. S. Sirajudeen Zulkarnain Mustapha Chandran Govindasamy Department of Chemical Pathology School of Medical Sciences Health Campus Universiti Sains MalaysiaKelantan Malaysia

The aim of this study was to determine the immunoreactivity of neuronal and inducible nitric oxide synthetase, argininosuccinate synthetase, argininosuccinate lyase, glutamine synthetase in different regions of brain in rats of kainic acid mediated epilepsy. Male Sprague-Dawley rats were used in this study. The acute group animals were sacrificed after 2 hours and the chronic group animals were sacrificed after 5 days of a single subcutaneous injection of kainic acid (15 mg/kg body weight). The cerebral cortex, cerebellum and brain stem slices were fixed and immunohistostained for the above enzymes. Images were captured and analyzed. In acute group, argininosuccinate synthetase and inducible nitric oxide synthetase were increased in cerebral cortex and cerebellum, neuronal nitric oxide synthetase increased in cerebral cortex and brain stem, and there was no change in argininosuccinate lyase immunoreactivity compared to control group. In chronic group, glutamine synthetase was decreased and all other enzymes immunoreactivity was increased in all the brain regions tested. This study demonstrated the up-regulation of citrul-line-nitric oxide cycle enzymes and may contribute to enhancing recycling of citrulline to arginine to support the increased production of nitric oxide in epilepsy. The decreased glutamine synthetase may increase glutamate in chronic epilepsy and may lead to neurodegeneration.

关键词： Citrulline-Nitric Oxide Cycle Enzymes Epilepsy Glutamine Synthetase Immunohistochemistry kainic Acid Rat Brain

Changes in α- and β-tubulin gene expressions in hippocampal formation during epileptogenesis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1995年第15期40卷 1282-1286页

作者：郭庆匡培根范明甘思德 Department of Neurology. Instute of Rasic Medicil Sciences Achlicm of Military Meclical Sciences Beijing 100853. China Department of Neurology. PLA General Hospital. Beijing 100853. China Department of Neurobiology. Institute of Basic Medical Sciences. Academy of Military Medical Sciences Beijing 100850 China

kainic acid (KA) is a structural analogue of glutamate with potent excitotoxic andconvulsant properties. Intraperitoneal injection of KA in rats produces recurrentspontaneous limbic seizures accompanied by brain damage reminiscent of Ammon’s hornsclerosis, which has been considered as a useful model of human temporal lobe epilep-

关键词： epilepsy hippocampus kainic acid tubulin mRNA Northern blot analysis.