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Study of Ompk.5 and Ompk.6 Expression in Carbapenem Resistant ESBL Producing Clinical Isolates of k.ebsiella pneumoniae

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Advances in Microbiology 2016年第9期6卷 662-670页

作者： Amina Amal Mahmoud Nour El Din Reem Abdel Hameed Harfoush Hadir Ahmed Said Ok.sha Dina Aly El Sayed k.oleif Department of Medical Microbiology and Immunology Faculty of Medicine Alexandria University Alexandria Egypt

Back.round: Carbapenem resistant extended spectrum β-lactamase (ESBL) producing k.ebsiella pneumoniae (k. pneumoniae) is increasing worldwide. Carbapenem resistance (CR) has been attributed not only to production of carbapenemases but also to permeability barriers due to outer membrane proteins (Ompk.5 and Ompk.6) disruption. Objective: Phenotypic detection of CR among ESBL producing k. pneumoniae isolates, followed by the evaluation of the role of ompk.5 and ompk.6 gene expression among carbapenem resistant k. pneumoniae (CR-k.) isolates. Methods: 100 ESBL producing k. pneumoniae isolates were included in this study. Minimum inhibitory concentration (MIC) of imipenem was performed for all isolates by broth microdilution method. For CR-k. isolates, phenotypic detection of k. pneumoniae carbapenemase (k.C), metallo-β-lactamase (MBL) and AmpC enzymes was performed followed by Realtime qRT-PCR to detect and quantify ompk.5 and ompk.6 gene expression. Results: 42% of our isolates were carbapenem resistant, and all of them were k.C producers either singly or in combination with MBL and/or AmpC production. Reduced expression of both ompk.5 and ompk.6 was detected in (52.38%) of CR-k. isolates, while reduced expression of ompk.6 or ompk.5 alone was found in (2.38%) and (33.33%) respectively. Twenty of 42 CR-k. isolates (47.62%), showing reduced ompk.5 and ompk.6 expression, exhibited high level resistance (HLR) (>32 μg/ml) to imipenem. There was a significant correlation between reduced expression of ompk.6 and increase MIC values (p k.5 and/or ompk.6 resulted in significant increase in imipenem MIC (p k.5/Ompk.6 loss resulted in HLR. However Ompk.6 seems to play a major role in those strains. Imipenem MIC was mark.dly increased among k. pneumoniae showing carbapenemase and/or AmpC production together with loss of Ompk.5 and/or Ompk.6.

关键词： Ompk.5 Ompk.6 k. pneumoniae Carbapenem Resistant

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Isolation and Identification of Alk.li-Resistant 1,3-Propanediol Producing Strain

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Advances in Microbiology 2016年第12期6卷 917-926页

作者： Zhifang Zhao Caifang Wen Guang Rong Ruiqing Liu Jianguo Xu Qingping Hu College of Life Science Shanxi Normal University Linfen China

1,3-Propanediol is a promising renewable resource produced by microbial production. It is mainly used in many synthetic reactions, particularly applied to the polymer synthesis and cosmetics industry. We described here the isolation of strain ZH-1, which has the ability of high production with 1,3-propanediol, from Fenhe River in China. It was classified as a member of k. pneumoniae after the study of phenotypic, physio-logical, biochemical and phylogenetic (16S rDNA). The initial glycerol concentration, fermentation time and pH value of strain ZH-1 were determined to be 50 g·L-1, 36 h and 8.0. Under these conditions, the practical yield of 1,3-PD was 18.53 g·L-1 and a molar yield (mol1,3-PD molGlycerol-1 of 1,3-propanediol to glycerol of 0.497. In addition, we found that for the strain ZH-1, the optimum grown pH was 9.0, so we can deter-mine that it is a new member of alk.li-resistant strains.

关键词： 1 3-Propanediol k. pneumoniae Identification Alk.li-Resistant

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