检索结果-南通市图书馆

Reduced Locomotor Activity Correlates with Increased Severity of Arthritis in a Mouse Model of Antibody-Induced Arthritis

引用

Open Journal of Rheumatology and Autoimmune Diseases 2014年第1期4卷 62-68页

作者： Narendiran Rajasekaran Ricky Tran Conrado Pascual Xinmin Xie Elizabeth D. Mellins AfaSci Research Laboratory Redwood City USA. Department of Pediatrics Program in Immunology Stanford University Medical Center Stanford USA Department of Pediatrics Program in Immunology Stanford University Medical Center Stanford USA

Introduction: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial hyperplasia and progressive cartilage and bone destruction that leads to a substantial loss of general functions and/or a decline in physical activities such as walking speed in humans. The k/bxn serum transfer arthritis in mice shares many immunological and pathological features with human RA. Very few studies are available in mice that investigate the changes in physical activity in relation to arthritis development. In this study we investigate the effect of arthritis on the locomotor activity of mice during k/bxn sera transfer arthritis. Methods: Arthritis was induced in Balb/c mice by injecting intraperitoneally with 200 μl of k/bxn sera;Balb/c mice injected with phosphate buffered saline (PBS) served as control. Progress of arthritis was estimated by daily measurements of joint thickness. Each mouse’s locomotor activity (travel distance and travel time) was assessed every day for duration of 20 minute period using the SmartCageTM platform. Data were analyzed using the SmartCageTM analysis software (CageScoreTM). Results: Arthritic Balb/c mice showed a reduction in distance covered and travel speed when compared to arthritis-free, control Balb/c mice. Maximum decline in locomotor activity was observed during the peak period of the disease and correlated to the increase in joint thickness in the arthritic mice. Conclusion: This report demonstrates that measuring locomotor activity of mice during progression of k/bxn sera-induced arthritis using the SmartCageTM platform offers a quantitative method to assess physical activity in mice during arthritis.

关键词： k/bxn Arthritis Locomotion Serum Transfer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Matrix Metalloproteinase MMP-9 Promotes k/bxn Serum Induced Arthritis in Mice

引用

Open Journal of Rheumatology and Autoimmune Diseases 2014年第1期4卷 22-28页

作者： Narendiran Rajasekaran Harald Illges Department of Natural Sciences Immunology and Cell Biology University of Applied Sciences Rheinbach Germany

Matrix metalloproteinases (MMPs) are matrix-degrading enzymes that are over-expressed in joints of rheumatoid arthritis (RA) patients. However, the contribution of specific MMPs for the development of arthritic joints is unknown. This study is aimed at studying the role of matrix metalloproteinase-9 (MMP-9) in mice, using the k/bxn serum-transfer model of RA. Arthritis was induced in Balb/c mice by injecting k/bxn serum. Development of arthritis was followed in these mice by measuring ankle thickness and clinical index score. MMP-9 expression in the joints of mice killed at various time points during the disease progression was determined by gelatin zymography using ankle lysates. We found that MMP-9 expression increased with the severity of arthritis. Importantly MMP-9 deficient mice injected with k/bxn serum showed a milder form of arthritis in comparison to the control C57BL/6 mice injected with k/bxn serum. We therefore conclude that MMP-9 promotes arthritis in mice.

关键词： MMP-9 Antibody Induced Arthritis k/bxn Gelatin Zymography

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：