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Cell junction proteins within the cochlea: A review of recent research

Journal of Otology 2015年第4期10卷 131-135页

作者： Bo Wang Bohua Hu Shiming Yang Department of Otolaryngology Head&Neck Surgery Institute of Otolaryngology Chinese PLA General Hospital Astronaut Research and Training Center of China Center for Hearing and Deafness State University of New York at Buffalo

Cell-cell junctions in the cochlea are highly complex and well organized. The role of these junctions is to maintain structural and functional integrity of the cochlea. In this review, we describe classification of cell junction-associated proteins identified within the cochlea and provide a brief overview of the function of these proteins in adherent junctions, gap junctions and tight junctions. Copyright ? 2016, PLA General Hospital Department of Otolaryngology Head and Neck Surgery. Production and hosting by Elsevier (Singapore) Pte Ltd. This is an open access article under the CC BY-NC-ND license (http://***/licenses/by-nc-nd/4.0/).

关键词： Cochlea Cell junction junction proteins

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Pericyte-like differentiation of human adipose-derived mesenchymal stem cells:An in vitro study

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World Journal of Stem Cells 2020年第10期12卷 1152-1170页

作者： Giuliana Mannino Florinda Gennuso Giovanni Giurdanella Federica Conti Filippo Drago Salvatore Salomone Debora Lo Furno Claudio Bucolo Rosario Giuffrida Physiology Section Department of Biomedical and Biotechnological SciencesUniversity of CataniaCatania 95123Italy Pharmacology Section Department of Biomedical and Biotechnological SciencesSchool of MedicineUniversity of CataniaCatania 95123Italy Biochemistry Section Department of Biomedical and Biotechnological SciencesSchool of MedicineUniversity of CataniaCatania 95123Italy

BACKGROUND Adipose-derived mesenchymal stem cells(ASCs)are characterized by long-term self-renewal and a high proliferation *** adequate conditions,they may differentiate into cells belonging to mesodermal,endodermal or ectodermal *** support endothelial cells and play an important role in stabilizing the vessel wall at the microcirculation *** loss of pericytes,as occurs in diabetic retinopathy,results in a breakdown of the blood-retina barrier(BRB)and infiltration of inflammatory *** this context,the use of pericytelike differentiated ASCs may represent a valuable therapeutic strategy for restoring BRB *** To test in vitro strategies to obtain pericyte-like differentiation of human ASCs(hASCs).METHODS Different culture conditions were tested:hASCs cultured in a basal medium supplemented with transforming growth factorβ1;and hASCs cultured in a specific pericyte medium(PM-hASCs).In a further sample,pericyte growth supplement was omitted from the *** addition,cultures of human retinal pericytes(hRPCs)were used for ***-like differentiation of hASCs was tested by immunocytochemical staining and western blotting to evaluate the expression ofα-smooth muscle actin(α-SMA)and neural/glial antigen 2(NG2).Interactions between human retinal endothelial cells(hRECs)and different groups of hASCs were investigated in co-culture *** these cases,the expression of typical junctional proteins such as vascular endothelial-Cadherin,zonula occludens-1 and Occludin were assessed in *** an in vitro model of the BRB,values of trans-endothelial electrical resistance were measured when hRECs were co-cultured with various groups of pretreated *** values observed were compared with co-cultures of hRECs and hRPCs as well as with cultures of hRECs ***-dimensional co-cultures of hRECs and hRPCs or pericyte-like hASCs in Matrigel were designed to assess their reciprocal *** After 3-6 d of cult

关键词： Adipose-derived mesenchymal stem cells Pericyte-like differentiation Retinal endothelial cells Retinal pericytes Blood-retina barrier junction proteins

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