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Studies on Heteroses of Pei'ai 64S/javanica rice

Hunan Agricultural Science ＆ Technology Newsletter 2002年第3期3卷 4-10页

作者： Tang Li, Xiao Guoying, Yuan Longping, and Deng XiaoxiangChina National Hybrid rice Research and Development Center, Changsha 410125, China China National Hybrid rice Research and Development Center Changsha 410125 China

Agronomic traits and heteroses of javanica varieties and the indica/javanica hybrids in Changsha are reported. javanica rice exhibits long panicles, big grains, less panicles per plant, a long growth duration and high plant height in Changsha. The hybrid of Pei'ai 64S/javanica possesses positive heterobeltiosis in culm height, panicle length, panicles per plant, spikelets per panicle, grains per panicle, yield per plant, theoretic yield per hectare and actual yield per hectare; performs negative heterobeltiosis in days to flower, seed setting rate and 1000-grain weight. There are significant linear regression relations between culm height of the hybrid and panicle length of its male parent, panicle length of the hybrid and that of its male parent, spikelets per panicle of the hybrid and spikelets per panicle and panicle length of its male parent, grains per panicle of the hybrid and spikelets per panicle of its male parent, 1000-grain weight of the hybrid and spikelets per panicle of its male parent, respectively. In general, significant differences at 1% level are seen in most traits except grains per panicle and theoretic yield per hectare between the indica/javanica hybrid and Pei'ai 64S/Teqin (CK). Compared with the check, the indica/javanica hybrids have positive Standard heterosis in culm height, panicle length, spikelets per panicle, grains per panicle and 1000-grain weight but negative standard heterosis in days to flower, panicles per plant, seed setting rate, theoretic yield per hectare and actual yield per hectare. For the individuals, 11.1% hybrids possess > 40% standard heterosis in theoretic yield per hectare, 3.7% possess >40% standard heterosis in actual yield per hectare. The javanica restorer line 770 derives from a single cross of Pei'ai 64S/Moroberekan B-51-4-9-2. The hybrid 43S/770 possesses an elite plant type and strong heterosis.

关键词： javanica rice Pei'ai 64S heterosis hybrid rice

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Expression analysis of OsSERK,OsLEC1 and OsWOX4 genes in rice(Oryza sativa L.)callus during somatic embryo development

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BIOCELL 2022年第7期46卷 1633-1641页

作者： SITI NABILAH TRI HANDOYO KYUNG-MIN KIM MOHAMMAD UBAIDILLAH Graduate School of Biotechnology University of JemberJember68121Indonesia Faculty of Agriculture University of JemberJember68121Indonesia Center Development of Advanced Science and Technology(CDAST) University of JemberJember68121Indonesia Division of Plant Biosciences School of Applied BioSciencesCollege of Agriculture and Life ScienceKyungpook National UniversityDaegu41566South Korea

Somatic embryogenesis is an asexual reproduction process that occurs in many plant species,including *** process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase(SERK),Leafy Cotyledon1(LEC1)and WUSCHEL-Related Homeobox4(WOX4)and also a helpful model for embryo development and clones and ***,we report the gene expression during somatic embryo development correlates with regeneration frequency in 14 javanica rice(pigmented and non-pigmented)using modified N6 media supplemented with Kinetin(2.0 mg/L)and NAA(1.0 mg/L).Although there have been advances in understanding the genetic basis of somatic embryogenesis in other varieties,rice is still unexplored,especially during somatic embryo ***,for the formation of callus induction from immature embryos,2,4-D(2.0 mg/L,3.0 mg/L)was *** study analysed the gene expression of OsSERK,OsWOX4 and OsLEC1 genes through RT-PCR *** expression of the OsLEC1 gene indicates that their function may correlate in the in vitro with the high response of rice after transfer to regeneration *** study found that rice varieties of pigmented rice(MS Pendek and Gogoniti II)and non-pigmented rice(Pandan Ungu)showed high regeneration frequency,showing higher OsLEC1 expression than other varieties because OsLEC1 promotes the maturation of somatic embryos in plant regeneration on day ***,the contrast with Genjah nganjuk may be effective because of other regulatory ***-PCR analysis showed OsSERK had less expression level than OsLEC1 and OsWOX4 in the varieties,which correlate with the percentage of plant regeneration,but not for Gogoniti *** conclusion,the higher percentage of plant regeneration correlates with the higher expression level of OsLEC1 at day 14 of media regeneration of rice.

关键词： javanica rice OsLEC1 OsSERK OsWOX4 Plant regeneration Somatic embryos

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