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Fluorescence Quenching Investigation for janus green b and used as Probe in Determination of Nucleic Acids

Chinese Journal of Chemistry 2005年第3期23卷 291-296页

作者：陈莉华刘六战沈含熙 DepartmentofChemistry JishouUniversityJishouHunan416000China//DepartmentofChemistryNankaiUniversityTianjin300071China DepartmentofChemistry NankaiUniversityTianjin300071China

Fluorescence quenching of janus green b (JGb) in sodium dodecyl sulfate (SDS) micelle by nucleic acids (DNA) was studied using UV‐vis absorption, steady state fluorescence emission methods and lifetime measurements. In the SDS micelle, weak fluorescence of JGb was enhanced, and the maximum emission shifted from 425 to 410 nm. In the presence of DNA, the fluorescence of JGb was quenched. Linear relationships between the fluorescence quenching ( F 0 /F ) and concentrations of DNA were observed in the range of 2.4×10 −8 to 1.08×10 −7 mol·L −1 for calf thymus nucleic acids (ct DNA) and 1.9×10 −8 to 3.8×10 −8 mol·L −1 for fish sperm nucleic acids (fs DNA) when 2.5×10 −5 mol·L −1 JGb was employed. The limit detection were 1.3×10 −8 mol·L −1 for ct DNA and 6.4×10 −9 mol·L −1 for fs DNA. At high DNA concentration, there was a systematic deviation from the Stern‐Volmer equation due to the static and dynamic quenching occurring simultaneously. The proposed method was applied to the determination of the nucleic acids in chicken blood extraction and the analytical results were in good agreement with the UV‐method.

关键词： janus green b fluorescence quenching micelle determination of nucleic acid

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Interaction between janus green b and bovine serum albumin:Electrochemistry and spectroscopy studies

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Chinese Chemical Letters 2011年第6期22卷 717-720页

作者： Neslihan zdemir Serkan zdemir Ender bier Ondokuz Mayrs University Faculty of Arts and Sciences Department of Chemistry

In this study,voltammetric and spectroscopic investigation of the interaction between janus green b（JGb） and bovine serum albumin（bSA） was *** interaction was observed at britton-Robinson buffer（pH 7.0）.When JGb was added to solution containing bSA,the peak currents of bSA decrease with the increasing of JGb concentrations which is due to the interaction of JGb and *** binding constant of JGb with bSA was obtained by voltammetric ***,this interaction was supported by means of UV-vis spectroscopic *** UV-vis absorption spectra of JGb in the presence of bSA decrease with the increasing of bSA concentrations.

关键词： Voltammetry bovine serum albumin janus green b Interaction

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