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The MYC2-PUB22-jaZ4 module plays a crucial role in jasmonate signaling in tomato

Molecular Plant 2024年第4期17卷 598-613页

作者： Shaofang Wu Chaoyi Hu Changan Zhu Yanfen Fan Jie Zhou Xiaojia Xia Kai Shi Yanhong Zhou Christine H.Foyer Jingquan Yu Department of Horticulture Zijingang CampusZhejiang UniversityHangzhou 310058China Hainan Institute Zhejiang UniversityYazhou Bay Science and Technology CitySanya 572025China College of Horticulture Northwest Agriculture&Forestry UniversityXianyang 712100China School of Biosciences College of Life and Environmental SciencesUniversity of BirminghamEdgbaston B152TTUK Key Laboratory of Horticultural Plants Growth and Development Agricultural Ministry of ChinaZhejiang UniversityHangzhou 310058China

jasmonates(jas),a class of lipid-derived stress hormones,play a crucial role across an array of plant physiological processes and stress responses.Although ja signaling is thought to rely predominantly on the degradation of specific jaZ proteins by SCF^(COI1),it remains unclear whether other pathways are involved in the regulation of jaZ protein stability.Here,we report that PUB22,a plant U-box type E3 ubiquitin ligase,plays a critical role in the regulation of plant resistance against Helicoverpa armigera and other ja responses in tomato.Whereas COI1 physically interacts with jaZ1/2/5/7,PUB22 physically interacts with jaZ1/3/4/6.PUB22 ubiquitinates jaZ4 to promote its degradation via the 26S proteasome pathway.Importantly,we observed that pub22 mutants showreduced resistance to H.armigera,whereas jaz4 single mutants and jaz1 jaz3 jaz4 jaz6 quadruple mutants have enhanced resistance.The hypersensitivity of pub22 mutants to herbivores could be partially rescued by jaZ4 mutation.Moreover,we found that expression of PUB22 can be transcriptionally activated by MYC2,thus forming a positive feedback circuit in ja signaling.We noticed that the PUB22-jaZ4 module also regulates other ja responses,including defense against B.cinerea,inhibition of root elongation,and anthocyanin accumulation.Taken together,these results indicate that PUB22 plays a crucial role in plant growth and defense responses,together with COI1-regulated ja signaling,by targeting specific jaZs.

关键词： PUB22 jaZ COI1 MYC2 ja signaling Solanum lycopersicum

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The Phytophthora effector Avh94 manipulates host jasmonic acid signaling to promote infection

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Journal of Integrative Plant Biology 2022年第11期64卷 2199-2210页

作者： Yao Zhao Bo Yang Huawei Xu Jinbin Wu Zhiyang Xu Yuanchao Wang Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095China College of Grassland Science Nanjing Agricultural UniversityNanjing 210095China

The oomycete pathogen Phytophthora sojae is a causal agent of soybean root rot.Upon colonization of soybeans,P.sojae secretes various RXLR effectors to suppress host immune responses,supporting successful infection.Previous research has demonstrated that the RXLR effector Avh94 functions as a virulence effector,but the molecular mechanism underlying its role in virulence remains unknown.Here,we demonstrate that Avh94 overexpression in plants and pathogens promotes Phytophthora infection.Avh94 interacts with soybean jaZ1/2,which is a repressor of jasmonic acid(ja)signaling.Avh94 stabilizes jaZ1/2 to inhibit ja signaling and silencing of jaZ1/2 enhances soybean resistance against P.sojae.Moreover,P.sojae lines overexpressing Avh94 inhibit ja signaling.Furthermore,exogenous application of methyl jasmonate improves plant resistance to Phytophthora.Taken together,these findings suggest that P.sojae employs an RXLR effector to hijack ja signaling and thereby promote infection.

关键词： effector ja signaling Phytophthora pathogen plant immunity

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ZmMYC2s play important roles in maize responses to simulated herbivory and jasmonate

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Journal of Integrative Plant Biology 2023年第4期65卷 1041-1058页

作者： Canrong Ma Ruoyue Li Yan Sun Mou Zhang Sen Li Yuxing Xu Juan Song Jing Li Jinfeng Qi Lei Wang Jianqiang Wu Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of Botanythe Chinese Academy of SciencesKunming 650201China Chinese Academy of Science Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China

Both herbivory and jasmonic acid(ja)activate the biosynthesis of defensive metabolites in maize,but the mechanism underlying this remains unclear.We generated maize mutants in which ZmMYC2a and ZmMYC2b,two transcription factor genes important in ja signaling,were individually or both knocked out.Genetic and biochemical analyses were used to elucidate the functions of ZmMYC2 proteins in the maize response to simulated herbivory and ja.Compared with the wild-type(WT)maize,the double mutant myc2ab was highly susceptible to insects,and the levels of benzoxazinoids and volatile terpenes,and the levels of their biosynthesis gene transcripts,were much lower in the mutants than in the WT maize after simulated insect feeding or ja treatment.Moreover,ZmMYC2a and ZmMYC2b played a redundant role in maize resistance to insects and ja signaling.Transcriptome and Cleavage Under Targets and TagmentationSequencing(CUT&Tag-Seq)analysis indicated that ZmMYC2s physically targeted 60%of the jaresponsive genes,even though only 33%of these genes were transcriptionally ZmMYC2-dependent.Importantly,CUT&Tag-Seq and dual luciferase assays revealed that ZmMYC2s transactivate the benzoxazinoid and volatile terpene biosynthesis genes IGPS1/3,BX10/11/12/14,and TPS10/2/3/4/5/8 by directly binding to their promoters.Furthermore,several transcription factors physically targeted by ZmMYC2s were identified,and these are likely to function in the regulation of benzoxazinoid biosynthesis.This work reveals the transcriptional regulatory landscapes of both ja signaling and ZmMYC2s in maize and provides comprehensive mechanistic insight into how ja signaling modulates defenses in maize responses to herbivory through ZmMYC2s.

关键词： benzoxazinoid defense insect ja signaling maize MYC2 volatile terpene

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Plant immune inducer ZNC promotes rutin accumulation and enhances resistance to Botrytis cinerea in tomato

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Stress Biology 2023年第1期3卷 387-402页

作者： Haipeng Zhao Xiangyu Ding Xiaomeng Chu Haimiao Zhang Xinyu Wang Xinwen Zhang Haoqi Liu Xiaoying Zhang Ziyi Yin Yang Li Xinhua Ding State Key Laboratory of Crop Biology Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect PestsCollege of Plant ProtectionShandong Agricultural UniversityTaian 271018ShandongP.R.China Shandong Pengbo Biotechnology Co. Ltd.Taian 271000China

Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks.ZNC(zhinengcong),an extract derived from the endophytic fungus Paecilo-myces variotii,has been discovered to play a vital role in preventing diverse forms of bacterial infections.Nevertheless,the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear.In this study,we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B.cinerea.The results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin.Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by B.cinerea.In addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(ja)-mediated pathways.Therefore,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.

关键词： Endophytic fungus extract Metabolome Flavonoids Tomato gray mold ja signaling ROS

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The Glucosinolate Biosynthetic Gene A OP2 Mediates Feed-back Regulation of jasmonic Acid signaling in Arabidopsis

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Molecular Plant 2015年第8期8卷 1201-1212页

作者： Meike Burow Susanna Atwell Marta Francisco Rachel E. Kerwin Barbara A. Halkier Daniel J. Kliebenstein DynaMo Center of Excellence Department of Plant and Environmental Sciences Faculty of Science University of Copenhagen Thorvaldsensvej 40 1871Frederiksberg C Denmark Copenhagen Plant Science Centre Department of Plant and Environmental Sciences Faculty of Science University of Copenhagen Thorvaldsensvej 40 1871Frederiksberg C Denmark Department of Plant Sciences University of California at Davis Davis CA 95616 USA Mision Biologica de Galicia (MBG-CSIC) PO Box 28 36080 Pontevedra Spain

Survival in changing and challenging environments requires an organism to efficiently obtain and use its resources. Due to their sessile nature, it is particularly critical for plants to dynamically optimize their meta- bolism. In plant primary metabolism, metabolic fine-tuning involves feed-back mechanisms whereby the output of a pathway controls its input to generate a precise and robust response to environmental changes. By contrast, few studies have addressed the potential for feed-back regulation of secondary metabolism. In Arabidopsis, accumulation of the defense compounds glucosinolates has previously been linked to genetic variation in the glucosinolate biosynthetic gene AOP2. AOP2 expression can increase the transcript levels of two known regulators （MYB28 and MYB29） of the pathway, suggesting thatAOP2 plays a role in positive feed-back regulation controlling glucosinolate biosynthesis. We generated mutants affecting AOP2, MYB28/29, or both. Transcriptome analysis of these mutants identified a so far unrecognized link between AOP2 and jasmonic acid （ja） signaling independent of MYB28 and MYB29. Thus, AOP2 is part of a regulatory feed-back loop linking glucosinolate biosynthesis and ja signaling and thereby allows the glucosinolate pathway to influence ja sensitivity. The discovery of this regulatory feed-back loop provides insight into how plants optimize the use of resources for defensive metabolites.

关键词： feed-back regulation jasmonates ja signaling glucosinolates Arabidopsis

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Transcriptome Analysis of ja Signal Transduction,Transcription Factors,and Monoterpene Biosynthesis Pathway in Response to Methyl jasmonate Elicitation in Mentha canadensis L.

Transcriptome Analysis of JA Signal Transduction,Transcripti...

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第七届长三角植物科学研讨会暨青年学术报告会

作者： Xiwu Qi Hailing Fang Dongbei Xu Chengyuan Liang Weilin Li Institute of Botany Jiangsu Province and Chinese Academy of Sciences

Mentha canadensis L.has important economic value for its abundance in essential oils.Menthol is the main component of M.canadensis essential oils,which is certainly the best-knownmonoterpene for its simple structure and wide applications.However,the regulation of menthol biosynthesis remains elusive in M.canadensis.In this study,transcriptome sequencing of M.canadensis with Me ja treatment was applied to illustrate the transcriptional regulation of plant secondary metabolites,especially menthol biosynthesis.Six sequencing libraries were constructed including three replicates for both control check(CK) and methyl jasmonate(Meja) treatment and at least 8 Gb clean bases was produced for each library.After assembly,a total of 81,843 unigenes were obtained with an average length of 724 bp.Functional annotation indicated that 64.55% of unigenes could be annotated in at least one database.Additionally,4430 differentially expressed genes(DEGs) with 2383 up-regulated and 2047 down-regulated transcripts were identified under Meja treatment.Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment indicated that "Monoterpenoid biosynthesis" was one of the most significantly enriched pathways in metabolism.Subsequently,DEGs involved in ja signal transduction,transcription factors,and monoterpene biosynthesis were analyzed.9 orthologous genes involved in menthol biosynthesis were also identified.This is the first report of a transcriptome study of M.canadensis and will facilitate the studies of monoterpene biosynthesis in the genus Mentha.

关键词： Mentha canadensis L. transcriptome sequencing ja signaling transcription factors menthol biosynthesis

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Arabidopsis IQM1 is involved in stomatal innate immune

Arabidopsis IQM1 is involved in stomatal innate immune

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广东省遗传学会第九届代表大会暨学术研讨会

作者：周玉萍 Qiong-Hua Chen Xiao-Ling Huang Zhang-Ke Huang Zhong-Zhen Mo 田长恩 School of life Sciences Guangzhou University

Arabidopsis IQMs share sequence homology with a pea heavy metal-induced protein 6 and a trichosanthin.Distinct expression patterns for each gene suggest that each IQM family member may play a different role in plant development and response to environmental cues.However functions of the IQM family members remain to be analyzed.IQMl can bind with AtCaM5 in yeast two-hybrid assay via its IQ-motif.The CaM binding was Ca-independent in vitro,and was also observed in bimolecular fluorescence complementation analyses in onion epidermal cells.IQMl was found to express strongly in guard cells and the cortex of roots.The T-DNA insertion mutants of IQMl(iqml) displayed a smaller stomatal aperture,a decreased water loss rate and a shorter primary root.Moreover,iqml did not change its stomatal aperture when treated with light,dark,ABA and chitin obviously.Stomatal guard cell of iqml-1 also showed enhanced expression of genes involved in production and signaling of reactive oxygen species(ROS).Consistently,increased ROS level was observed in guard cells.Interestingly,243 and 28 genes were up- and down-regulated by more than two fold in the leaf of iqml-1,respectively.Particularly,34 of 117 and 7 of 30 chitin-responsive transcriptional factor and ubiquitin ligase genes were up-regulated.Furthermore 51defense-associated genes in iqml-1 were also up-regulated.Consistent with above,iqml-1 was found to be more resistant to Pst DC3000 than wild type when the pathogen is sprayed,but to show similar symptom to wild type when the pathogen is infiltrated,suggesting that IQMl is involved in stomatal innate immune.Our further results showed that mutation of IQMl reduces sensitivity to J A which enhances stomatal aperture and consequently reduces resistance to Pst DC3000,indicating that IQMl enhance J A signal transduction and stomatal open and consequently reduce resistance to Pst DC3000.

关键词： AtIQM1 Stomatal movement Reactive oxygen species Calmodulin-binding protein ja signaling Arabidopsis thaliana Stomatal innate immune Pst DC3000

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