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Antidepressant-Like Effects of Liquiritin and isoliquiritin in the Forced Swimming Test and Tail Suspension Test in mice

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Antidepressant-Like Effects of Liquiritin and Isoliquiritin ...

Intestinal permeability of liquiritin and isoliquiritin in the Caco-2 cell monolayer model

2007中药药物创新与制药关键技术研讨会暨中华中医药学会中药化学中药制剂学术会议

作者： Dean Guo School of Pharmaceutical Sciences Peking University

Two classical behavioral despair animal models—the Forced Swimming Test (FST) and the Tail Suspension Test(TST) in mice were used to evaluate the antidepressant activity of liquiritin and isoliquiritin from Glycyrrhiza *** was observed that liquiritin and isoliquiritin could significantly reduce the immobility time in the FST and TST in mice. The measurement of locomotor activity indicated that liquiritin and isoliquiritin have no central nervous system(CNS)-stimulating effects.

关键词： Antidepressant Forced swimming test Glycyrrhiza uralensis isoliquiritin Liquiritin Neuro-transmitters Tail suspension test

来源： cnki会议评论

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cnki会议

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Journal of Chinese Pharmaceutical Sciences 2010年第6期19卷 451-458页

作者：张力勤黄应栋杨秀伟北京大学医学部天然药物及仿生药物国家重点实验室药学院天然药物学系北京100191

The intestinal permeability of two flavonoid compounds liquiritin （LQ） and isoliquiritin （ILQ） was investigated using the Caco-2 cell monolayer model. In order to evaluate the permeability and predict the absorption mechanism of the two compounds, the study on bidirectional permeability from the apical （AP） side to the basolateral （BL） side as well as from the BL side to the AP side was carried out. The determination was performed by HPLC-UV method. And the permeability parameters, especially the apparent permeability coefficients （Papp）, were then calculated. The Papp values of LQ and ILQ are （5.40±0.16）× 10^-7 and （8.69±0.15）× 10^-7 cm/s, respectively. The results of time- and concentration-dependent transport experiments indicate that both LQ and ILQ are poor absorbed mainly through passive diffusion.

关键词： Liquiritin isoliquiritin Caco-2 cell monolayer model Intestinal permeability

De novo biosynthesis of liquiritin in Saccharomyces cerevisiae

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Acta Pharmaceutica Sinica B 2020年第4期10卷 711-721页

作者： Yan Yin Yanpeng Li Dan Jiang Xianan Zhang Wei Gao Chunsheng Liu School of Chinese Pharmacy Beijing University of Chinese MedicineBeijing 102488China School of Traditional Chinese Medicine Capital Medical UniversityBeijing 100069China School of Pharmaceutical Sciences Advanced Innovation Center for Human Brain ProtectionCapital Medical UniversityBeijing 100069China Pharmacy Department Beijing Longfu HospitalBeijing 100010China

Liquiritigenin(LG),isoliquiritigenin(Iso-LG),together with their respective glycoside derivatives liquiritin(LN)and isoliquiritin(Iso-LN),are the main active flavonoids of Glycyrrhiza uralensis,which is arguably the most widely used medicinal plant with enormous demand on the market,including Chinese medicine prescriptions,preparations,health care products and even *** studies have shown that these ingredients have broad medicinal value,including anti-cancer and antiinflammatory *** the biosynthetic pathway of glycyrrhizin,a triterpenoid component from ***,has been fully analyzed,little attention has been paid to the biosynthesis of the flavonoids of this *** obtain the enzyme-coding genes responsible for the biosynthesis of LN,analysis and screening were carried out by combining genome and comparative transcriptome database searches of *** and homologous genes of known flavonoid biosynthesis *** catalytic functions of candidate genes were determined by in vitro or in vivo *** work characterized the complete biosynthetic pathway of LN and achieved the de novo biosynthesis of liquiritin in Saccharomyces cerevisiae using endogenous yeast metabolites as precursors and cofactors for the first time,which provides a possibility for the economical and sustainable production and application of *** flavonoids through synthetic biology.

关键词： Glycyrrhiza uralensis Liquiritin isoliquiritin Liquiritigenin Isoliquiritigenin Heterologous synthesis Saccharomyces cerevisiae

Neuroprotective effect of isoliquriitin on corticosterone-induced apoptosis in PC12 cells

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中国药理学与毒理学杂志 2017年第5期31卷 467-467页

作者： LI Xiao GONG Wen-xia ZHOU Yu-zhi QIN Xue-mei Modern Research Center for Traditional Chinese Medicine Shanxi University College of Chemistry and Chemical Engineering Shanxi University

OBJECTIVE isoliquiritin,a flavonoid glycosides compound,possess a broad spectrum of pharmacological activities including antioxidant,antiinflammatory. However,rare studies in the field of isoliquiritin antidepressant-like effects have been carried out,the molecular mechanism also remains unclear. In this study,the model of corticosterone-damaged rat adrenal pheochromocytoma(PC12)cells has been used to generate an in vitro experimental model of depression. The aim was to investigate thecytoprotective efficiency and potential mechanisms of isoliquiritin in corticosterone-damaged PC12 cells. METHODS The cells were treated with 400 μmol·L^(-1)corticosterone in the absence or presence of isoliquiritin for 24 h; cells viability and lactate dehydrogenase(LDH) leakage were then determined,Hoechst 33342/PI and Annexin V/PI double staining were performed to evaluate the cytoprotective efficiency of isoliquiritin. Next,intracellular calcium([Ca^(2+)]_i),mitochondrial membrane potential(MMP),reactive oxygen species(ROS),malondialdehyde(MDA),the activity of superoxide dismutase(SOD)and catalase(CAT),and Western blotting analysed for the expression of Bcl,Bax,caspase-3 and cytochrome C(Cyt-C) were investigated to explore the potential mechanisms. RESULTS The results of the present study showed that pretreatment of PC12 cells with isoliquiritinsignificantly prevented the corticosterone-induced cell apoptosis. In addition,isoliquiritin increased the activity of SOD and CAT,decreased the contents of ROS and MDA. These findings suggest that isoliquiritin provided a protective action against corticosterone-induced celldamage by reducing oxidative stress. Furthermore pretreatmented with isoliquiritin reduced corticosterone-induced mitochondrial dysfunction by preventing mitochondrial membrane potentials *** findings indicated that isoliquiritin might exert its therapeutic effects viaregulating mitochondrial dysfunction. Moreover isoliquiritin strongly attenuated [Ca^(2+)]_i

关键词： isoliquiritin neurotoxicity oxidative stress mitochondria apoptotic calcium