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Characterization of cytotoxic compound from mangrove derived fungi irpex hydnoides VB4

Asian Pacific Journal of Tropical Biomedicine 2011年第3期1卷 223-226页

作者： B Valentin Bhimba DA Agnel Defora Franco Geena Mary Jose Jibi Merin Mathew Elsa Lycias Joel Department of Biotechnology Sathyabama University

Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove ***:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated *** search sequence similarity was found against the existing non redundant nucleotide sequence database thus,identified as Aspergilus flavus,Hyporcaea lixii,Aspergillus niger,Eutorium amstelodami,irpex hydnoides and Neurospora *** the seven isolates, one fungi irpex hydnoides was selected for further *** fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies. Results:Nearly half(49.25%) of the extracts showed activity(IC_(50) of 125 μ g/mL).These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity(IC_(50)<20 μ g/mL) in the screening of crude plant *** GC MS analysis revealed that the active principals might be Tetradecane(6.26%) with the RT ***:It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical,anti cancer screening *** results help us conclude that me potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off.

关键词： Mangrove fungi Cytotoxic activity ITS sequencing GC MS irpex hynoides Characterization Cytotoxic compound Endophytic fungi Mangrove Bioactive metabolite

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