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American Journal of Plant Sciences 2015年第9期6卷 1519-1526页

作者： Mohammad I. Al-Masri Sahar M. Sharawi Radwan M. Barakat Plant Protection Research Center (PPRC) Faculty of Agriculture Hebron University Hebron Palestine

The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg·ml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg·ml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.

关键词： Inula viscose Gray Mould Medical Plant Extract iprodione

新杀菌剂扑海因(iprodione)田间药效试验结果简报

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植物保护 1982年第3期 23-24页

作者：黄增灿北京市植保站

扑海因商品名为ROVRAL,系法国罗纳普朗克农业化学公司七十年代产品。据介绍对葡萄孢属(Botrytis)、核盘属(Sclerotinia)、小核属(Sclerotiorum)、链孢霉属(Monilia)、丝核属(Rhizoctonia)、链格(交链)孢属(Alternaria)蠕孢霉属(Helminthosporiunr)、茎点属(Phoma),腥黑粉属(Tilletia)、镰刀菌属(Fusariurn)等均有效。为了鉴定其在生产上的实用价值,1981年针对生产上存在的病害问题,采用50％扑海因可湿性粉剂(ROVRAL50WP)

关键词：小麦拌种剂扑海因多菌灵保卫田杂环类杀菌剂 iprodione 小麦根腐病可湿性粉剂农药剂型田间药效试验

Biocontrol Efficacy of Two Antagonistic Yeasts Against Postharvest Diseases in Peach Fruits During Storage Periods

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Agricultural Sciences in China 2003年第11期2卷 1227-1231页

作者： LIN Li, TIAN Shi-ping, QIN Guo-zheng and XU Yong(Key Laboratory of Photosynthesis and Environmental Molecular Physiology , Institute of Botany, Chinese Academy of Sciences , Beijing 100093 , P.R.China) Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 P. R. China

Two antagonistic yeasts, Thichosporon pullulans and Cryptococcus laurentii, were investigated for their biocontrol potential to blue mold rot and rhizopus rot on harvested peach fruits(Prunus persica L. Batsch, cv. Okubao), alone or in combination with a low dose of iprodione(50 μg ml-1). The results indicated that T. pullulans and C. laurentii were effective at reducing disease incidence and severity of blue mold rot and rhizopus rot in peach fruits. Biocontrol efficacy of C. laurentii and T. pullulans were significantly enhanced by combination with a low dose of iprodione(50 μg ml-1)against blue mold and rhizopus rot in peach fruits. T. pullulans and C. laurentii combined with a low dose of iprodione(50 μg ml-1)resulted in better disease control than either iprodione or the yeasts used alone. Dipping fruits in suspensions of antagonist cells showed the similar control effect as the treatment with iprodione(500μg ml-1).

关键词： Peach fruits Postharvest disease Antagonistic yeasts Biocontrol iprodione

Biomolecular characterization, identification, enzyme activities of molds and physiological changes in sweet potatoes(Ipomea batatas) stored under controlled atmospheric conditions

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第4期17卷 317-332页

作者： C.O.OLADOYE I.F.CONNERTON R.M.O.KAYODE P.F.OMOJASOLA I.B.KAYODE Division of Food Sciences School of Biosciences the University of Nottingham Sutton Bonington Campus Division of Food Preservation Microbial Biotechnology and Toxicology Department of Home Economics and Food ScienceUniversity of Ilorin Department of Microbiology Faculty of Life Sciences University of Ilorin

Microbial attacks during storage are one of the primary causes of product deterioration, and can limit the process of prolonging the shelf-life of harvested food. In this study, sweet potatoes were stored at temperatures of 13, 21, and 29 ℃ for 4 weeks. Samples were collected during storage and plated on potato dextrose agar, from which axenic mold cultures were obtained and identified using 26S rRNA gene sequences. Physiological changes of potato tubers were assessed with respect to pathogenicity, enzyme activity, and atmospheric storage conditions. Six fungal species were identified, namely Penicillium chrysogenum （P. rubens）, P. brevicompactum, Mucor circinelloides, C/adosporium cladosporiodes, P. expansum, and P. crustosum. The following fungal isolates, namely P. expansum, P. brevicompactum, and Rhizopus oryzae, were recovered from the re-infected samples and selected according to their levels of enzyme activity. This study revealed high levels of activity for cellulase and pectinase, which were most notable during the initial three days of testing, and were followed by a steady decrease （P〈0.05）. Polygalacturonase activity was prominent with values ranging from 12.64 to 56.79 U/mg （P. expansum） and 18.36 to 79.01 U/mg （P. brevicompactum）. Spoilage was obvious in the control group, which had a 100% decay at the end of the experimental period compared with samples treated with iprodione and sodium hypochlorite, in which the decay rates were 5% and 55%, respectively. The data for the iprodione- and sodium hypochlorite-treated samples at the end of the 3-month storage period showed that they were significantly different （P=0.041）, with the sodium hypochlorite-treated samples producing twice the rate of infection compared to the iprodione-treated samples. The comparative rate of the pro- gression of decay in the treated samples can be expressed as iprodione〈sodium hypochlorite〈control. This study demonstrates that sweet potato tissue damage is due to the activities

关键词： Controlled atmosphere Enzyme activity Molds characterization Potato spoilage Sodium hypochlorite iprodione

Effects of Trichosporon sp. in Combination with Calcium and Fungicide on Biocontrol of Postharvest Diseases in Apple Fruits

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Acta Botanica Sinica 2001年第5期43卷 501-505页

作者：田世平范青徐勇汪沂中国科学院植物研究所北京100093

The capability of yeast Trichosporon sp., an antagonist isolated from peach fruit, in biological control was evaluated in apple ( Malus domestica Borkh. cv. Fuji) fruits, when inoculated with different concentrations of Botrytis cinerea Pers. and Penicillium expansum (Link) Thom, as well as in combination with calcium and fungicide. The concentrations of the yeast cells and pathogen spores obviously influenced disease incidence and lesion development in apples. There was a significant negative correlation between concentrations of the yeast cells and infectivity of the pathogens. When the yeast cell suspensions reached the concentration of 10 8 colony_forming units (CFU) /mL, there was no infection caused by B. cinerea and P. expansum with spore concentrations below 10 6 spores/mL in apple fruits. The yeast at concentrations of 10 6-10 7 CFU/mL in combination with fungicide (iprodione at 50 μL/L) provided control of decay caused by B. cinerea and P. expansum better than separate application. Effect of controlling gray mould and blue mould rots was enhanced when Trichosporon sp., even at low concentration of 10 5 CFU/mL, was applied in the presence of 1%-2% CaCl 2 in an aqueous suspension.

关键词： Trichosporon sp. calcium iprodione postharvest diseases apple

Fresh keeping techniques for purple cabbage

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Agricultural Science & Technology 2004年第3期5卷 22-25页

作者：黄绍力黄健新徐勋志常绍东广州市农业科学研究所广东广州510308

The cold storage period of purple cabbage packaged with the respirat ory cling film may last 150 days at 2℃by dipping the root with 200×iprodione. Normal temperature storage and quick-freezing storage(-18℃) are not... 详细信息

关键词： purple cabbage iprodione low temperature storage fresh keeping