检索结果-南通市图书馆

intracytoplasmic sperm injection Fertilization Rate Does Not-Depend on the Proportion of Round-headed sperm, Small acrosomal sperm, or Morphologically Normal sperm in Patients with Partial Globozoospermia

引用

Chinese Medical Journal 2015年第12期128卷 1590-1595页

作者： Ling-Ying Jiang Ling-Yun Yang Xiao-Mei Tong Hai-Yan Zhu Ya-Mei Xue Wen-Zhi Xu Yang Yang Song-Ying Zhang Department of Obstetrics and Gynecology Assisted Reproduction Unit Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou Zhejiang 310016 China

Background：Generally,intracytoplasmic sperm injection （ICSI） may be the preferable method to treat partial globozoospermia,but whether there exist some correlations between ICSI fertilization rate and the proportion of round-headed sperm or morphologically normal sperm remains *** study was to explore the correlation between ICSI fertilization rate and the sperm morphology in patients with partial ***：Thirty-four patients diagnosed with partial globozoospermia accepted the following assisted fertilization treatments-2 cases accepted in-vitro fertilization （IvF） alone,26 cases accepted ICSI alone,and 6 accepted split IVF/*** morphological characteristics were described using Diff-Quik rapid *** cases accepting IVF or ICSI treatment in our reproductive center were considered as the control group after being matched by relevant *** rate,embryo quality,embryo implantation rate and clinical pregnancy rate were ***：Besides very high proportion of round-headed sperm,partial globozoospermia also showed very high proportion of small-acrosomal sperm and very low proportion of morphologically normal *** rate of IVF （IVF alone plus split IVF） was very low in partial globozoospermia （25.4% ± 17.4%）,but ICSI （ICSI alone plus split ICSI） achieved satisfying fertilization rate compared with the control group （66.2% ± 22.5% vs.68.8% ± 29.4%,P 〉 0.05）.In patients with partial globozoospermia,there were no correlations between ICSI fertilization rate and the proportion of round-headed sperm,small-acrosomal sperm,or morphologically normal ***：There was high proportion of small-acrosomal sperm in partial *** patients with partial globozoospermia,ICSI is more preferable than *** fertilization rate does not depend on the proportion of round-headed sperm,small-acrosomal sperm,or morphologically normal sperm.

关键词： Fertilization Rate Globozoospermia intracytoplasmic sperm injection In-vitro Fertilization sperm Acrosome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Use of testicular sperm in couples with SCSA-defined high sperm DNA fragmentation and failed intracytoplasmic sperm injection using ejaculated sperm

引用

Asian Journal of Andrology 2020年第4期22卷 348-353页

作者： Mohannad Alharbi Fadl Hamouche Simon Phillips Jacques Isaac Kadoch Armand Zini Division of Urology Department of SurgeryMcGill UniversityMontreal H4A3J1Canada Department of Urology College of MedicineQassim UniversityQassim 1162Saudi Arabia OVO Fertility Clinic Montreal H4P2S4Canada Department of Obstetrics and Gynecology University of MontrealMontreal H3C3J7Canada

sperm DNA fragmentation(SDF)has been linked with male infertility,and previous studies suggest that SDF can have negative influence on pregnancy outcomes with assisted *** performed a retrospective review of consecutive couples with a high SDF level that had intracytoplasmic sperm injection(ICSI)using testicular sperm(T-ICSI).We compared the T-ICSI outcomes to that of two control groups:87 couples with failed first ICSI cycle and who had a second ICSI cycle using ejaculated sperm(Ej-ICSI),and 48 consecutive couples with high sperm chromatin structure assay(SCSA)-defined SDF(>15%)that underwent an ICSI cycle using ejaculated sperm after one or more failed ICSI cycles(Ej-ICSI-high SDF).The mean number of oocytes that were retrieved and the total number of embryos were not different among the three *** mean number of transferred embryos in the T-ICSI group was higher than the Ej-ICSI group but not significantly different than the Ej-ICSI-high SDF group(1.4,1.2,and 1.3,respectively,P0.05).No significant difference was found in live birth rate when comparing T-ICSI to Ej-ICSI and Ej-ICSI-high SDF *** results suggest that pregnancy outcomes and live birth rates with T-ICSI are not significantly superior to Ej-ICSI in patients with an elevated SCSA-defined sperm DNA fragmentation and prior ICSI failure(s).

关键词： intracytoplasmic sperm injection male infertility sperm chromatin structure assay sperm DNA fragmentation sperm retrieval testicular sperm

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparison of intracytoplasmic sperm injection(ICSI)outcomes in infertile men with spermatogenic impairment of differing severity

引用

Asian Journal of Andrology 2022年第3期24卷 299-304页

作者： Ping Ping Zhong Zheng Yi Ma Sha-Sha Zou Xiang-Feng Chen Center for Reproductive Medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200135China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200135China Shanghai Human sperm Bank Shanghai 200135China

The extent of spermatogenic impairment on intracytoplasmic sperm injection(ICSI)outcomes and the risk of major birth defects have been little *** this study,we evaluated the relationship between various spermatogenic conditions,sperm origin on ICSI outcomes,and major birth defects.A total of 934 infertile men attending the Center for Reproductive Medicine of Ren Ji Hospital(Shanghai,China)were classified into six groups:nonobstructive azoospermia(NOA;n=84),extremely severe oligozoospermia(esOZ;n=163),severe oligozoospermia(sOZ,n=174),mild oligozoospermia(mOZ;n=148),obstructive azoospermia(OAZ;n=155),and normozoospermia(NZ;n=210).Rates of fertilization,embryo cleavage,high-quality embryos,implantation,biochemical and clinical pregnancies,abortion,delivery,newborns,as well as major birth malformations,and other newborn outcomes were analyzed and compared among *** NOA group showed a statistically lower fertilization rate(68.2%vs esOZ 77.3%,sOZ 78.0%,mOZ 73.8%,OAZ 76.6%,and NZ 79.3%,all Psperm source or spermatogenic *** failure and sperm origins do not impinge on the clinical outcomes in ICSI treatment.

关键词： intracytoplasmic sperm injection nonobstructive azoospermia normozoospermia obstructive azoospermia oligozoospermia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pregnancies established through intracytoplasmic sperm injection (ICSI) using spermatozoa with dysplasia of fibrous sheath

引用

Asian Journal of Andrology 2000年第2期2卷 125-130页

作者： Santiago Brugo Olmedo Vanesa Y.Rawe Florencia N.Nodar German D.Galavema Anibal A.Acosta Hector E.Chemes Centro de Estudios en Ginecologia y Reproduccion CEGyRBuenos AiresArgentina Laboratory of Testicular Physiology and Pathology Endocrinology DivisionChildren's HospitalBuenos AiresArgentina

Aim:Dysplasia of the fibrous sheath(DFS)is an anomaly found in asthenozoospermic patients with extremely lowor absent *** order to determine the efficacy of ICSI in these patients,a retrospective analysis of ICSI results inDFS patients has been ***:Ten ICSI attempts were performed in 6 patients with diagnosis of Dysplasiaof the Fibrous Sheath studied by transmission and scanning electron ***:In the cases studied,sperm concentration was(29.62±18.05)×10^(6)/mL,total motility was 1.14±1.31%.Progressive motility was 0%except for one case with 0.1%.One hundred and three preovulatory oocytes were obtained and 94 metaphaseⅡoocyteswere ***-nine of them showed two pronuclei(fertilization rate:73.4%).Forty-nine embryos were ob-tained and 34 were transferred(mean:3.4 embryos per transfer).Five pregnancies were diagnosed byβ-hCG plasmalevel determinations that resulted to be one preclinical abortion,one clinical abortion and three ***(ongoing)was achieved from a cryopreserved embryo ***:These results showed thatICSI provides a suitable solution for patients suffering from irreversible sperm defects such as ***,it ismandatory to inform couples of possible transmission risks to offspring,which are unknown at *** when theetiology of this problem is disclosed,it will be possible to assess the real genetic risk.

关键词： spermatozoa dysplasia ciliary motility disorders intracytoplasmic sperm injection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of sperm morphology on pregnancy outcome and offspring in in vitro fertilization and intracytoplasmic sperm injection:a matched case-control study

引用

Asian Journal of Andrology 2021年第4期23卷 421-428页

作者： Wen-Jun Zhou Chuan Huang Su-Hua Jiang Xi-Ren Ji Fei Gong Li-Qing Fan Wen-Bing Zhu Institute of Reproductive and Stem Cell Engineering Basic Medicine CollegeCentral South UniversityChangsha 410008China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410008China Clinical Research Center for Reproduction and Genetics in Hunan Province Changsha 410008China

sperm morphology was once believed as one of the most predictive indicators of pregnancy outcome in assisted reproductive technology(ART).However,the impact of teratozoospermia on in vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI)outcomes and its offspring remains *** order to evaluate the influence of teratozoospermia on pregnancy outcome and newborn status after IVF and ICSI,a retrospective study was *** was a matched case-control study that included 2202 IVF cycles and 2574 ICSI cycles and was conducted at the Reproductive and Genetic Hospital of CITIC-Xiangya in Changsha,China,from June 2013 to June *** were divided into two groups based on sperm morphology:teratozoospermia and normal sperm *** pregnancy outcome and newborn outcome were *** results indicated that couples with teratozoospermia had a significantly lower optimal embryo rate compared to those with normal sperm morphology in IVF(P=0.007),while there were no statistically significant differences between the two groups in terms of the fertilization rate,cleavage rate,implantation rate,and pregnancy rate(all P>0.05).Additionally,teratozoospermia was associated with lower infant birth weight in multiple births after *** regard to ICSI,there was no significant difference in both pregnancy outcome and newborn outcome between the teratozoospermia and normal groups(both P>0.05).Furthermore,no increase in the risk of birth defects occurred in the teratozoospermia group after IVF/***,we believe that teratozoospermia has limited predictive value for pregnancy outcomes in IVF/ICSI,and has little impact on the resulting offspring if multiple pregnancy is avoided.

关键词： in vitro fertilization intracytoplasmic sperm injection newborn outcome pregnancy outcome teratozoospermia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第5期14卷 372-381页

作者： Li-ya WANG Ning WANG Fang LE Lei LI Le-jun LI Xiao-zhen LIU Ying-ming ZHENG Hang-ying LOU Xiang-rong XU Xiao-ming ZHU Yi-min ZHU He-feng HUANG Fan JIN Key Laboratory of Reproductive Genetics (Zhejiang) Ministry of Educationand Centre of Reproductive Medicine Women's HospitalSchool of MedicineZhejiang University Department of Gynecologic Oncology Henan Cancer Hospital

intracytoplasmic sperm injection (ICSI) is commonly used to solve male infertility problems. Previous studies showed that early environmental exposure of an embryo may influence postnatal development. To detect whether ICSI operations affect the reproductive health of a male or his offspring, we established assisted reproductive technologies (ART) conceived mouse models, and analyzed gene expression profiles in the testes of both ICSI and naturally conceived (NC) newborn F1 mice using micro-array analysis. Among the differentially expressed genes, we focused on the expression of eight male reproduction-related genes. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was used to analyze the expression of these genes in the testes of both adult and old F1 generation mice and adult F2 generation mice. Our results showed that down-regulated and somatic cell-expressed genes in newborn mice retained their differential expression patterns in adult and old F1 generation individuals, implying the persistence and fetal origin of the alteration in the expression of these genes. The intergenerational transmission of differential gene expression was observed, but most changes tended to be reduced in adult F2 generations. Controlled ovarian hyperstimulation (COH) and in vitro fertilization (IVF) mice models were added to explore the precise factors contributing to the differences in ICSI offspring. The data demonstrated that superovulation, in vitro culture, and mechanical stimulation involved in ICSI had a cumulative effect on the differential expression of these male reproductive genes.

关键词： intracytoplasmic sperm injection Testis Intergenerational transmission

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Use of testicular sperm for intracytoplasmic sperm injection in men with high sperm DNA fragmentation： a SWOT analysis

引用

Asian Journal of Andrology 2018年第1期20卷 1-8页

作者： Sandro C Esteves Matheus Roque Nicolas Garrido ANDROFERT Andrology and Human Reproduction Clinic Referral Center for Male Reproduction Av. Dr. Heitor Penteado 1464 Campinas SP 13075-460 Brazil ORIGEN Center for Reproductive Medicine Rio de Janeiro Brazil Andrology Laboratory and sperm Bank IVI Foundation Valencia Spain

spermatozoa retrieved from the testis of men with high levels of sperm DNA fragmentation （SDF） in the neat semen tend to have better DNA quality. Given the negative impact of SDF on the outcomes of Assisted Reproductive Technology （ART）, an increased interest has emerged about the use of testicular sperm for intracytoplasmic sperm injection （Testi-ICSI）. In this article, we used a SWOT （strengths, weaknesses, opportunities, and threats） analysis to summarize the advantages and drawbacks of this intervention. The rationale of Testi-ICSI is bypass posttesticular DNA fragmentation caused by oxidative stress during sperm transit through the epididymis. Hence, oocyte fertilization by genomically intact testicular spermatozoa may be optimized, thus increasing the chances of creating a normal embryonic genome and the likelihood of achieving a live birth, as recently demonstrated in men with high SDF. However, there is still limited evidence as regards the clinical efficacy of Testi-ICSI, thus creating opportunities for further confirmatory clinical research as well as investigation of Testi-ICSI in clinical scenarios other than high SDF. Furthermore, Testi-ICSI can be compared to other laboratory preparation methods for deselecting sperm with damaged DNA. At present, the available literature supports the use of testicular sperm when performing ICSI in infertile couples whose male partners have posttesticular SDF. Due to inherent risks of sperm retrieval, Testi-ICSI should be offered when less invasive treatments for alleviating DNA damage have failed. A call for continuous monitoring is nonetheless required concerning the health of generated offspring and the potential complications of sperm retrieval.

关键词： intracytoplasmic sperm injection male infertility sperm DNA fragmentation sperm retrieval SWOT analysis testicular sperm

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Patients with MMAF induced by novel biallelic CFAP43 mutations have good fertility outcomes after intracytoplasmic sperm injection

引用

Asian Journal of Andrology 2023年第5期25卷 564-571页

作者： Jing Ma Shun-Hua Long Hai-Bing Yu Ye-Zhou Xiang Xiang-Rong Tang Jia-Xun Li Wei-Wei Liu Wei Han Rong Jin Guo-Ning Huang Ting-Ting Lin Center for Reproductive Medicine Women and Children's Hospital of Chongqing Medical UniversityChongqing 400010China Chongqing Health Center for Women and Children Chongqing 400010China Chongqing Key Laboratory of Human Embryo Engineering Chongqing 400010China

As a specific type of asthenoteratozoospermia,multiple morphological abnormalities of the sperm flagella(MMAF)is characterized by composite abnormalities,including absent,short,coiled,angulation,and irregular-caliber *** in cilia-and flagella-associated protein 43(CFAP43)are one of the main causative factors of MMAF established to *** identify whether there are other CFAP43 mutations related to MMAF and to determine the clinical outcomes of assisted reproductive technology for patients with MMAF harboring different mutations,we recruited and screened 30 MMAF-affected Chinese men using a 22-gene next-generation sequencing *** systematic analysis,seven mutations in CFAP43,including five novel mutations and two previously reported mutations,were identified from four families and related to MMAF in an autosomal recessive *** staining,immunofluorescence,and electronic microscopy further clarified the semen characteristics a nd abnormal sperm morphologies,including disorganized axonemal and peri-axonemal structures,of the CFAP43-deficient *** female partners of two patients were pregnant after undergoing assisted reproductive technology through intracytoplasmic sperm injection,and one of them successfully gave birth to a healthy *** study significantly expands the mutant spectrum of CFAP43,and together with the available information regarding male infertility and MMAF,provides new information for the genetic diagnosis and counseling of MMAF in the future.

关键词： asthenoteratozoospermia CFAP43 intracytoplasmic sperm injection MMAF

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

sperm origins and concentration do not impact the clinical outcomes in intracytoplasmic sperm injection cycles

引用

Asian Journal of Andrology 2018年第5期20卷 454-458页

作者： Cen Yang Ze-Hong Zhou Dan-Ni Zheng Xiao-Fei XU Jin Huang Ying Lian Jie Qiao Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology Beijing 100191 China Key Laboratory of Assisted Reproduction Ministry Of Education Beijing 100191 China

In the present study, we evaluated the impact of sperm origins and concentration on the clinical outcomes of intracytoplasmic sperm injection （ICSI） cycles. A total of 1201 IC51 cycles were retrospectively analyzed for male azoospermia or oligozoospermia between January 2015 and December 2015 in the Peking University Third Hospital. Patients were divided into three groups （Group 1 vs Group 2/3; surgically extracted sperm vs ejaculated sperms）： Group 1 included 343 ICSI cycles and Group 2 analyzed 388 cycles on semen with sperm concentration 〈5 × 10^6 m1-1 （severe oligozoospermia group）. Group 3 included 470 cycles with sperm concentration between 5×10^6 m1-1 and 15 × 10^6 m1-1 （mild oligozoospermia group）. Fertilization rates, clinical pregnancy rates, and live birth rates were analyzed and compared among groups of different semen origins and concentrations on the oocyte retrieval day. Group 2 showed a lower fertilization rate than Group 3 （62.9%± 21.6% vs 66.8% ± 22.1%, P 〈 0.05）. There were no statistically significant differences in clinical pregnancy rate per transfer （51.3%, 46.7%, and 50.0%, respectively）, live birth rate per transfer （44.4%, 40.9%, and 41.4%, respectively）, accumulative live birth rate （58.3%, 51.0%, and 52.1%, respectively）, twin birth rate （18.4%, 10.6%, and 12.6%, respectively）, and birth defects rate （0, 0.3%, and 0.2%, respectively） among three groups. The results of this study indicated that sperm origins and concentration do not imDact the clinical outcomes in ICSI cycles.

关键词： azoospermia clinical outcomes intracytoplasmic sperm injection oligozoospermia sperm concentration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The hamster egg penetration test may decrease intracytoplasmic sperm injection utilization while maintaining high conventional fertilization rates

引用

Asian Journal of Andrology 2021年第1期23卷 11-15页

作者： Yetunde Ibrahim Brett Einerson Douglas T Carrell Benjamin R Emery Erica Johnstone Division of Reproductive Endocrinology and Infertility Department of Obstetrics and GynecologyUniversity of UtahSchool of MedicineSalt Lake CityUT 84132USA Division of Maternal-Fetal Medicine Department of Obstetrics and GynecologyUniversity of UtahSchool of MedicineSalt Lake CityUT 84132USA Division of Urology Department of SurgeryUniversity of UtahSchool of MedicineSalt Lake CityUT 84132USA Department of Human Genetics University of UtahSchool of MedicineSalt Lake CityUT 84132USA

This was a cohort study of in vitro fertilization(IVF)subjects at the University of Utah,Salt Lake City(UT,USA)utilizing partner *** where both the hamster egg penetration test(HEPT)and semen analysis were performed within 2 years prior to IVF cycles were stratified into four groups based on a normal or an abnormal HEPT and *** mean conventional and intracytoplasmic sperm injection(ICSI)fertilization rates were calculated in each *** performed a univariate analysis on the primary outcome comparing clinically interesting *** performed a cost-effectiveness analysis of a policy of HEPT versus universal ICSI in couples with an abnormal *** patients with a normal HEPT,there was no difference in the mean conventional fertilization rates between those with a normal and an abnormal *** was no difference in the mean conventional fertilization rates between subjects with a normal morphology without a hamster test and those with a normal HEPT without a morphology *** 1000 simulated cycles with an abnormal morphology,a policy of HEPT was cost saving compared to universal ICSI,yet produced similar fertilization *** HEPT is similar to the World Health Organization edition 5(WHO-5)morphology in predicting successful conventional fertilization while allowing decreased utilization of ICSI.A policy of HEPT for males with abnormal morphology saves cost in selecting couples for a fertilization method.

关键词： conventional fertilization hamster egg penetration test intracytoplasmic sperm injection semen analysis sperm penetration assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：