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Effect of lactulose on establishment of a rat non-alcoholic steatohepatitis model

World Journal of Gastroenterology 2005年第32期11卷 5053-5056页

作者： Jian-Gao Fan Zheng-Jie Xu Guo-Liang Wang Department of Gastroenterology Shanghai First People's Hospital Jiaotong University Shanghai 200080 China

AIM： To explore the relationship between changes of intestinal environment and pathogenesis of non-alcoholic steatohepatitis （NASH）. METHODS： Forty-two Sprague-Dawley rats were randomly dMded into model group （n = 24）, treatment group （n = 12）, and control group （n = 6）. The rats of model and treatment groups were given high-fat diet, and those of the control group were given normal diet. Furthermore, the rats of treatment group were given lactulose after 8 wk of high-fat diet. Twelve rats of the model group were killed at 8 wk of high-fat diet. At the 16 wk the rats of treatment group, control group, and the rest of the model group were killed. The serum levels of aminotransferase were measured and the histology of livers was observed by H＆E staining. RESULTS： The livers of rats presented the pathological features of steatohepatitis with higher serum levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in the model group after 16 wk. Compared to the model group, the serum levels of ALT and AST in treatment group decreased significantly and were close to the normal group, and the hepatic inflammation scores also decreased markedly than those in the model group after 16 wk （5.83±2.02 vs 3.63±0.64, P〈0.05）, but were still higher than those in the model group after 8 wk （3.63±0.64 vs 1.98±0.90, P〈0.05）. However, the degree of hepatic steatosis had no changes in treatment group compared to the model group after 16 wk. CONCLUSION： Lactulose could ameliorate the hepatic inflammation of rats with steatohepatitis induced by fat- rich diet, but could not completely prevent the development of steatohepatitis. It is suggested that intestinal environmental changes such as intestinal bacteria overgrowth, are one of the important factors in the pathogenesis of NASH.

关键词： NASH Lactulose intestinal environment

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intestinal microecology associated with fluoride resistance capability of the silkworm（Bombyx mori L.）

Intestinal microecology associated with fluoride resistance ...

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第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会

作者： Guan-Nan Li Xue-Juan Xia Wen-Chao Tang Yong Zhu College of Biotechnology Southwest University

The silkworm（Bombyx mori L.） is an ideal model of Lepidoptera. However, the diversity and function of the intestinal microbiota in the gut of silkworm remain largely unknown. Changes in the intestinal microecology in fluoride-resistant strain T6 and fluoride-susceptible strain 734 of the silkworm in response to fluoride exposure were investigated. T6 and 734 were treated with 200 mg/kg fluoride（designated as T6-T and 734-T groups） and deionized water（designated as T6-C and 734-C groups）. Culture-dependent approach revealed that the numbers of intestinal bacteria in the 734-T group significantly decreased compared with that in the 734-C group（4.8 ± 0.6 × 107 CFU/m L vs. 7.5± 0.7 × 107 CFU/m L;P ＜ 0.05）. Analyses of the intestinal con-tent p H showed that the p H decreased in the 734-T group only. Additionally, SCFA concentrations significantly decreased in both treatment groups compared with the control groups. High-throughput sequencing indicated that the intestinal mi-crobiota in the 734-T group was significantly more diverse than those in the other groups. The bacterial community was composed of two dominant groups（Firmicutes and Proteobacteria）. Principal component analyses revealed a sig-nificant difference in the composition of the intestinal micro-biota in the 734-T group compared with those in the other groups. Thaumarchaeota and Euryarchaeota were more abundant in the 734-T group, but they were less abundant in the other groups. This study enhances our understanding about the diversity and function of silkworm intestinal micro-biota in response to fluoride exposure among silkworm strains with diverse resistance.

关键词： Silkworm(Bombyx mori L.) Fluoride-resistance capability intestinal environment intestinal microbiota Illumina Miseq

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