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Analysis of intestinal microorganisms in Triplophysa rosa(Teleostei,Cypriniformes)

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Analysis of Intestinal Microorganisms in Triplophysa rosa(Te...

Effects of Probiotics on Breeding Environment and intestinal microorganisms of Livestock and Poultry

第八届中国西部动物学学术研讨会

作者： LIU Ni PENG Zuogang School of Life Sciences Southwest University

Triplophysa rosa is a typical species of cave-dwelling fish that distributed throughout Wulong County,Chongqing,China,but the study about its intestinal microorganisms has not been *** purpose of this study was to analyze the structure and diversity of intestinal microorganisms in ***,and explore the function of the intestinal *** extracted total intestinal DNA of ***,then used Illumina Miseq sequencing technology to sequence the V3-V4 region of 16 S r RNA of intestinal *** number of operational taxonomic units(OTUs) of intestinal microorganisms was counted to analyze species composition,abundance and Alpha *** function of intestinal microorganisms was predicted by PICRUSt.A total of1031 OTUs of the high quality sequences were obtained.32 phyla,60 classes,129 orders,231 families,and 502 genera were identified as the major taxonomic *** the phylum level,Proteobacteria,Firmicutes,and Bacteroidetes were dominant in the intestinal *** the genus level,Aeromonas,Edwardsiella,Plesiomonas,and Shewanella were the main *** prediction showed that most of the genes encoded by intestinal microorganisms were related to metabolism,and the relative abundance of the functional flora of "amino acid transport and metabolism" and "carbohydrate transport and metabolism" was relatively *** are complex microorganisms in the intestinal tract of ***,and their functions are conducive to the in-depth understanding of the influence of intestinal microorganisms on the diet,providing a theoretical basis for further studies on the feeding habits of the ***.

关键词： Triplophysa rosa intestinal microorganisms diversity function

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Animal Husbandry and Feed Science 2022年第1期14卷 50-53页

作者： Pan Huiqing Zeng Yan Zhang Yanda Shi Shaohua Yang Rongping Zhao Qi Ningde Enterprise Technology Center of Fujian Beidi Pharmaceutical Co. Ltd.Ningde 355300China Ningde Zherong Environmental Monitoring Station Ningde 355300China Institute of Animal Science and Veterinary Medicine Fujian Academy of A gricultural SciencesFuzhou 350013China

[Objectives] The paper was to study the effects of probiotics on breeding environment of livestock and poultry and intestinal microorgan-isms of breeding animals. [Method] Chicken houses and hog houses were selected as the research objects. The breeding house supplemented with basal diet was used as the control group;the breeding house sprayed with probiotics product 1 was experimental group 1(exogenous treatment);the basal diet mixed with probiotics product 2 and drinking water mixed with product 3 was experimental group 2(endogenous treatment);and the com-bination of the two experimental groups was experimental group 3. [Results] Experimental groups 1 and 2 reduced the concentration of ammonia in hog houses and chicken houses, and the effect of experimental group 2 was better than that of experimental group 1. Experimental group 3 had the best effect, which significantly reduced the ammonia concentration in hog houses and chicken houses by 28.67% and 34.09%, respectively, and the average ammonia concentration outside the house within the range of 100-300 m was less than 2 mg/m^(3). Moreover, it significantly increased the number of intestinal lactobacillus and reduced the number of Escherichia coli. [Conclusions] Probiotics can effectively reduce the ammonia concentration in livestock and poultry breeding environment, and improve the intestinal microbial structure of breeding animals. The technology of spraying exogenous probiotics combined with the use of content-derived probiotics has great potential for popularization and application.

关键词： Probiotics Livestock and poultry Breeding environment intestinal microorganisms

Inhibition of anaerobic probiotics on colorectal cancer cells using intestinal microfluidic systems

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Science China Chemistry 2018年第8期61卷 1034-1042页

作者： Lin Zhou Sifeng Mao Qiushi Huang Xiangwei He Jin-Ming Lin College of Biological Sciences and Biotechnology Beijing Forestry University Beijing Key Laboratory of Microanalytical Methods and Instrumentation Department of Chemistry Tsinghua University

intestinal flora play an important role in human's immune system. Many bacteria adhere to the wall of the testinal wall. These intestinal flora help digestion, and also stop their disease-causing counterparts from invading. Most of the current researches focused on the interaction between cells and the construction of organs, but few researches studied on the role of microorganisms and cells. Here, we developed an in vitro living cell systems to simulate the structure, absorption, transport and pathophysiological characteristics of the human intestinal tract and the key microbial symbiosis. The co-culture of Clostridium butyricum(***) and colon cancer cells showed a different immune effect. C. butyricum could inhibit the proliferation of HCT116 cells, cause cell cycle arrest and promote apoptosis. But it had no significant effect on Caco-2 cells. Thus, basic functional characteristics of the gut were successfully simulated in a controlled microfluidic system. This approach is suggested as a powerful method in the investigation on drug metabolism and intestinal diseases.

关键词： microfluidics intestinal microorganisms co-culture metabolism

Phasic study of intestinal homeostasis disruption in experimental intestinal obstruction

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World Journal of Gastroenterology 2014年第25期20卷 8130-8138页

作者： Xiang-Yang Yu Chang-Lin Zou Zhen-Li Zhou Tao Shan Dong-Hua Li Nai-Qiang Cui Department of Gastrointestinal Surgery Nankai Hospital Graduate School of Tianjin Medical University Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine Department of Hepatopancreatobiliary Surgery Nankai Hospital

AIM: To investigate the phasic alteration of intestinal homeostasis in an experimental model of intestinal obstruction.

关键词： intestinal obstruction Rabbit model Homeostasis disruption intestinal epithelial cells intestinal microorganisms intestinal immune system

Influence of Gut Microbiota and its Metabolites on Progression of Metabolic Associated Fatty Liver Disease

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Chinese Medical Sciences Journal 2023年第4期38卷 286-296页

作者： Yuan-Yuan Wang Hai-Lian Lin Ke-Lang Wang Gen-Xiang Que Ting Cao La-Mei Zhu Xia Yang Xue-Feng Yang Hunan Provincial Clinical Research Center for Metabolic Associated Fatty Liver Disease Affiliated Nanhua HospitalHengyang Medical School&University of South ChinaHengyangHunan 421002China Department of Gastroenterology Affiliated Nanhua HospitalHengyang Medical School&University of South ChinaHengyangHunan 421002China Department of Hepatobiliary Surgery Affiliated Nanhua HospitalHengyang Medical School&University of South ChinaHengyangHunan 421002China Department of General Practice Affiliated Nanhua HospitalHengyang Medical School&University of South ChinaHengyangHunan 421002China

Metabolic associated fatty liver disease(MAFLD)has become a prevalent chronic liver disease worldwide because of lifestyle and dietary *** microbiota and its metabolites have been shown to play a critical role in the pathogenesis of *** of the function of gut microbiota and its metabolites in MAFLD may help to elucidate pathological mechanisms,identify diagnostic markers,and develop drugs or probiotics for the treatment of *** we review the pathogenesis of MAFLD by gut microbiota and its metabolites and discuss the feasibility of treating MAFLD from the perspective of gut microbes.

关键词： metabolic associated fatty liver disease intestinal microorganisms non-alcoholic fatty liver disease liver fibrosis hepatocellular carcinoma

The Use of Restriction Fragment Length Polymorphism and Fluorescence in Situ Hybridization to Investigate Microbiota of Piglets after Feeding Oregano

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Food and Nutrition Sciences 2014年第17期5卷 1628-1636页

作者： Katrin Stelter Andreas Berk Lutz Geue Stefanie Barth Petra Schlien Alexander Swidsinski Sven Danicke Institute of Animal Nutrition Friedrich-Loeffler-Institute(FLI)Federal Research Institute for Animal HealthBraunschweigGermany Institute of Molecular Pathogenesis Friedrich-Loeffler-Institute(FLI)Federal Research Institute for Animal HealthJenaGermany Charite Hospital CCMLaboratory for Molecular GeneticsPolymicrobial Infections and Bacterial BiofilmsDepartment of MedicineGastroenterologyUniversitatsmedizin BerlinBerlinGermany

A total of 80 piglets (7.9 ± 1.0 kg) were used in a feeding experiment with dried oregano. The diets differed in their oregano content: 0 g, 2 g, 4 g and 8 g oregano/kg feed, corresponding to 0, 23.5, 46.9 and 93.9 mg carvacrol/kg DM. After the experimental period of 5 weeks, 20 piglets of both extreme feeding groups were slaughtered: 10 animals of the control group and 10 animals of the group that received 8 g oregano/kg. Ingesta samples of jejunum, caecum and colon were collected and analyzed by FISH and PCR RFLP to compare the diversity of microbiota. The results showed no significant changes in microbiota in response to oregano. The patterns of the PCR-RFLP showed a similarity of 61.8% - 91.8% in both feeding groups. In conclusion, an effect of oregano on the in- testinal microbiota could not be shown under the methods used.

关键词： Piglets Origanum vulgare L. Fluorescence in Situ Hybridization Restriction Fragment Length Polymorphism intestinal microorganisms