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Combined detections of interleukin 27, interferon-γ, and adenosine deaminase in pleural effusion for diagnosis of tuberculous pleurisy

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Chinese Medical Journal 2013年第17期126卷 3215-3221页

作者： WU Yan-bin YE Zhi-jian QIN Sou-ming WU Cong CHEN Yi-qiang SHI Huan-zhong Institute of Respiratory Diseases First Affiliated Hospital Guangxi Medical University Nanning Guangxi 530021 China Department of Respiratory and Critical Care Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Respiratory and Critical Care Medicine Beijing Chao- Yang Hospital Capital Medical University Beijing 100020 China

Background Previous studies reported interleukin-27 （IL-27）, interferon-y （IFN-γ）, or adenosine deaminase （ADA） alone plays a helpful role in diagnosing tuberculous pleural effusion （TPE）. The present study aims at comparing the diagnostic accuracy of pleural IL-27, IFN-γ, and ADA, and investigate the diagnostic accuracy of the combination of IL-27, IFN-γ, or/ and ADA for differentiating TPE from pleural effusions with the other etiologies. Methods The concentrations of IL-27, IFN-γ and ADA were simultaneously determined in pleural fluids and sera from 40 patients with TPE; 26 with malignant pleural effusion, seven with infectious pleural effusion, and eight with transudative pleural effusion by enzyme linked immunosorbent assay and colorimetric method. The corresponding biochemical indexs were also simultaneously determined. Results The concentrations of pleural IL-27 and IFN-γ in the tuberculous group were significantly higher than those in the malignant, infectious, and transudative groups. The concentrations of ADA in TPE were significantly higher than those in MPE or transudative effusions, while much lower than those in infectious effusions. Among these three biomarkers, IL- 27 was the most effective for TPE diagnosis, with the cut off value of 900.8 ng/L. IL-27 had a high sensitivity of 95% and specificity of 97.6% for differential diagnosis of TPE from non-TPEs. Combinations of IL-27, IFN-γ and ADA measurements further increased the sensitivity or specificity up to 100%. Conclusions Compared to non-TPEs, IL-27, IFN-γ and ADA all simultaneously increased in TPE; and among these three rapid detection methods, IL-27 appeared to be the best for distinguishing tuberculous from non-TPEs, especially from MPE. Combinations of the three markers （IL-27, IFN-γ and ADA） yielded the highest sensitivity and specificity. These findings suggest that the applications of a new biomarker, IL-27, alone or with IFN-γ and ADA, may contribute to more efficient diagnosis strategies

关键词： adenosine deaminase interferon-γ interleukin 27 tuberculous pleurisy

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interleukin-27 signaling promotes innate antiviral response in macrophages during early virus infection

Interleukin-27 signaling promotes innate antiviral response ...

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浙江省免疫学会第十三次学术大会

作者： Tianqi Jiang Danhui Liu Lihua Lai Qingqing Wang Institute of Immunology Zhejiang University School of Medicine

Background:interleukin-27（IL-27） is a member of the IL-6/12 superfamily cytokines,previous studies on the antiviral function of IL-27 mainly focused on the adaptive immune response mediated by T cells,whether IL-27 also participate in innate antiviral immune response in macrophages still needs to be further ***:This study aims to investigate the role of interleukin-27 signaling in innate antiviral response in *** and methods:We evaluated the antiviral activity of IL-27 with the Vesicular stomatitis virus（VSV）-infected macrophages,and cells from interferon α/β receptor 1 knockout(Ifnar1) or interleukin 27 receptor a knockout(Wsx-1) ***:Vesicular stomatitis virus（VSV） infection rapidly increased the expression of both subunits of IL-27 in mouse peritoneal *** level of IL-27 in the peripheral blood of mice was positively correlated with the VSV infection *** was observed that pretreatment with recombinant mouse IL-27 cytokine（rmIL-27） to peritoneal macrophages significantly enhanced the resistance to VSV by macrophages,significantly decreased the mRNA level of intracellular viruses,and the viral load in the culture supernatant of ***-1（interleukin 27 receptor a） deficient mice(Wsx-1) showed an increased viral load and mortality rate when compared with wild type（WT）mice upon VSV ***-27 signaling was activated very early stage of VSV infection and was independent on type Ⅰ IFN because the antiviral effect of IL-27 still exists in macrophages derived from Ifnar1 ***,IL-27 also induces IL-10 production during VSV infection in macrophages,Wsx-1 mice showed higher levels of pro-inflammatory cytokines after viral *** study demonstrated that IL-27 is an important component of innate antiviral immune *** mediates type Ⅰ interferons independent innate antiviral activity during the early stage of virus infection in *** IL-27 signaling increases host su

关键词： interleukin 27 antiviral innate immunity Type Ⅰ IFN independent response early stage of virus infection

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Mesenchymal stem cells promoted type 1 regulatory T cells for the treatment of Sj?gren's syndrome via regulating IL-27 production by dendritic cells

Mesenchymal stem cells promoted type 1 regulatory T cells fo...

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第十一届全国免疫学学术大会

作者： Yao Genhong Qi Jingjing Feng Ruihai Song Hua Chen Weiwei Tang Xiaojun Li Wenchao Wang Dandan Hou Yayi Sun Lingyun Department of Rheumatology and Immunology The Affiliated Drum Tower Hospital of Nanjing University Medical School The State Key Laboratory of Pharmaceutical Biotechnology Division of Immunology Medicine SchoolNanjing University and Jiangsu Key Laboratory of Molecular Medicine

Objective:Primary Sj?gren’s syndrome(pSS)is a systemic autoimmune disorder characterized by lymphocytic infiltration of salivary and lachrymal gland leading to dry mouth and eye dryness *** of today’s therapies are ... 详细信息

关键词： Mesenchymal stem cells Primary Sj?gren syndrome Type 1 regulatory T cell interleukin 27

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