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Human iFN-k inhibited Respiratory RNA Virus Replication Dependent on Cell-to-Cell interaction in the Early Phase

infectious Diseases & immunity 2022年第2期2卷 65-73页

作者： Weihui Fu Peng Sun Jun Fan Longfei Ding Songhua Yuan Guanxing Zhai Miaomiao Zhang Chenli Qiu Shuye Zhang Xiaoyan Zhang Jianqing Xu Shanghai Public Health Clinical Center&institutes of Biomedical Sciences Fudan UniversityShanghai 201508China

Background:interferon kappa(iFN-k)is a type i interferon(iFN-i)that inhibits virus replication by evoking interferon-stimulated genes(iSGs).However,as an evolutionarily ancient interferon,iFN-k may function differently from the later emerged interferon-a and ***:Conventional molecular biology methods were used to determine the localization of iFN-k and its structure and *** addition,we employed RT-PCR,western blot,and RNA-Seq technologies to characterize the iSGs expression profile and antiviral activities exerted by iFN-k or ***:Human iFN-k exists in two forms upon ectopic expression,one located on the cell membrane and the other secreted outside the *** membrane-anchored iFN-k showed the ability to induce iSGs and curtail RNA virus replication,whereas the secreted iFN-k failed to do *** analyses indicated that 1-27aa at the N-terminus was the signal peptide,and 28-37aa was predicted as the transmembrane ***,our data demonstrated that both of them were not associated with membrane localization of iFN-k;the former influenced the expression and secretion of iFN-k,and the latter had an impact on the induction of *** addition,prokaryotic purified soluble mature human iFN-k was also capable of inducing iSGs and inhibiting RNA virus ***,human iFN-k induced a faster iSG response but with a lower intensity and a shorter half-life than the response of *** contrast,iFN-a2 started to function later but was stronger and more durable than ***:Human iFN-k-induced iSG response and inhibited respiratory RNA virus replication dependent on cell-to-cell *** addition,compared with iFN-a2,iFN-k exerted effects more rapidly in the early phase,with less intensity and a shorter ***,iFN-k may constitute the first line of iFN-i against respiratory virus infections.

关键词： interferon type i iFN-k influenza Mechanism SARS-CoV-2

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