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The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome-derived signaling in plant development

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Journal of Integrative Plant Biology 2018年第7期60卷 591-607页

作者： Tong Su Pingping Wang Huijuan Li Yiwu Zhao Yao Lu Peng Dai Tianqi Ren Xiaofeng Wang Xuezhi Li Qun Shao Dazhong Zhao Yanxiu Zhao Changle Ma Shandong Provincial Key Laboratory of Plant Stress College of Life Sciences Shandong Normal University Department of Biological Sciences University of Wisconsin-Milwaukee

Hydrogen peroxide （H2O2） is generated in many metabolic processes. As a signaling molecule, H2O2 plays important roles in plant growth and development, as well as environmental stress response. In Arabidopsis, there are three catalase genes, CAT1, CAT2, and CAT3. The encoded catalases are predominately peroxisomal pro- teins and are critical for scavenging H2O2. Since CAT1 and CAT3 are linked on chromosome 1, it has been almost impossible to generate cat1/3 and cats1/2/3 mutants by traditional genetic tools. In this study, we constructed cat1/3 double mutants and cat1/22/3 triple mutants by CRISPR/Cas9 to investigate the role of catalases. The cat1/ 2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditionscompared with wild-type and the cat2/3 double mutants. Transcriptome analysis showed a more profound tran- scriptional response in the cat1/2/3 triple mutants compared to the cat2/3 mutants. These differentially expressed genes are involved in plant growth regulation as well as abiotic and biotic stress responses. In addition, expression of OXI1 （OXIDATIVE SIGNAL INDUCIBLE 1） and several MAPK cascade genes were changed dramatically in the catalase triple mutant, suggesting that H2O2 produced in peroxisomes could serve as a peroxisomal retrograde signal.

关键词： Cong-Ming Lu institute of botany CAS China

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Plant G proteins interact with endoplasmic reticulum luminal protein receptors to regulate endoplasmic reticulum retrieval

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Journal of Integrative Plant Biology 2018年第7期60卷 541-561页

作者： Shanshan Wang Ke Xie Guoyong Xu Huarui Zhou Qiang Guo Jingyi Wu Zengwei Liao Na Liu Yan Wang Yule Liu MOE Key Laboratory of Bioinformatics Center for Plant Biology Tsinghua-Peking Center for Life Sciences School of Life Sciences Tsinghua University Beijing 100084 China Advanced Biotechnology and Application Research Center School of Chemistry and Biological Engineering University of Science and Technology Beijing Beijing 100083 China The institute for Advanced Studies (IAS) Wuhan University Wuhan 430072 China

Maintaining endoplasmic reticulum （ER） homeostasis is essential for the production of biomolecules. ER retrieval, i.e., the retrograde transport of compounds from the Golgi to the ER, is one of the pathways that ensures ER homeostasis. However, the mechanisms underlying the regulation of ER retrieval in plants remain largely unknown. Plant ERD2-1ike proteins （ERD2s） were recently suggested to function as ER luminal protein receptors that mediate ER retrieval. Here, we demon- strate that heterotrimeric G protein signaling is involved in ERD2-mediated ER retrieval. We show that ERD2s interact with the heterotrimeric G protein Gα and Gγsubunits at the Golgi. Silencing of Gα, Gβ, or Gγ increased the retention of ER luminal proteins. Furthermore, overexpression of Gα, Gβ, or Gγ caused ER luminal proteins to escape from the ER, as did the co-silencing of ERD2a and ERD2b. These results suggest that G proteins interact with ER luminal protein receptors to regulate ER retrieval.

关键词： Chun-Ming Liu institute of Crop Science CAAS institute of botany CAS China

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